Abstract
Stromal membranes enriched in PS I contain a low potential cytochrome with a reduced α-band peak close to 560 nm. The identity of this cytochrome component has been ascribed either to a low potential form of the Photosystem II cytochrome b-559 or to a different cytochrome with a reduced α-band of 560 nm. The half-bandwidth of the 560 nm component in stromal membranes is identical to that of purified cytochrome b-559. Western blots show that the stromal membranes contain an amount of PS II cytochrome b-559 α-subunit that is more than sufficient to account for the cytochrome b-560 detected spectrophotometrically in these membranes. These immunochemical data and the similarity of (i) the spectral peaks, and (ii) the redox properties of low potential PS II cytochrome b-559 and the b-560 component, suggest that the simplest inference is that the cytochrome b-560 protein in stromal membranes is identical to the PS II cytochrome b-559.
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Abbreviations
- A:
-
absorbance
- cyt:
-
cytochrome
- DCBQ:
-
2,5-dichloro-p-benzoquinone
- Emx :
-
midpoint potential at pH x
- hbw:
-
half-bandwidth
- LP:
-
low potential
- MD:
-
menadiol
- MES:
-
2-(N-morpholino)ethanesulfonic acid
- MHQ:
-
methylhydroquinone
- PS I-PS II:
-
photosystems I, II
- SDS-PAGE:
-
sodium dodecylsulfate polyacrylamide gel electrophoresis
References
Anderson JM and Boardman NK (1973) Localization of low potential cytochrome b-559 in Photosystem I. FEBS Lett 32: 157–160
Aro E-M, Hundal T, Carlberg I and Andersson B (1990) In vitro studies on light-induced inhibition of Photosystem II and D1-protein degradation at low temperatures. Biochim Biophys Acta 1019: 269–275
Atteia A, de Vitry C, Pierre Y and Popot J-L (1992) Identification of mitochondrial proteins in membrane preparations from Chlamydomonas reinhardtii. J Biol Chem 267: 226–234
Baroli I, Fernandez-Velasco J and Crofts AR (1991) Spectral and thermodynamic characterization of cytochromes b in stroma vesicles from spinach chloroplasts. Biophys J 59: 145a.
Buser CA, Diner BA and Brudvig GW (1992) Reevaluation of the stoichiometry of cytochrome b-559 in Photosystem II and thylakoid membranes. Biochem 31: 11441–11448
Callahan FE, Wergin WP, Nelson N, Edelman M and Mattoo AK (1989) Distribution of thylakoid proteins between stromal and granal lamellae in Spirodela. Plant Physiol 91: 629–635
Cramer WA and Butler WL (1967) Light-induced absorbance changes of two cytochrome b components in the electron-transport system of spinach chloroplasts. Biochim Biophys Acta 143: 332–339
Cramer WA and Whitmarsh J (1977) Photosynthetic cytochromes. Ann Rev Pl Physiol 28: 133–172
Cramer WA, Theg S and Widger WR (1986) On the structure and function of cytochrome b-559. Photosynth Res 10: 393–403
Deisenhofer J and Michel H (1989) The photosynthetic reaction center from the purple bacterium Rhodopseudomonas viridis. EMBO J 8: 2149–2169
Heber U, Boardman NK and Anderson JM (1976) Cytochrome b-563 redox changes in intact CO2-fixing spinach chloroplasts and in developing pea chloroplasts. Biochim Biophys Acta 423: 275–292
Horton P, Whitmarsh J and Cramer WA (1976) On the specific site of action of 3-(3,4-dichlorophenyl)-1,1-dimethylurea in chloroplasts: Inhibition of a dark acid-induced decrease in midpoint potential. Arch Biochem Biophys 176: 519–524
Hundal T, Virgin I, Styring S and Andersson B (1990) Changes in the organization of Photosystem II following light-induced D1-protein degradation. Biochim Biophys Acta 1017: 235–241
Hurt E and Hauska G (1981) A cytochrome f/b 6 complex of five polypeptides with plastoquinol-plastocyanin-oxidoreductase activity from spinach chloroplasts. Eur J Biochem 117: 591–599
Hurt E and Hauska G (1982) Identification of the polypeptides in the cytochrome b 6 f complex from spinach chloroplasts with redox-center-carrying subunits. J Bioenerg Biomem 14: 405–424
Mattoo AK and Edelman M (1987) Intramembrane translocation and posttranslational palmitoylation of the chloroplast of 32 kDa herbicide-binding protein. Proc Natl Acad Sci USA 84: 1497–1501
Melis A (1991) Dynamics of photosynthetic membrane composition and function. Biochim Biophys Acta 1058: 87–106
Nanba O and Satoh K (1987) Isolation of a Photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559. Proc Natl Acad Sci USA 84: 109–112
Nelson N and Neumann J (1972) Isolation of cytochrome b 6 f particle from chloroplasts. J Biol Chem 247: 1817–1824
Peters FALJ, van Wielink JE, Sang HWWF, de Vries S and Kraayenhof R (1983) Studies on well coupled Photosystem I-enriched subchloroplast vesicles content and redox properties of electron-transfer components. Biochim Biophys Acta 722: 460–470
Porra RJ, Thompson WA and Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with different solvents. Biochim Biophys Acta 975: 384–394
Rich PR and Bendall DS (1980) The redox potentials of the b-type cytochromes of higher plant chloroplasts. Biochim Biophys Acta 591: 153–161
Rich PR, Heathcote P and Moss DA (1987) Kinetic studies of electron transfer in a hybrid system constructed from the cytochrome bf complex and Photosystem I. Biochim Biophys Acta 892: 138–151
Rolfe SA, Sanguansermsri M and Bendall DS (1987) Cytochrome b-560, a new component of thylakoid membranes. Biochim Biophys Acta 894: 434–442
Tae G-S, Black MT, Cramer WA, Vallon O and Bogorad L (1988) Thylakoid membrane protein topography: Transmembrane orientation of the chloroplast cytochrome b-559 psbE gene product. Biochemistry 27: 9075–9080
Vallon O, Hoyer-Hansen G and Simpson DJ (1987) Photosystem II and cytochrome b-559 in the stroma lamellae of barley chloroplasts. Carlsberg Res Comm 52: 405–421
Whitmarsh J (1986) Mobile electron carriers in thylakoids. In: Staehelin LA and Arntzen CJ (eds) Encyclopedia of Plant Physiology, Vol 19, pp 508–527. Springer-Verlag, Berlin
Widger WR, Cramer WA, Hermodson M, Meyer D and Gullifor M (1984) Purification and partial amino acid sequence of the chloroplast cytochrome b-559. J Biol Chem 259: 3870–3876
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Tae, G.S., Everly, R.M., Cramer, W.A. et al. On the question of the identity of cytochrome b-560 in thylakoid stromal membranes. Photosynth Res 36, 141–146 (1993). https://doi.org/10.1007/BF00016278
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DOI: https://doi.org/10.1007/BF00016278