Abstract
Cytochrome b 6 f complexes, prepared from spinach and Chlamydomonas thylakoids, have been examined for their content of low molecular weight subunits. The spinach complex contains two prominent low molecular weight subunits of 3.7 and 4.1 kD while a single prominent component of 4.5 kD was present in the Chlamydomonas complex. An estimation of the relative stoichiometry of these subunits suggests several are present at levels approximating one copy per cytochrome complex. The low molecular weight subunits were purified by reversed phase HPLC and N-terminal sequences obtained. Both the spinach and Chlamydomonas cytochrome complexes contain a subunit that is identified as the previously characterized petG gene product (4.8 kD in spinach and 4.1 kD in Chlamydomonas). A second subunit (3.8 kD in spinach and 3.7 kD in Chlamydomonas) appears to be homologous in the two complexes and is likely to be a nuclear gene product. The possible presence of other low molecular weight subunits in these complexes is also considered.
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References
Anderson JM (1986) Photoregulation of the composition, function, and structure of thylakoid membranes. Ann Rev Plant Physiol 37: 93–136
Chain RK (1985) Involvement of plastoquinone and lipids in electron transport reactions mediated by the cytochrome b 6 f complex isolated from spinach. FEBS Lett 180: 321–325
Fong SE and Surzycki SJ (1992) Organization and structure of plastome psbF, psbL, petG and ORF712 genes in Chlamydomonas reinhardtii. Curr Gen 21: 527–530
Haley J and Bogorad L (1989) A 4-kDa maize chloroplast polypeptide associated with the cytochrome b 6 f complex: Subunit 5, encoded by the chloroplast petE gene. Proc Natl Acad Sci USA 86: 1534–1538
Harris EH (1989) The Chlamydomonas Sourcebook. Academic Press, San Diego
Hauska G (1986) Composition and structure of cytochrome bc and b 6 f complexes. In: Staehelin A and Arntzen CJ (eds) Photosynthesis III. Photosynthetic Membranes and Light Harvesting Systems, pp 496–507. Springer-Verlag, Berlin
Hurt E and Hauska G (1981) A cytochrome f/b 6 complex of five polypeptides with plastoquinol-plastocyanin oxidoreductase activity from spinach chloroplasts. Eur J Biochem 117: 591–599
Hurt E and Hauska G (1982) Identification of the polypeptides in the cytochrome b 6 f complex from spinach chloroplasts with redox-carrying subunits. J Bioenerg Biomembr 14: 119–138
Hurt EC, Gabellini N, Shahak Y, Lockau W and Hauska G (1983) Extra proton translocation and membrane potential generation: Universal properties of cytochrome bc 1/b 6 f complexes reconstituted into liposomes. Arch Biochem Biophys 225: 879–885
Ikeuchi M and Inoue Y (1989) Identification of psbI and psbL in cyanobacterial Photosystem II reaction center preparation. FEBS Lett 251: 155–160
Ikeuchi M, Hirano A, Hiyama T and Inoue Y (1990) Polypeptide composition of higher plant photosystem I complex: Identification of psaI, psaJ, and psaK gene products. FEBS Lett 263: 274–278
Lam E (1986) Nearest-neighbor relationships of the constituent polypeptides in plastoquinol-plastocyanin oxidoreductase. Biochim Biophys Acta 848: 324–332
Li L-B, Zou Y-P, Yu L and Yu C-A (1991) The catalytic role of subunit IV of the cytochrome b 6 f complex from spinach chloroplast. Biochim Biophys Acta 1057: 215–222
Ljungdahl P O, Pennoyer J D, Robertson D E and Trumpower B L (1987) Purification of highly active cytochrome bc 1 complexes from phylogenetically diverse species by a single chromatographic procedure. Biochim Biophys Acta 891: 227–241.
Malkin R. (1988) Structure-function studies of photosynthetic cytochrome bc 1 and b 6 f complexes. In: ISI Atlas of Science, pp 57–64. Institute for Scientific Information. Philadelphia
Rich PR (1986) A perspective on Q-cycles. J Bioenerg Biomembr 18: 145–156
Schägger H and von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368–379
Stirewalt VL and Bryant DA. (1989) Molecular cloning and nucleotide sequence of the petG gene of the cyanelle genome of Cyanophora paradoxa. Nucleic Acids Res 17: 10095–10095
Wakabayashi S, Takeda H. Matsubara H, Kim CH and King TE (1982) Identity of the heme-not-containing protein in bovine heart cytochrome c 1 preparation with the protein mediating c 1-c complex formation — a protein with high glutamic acid content. J Biochem 91: 2077–2085
Widger WR and Cramer WA (1991) The Cytochrome b 6 f Complex: In: Bogorad L and Vasil IK (eds) Cell Culture and Somatic Cell Genetics of Plants, Vol 7B: The Molecular Biology of Plastids, pp 149–176. Academic Press, San Diego
Willms I, Malkin R and Chain RK (1987) Oxidation-reduction reactions of cytochrome b 6 in a liposome-incorporated cytochrome b 6 f complex. Arch Biochem Biophys 258: 248–258
Wynn RM, Bertsch J, Bruce BB, and Malkin R (1988) Green algal cytochrome b 6 f complexes: Isolation and characterization from Dunaliella salina, Chlamydomonas reinhardtii and Scenedesmus obliquus. Biochim Biophys Acta 935: 115–122
Zilber AL and Malkin R (1992) Organization and topology of photosystem I subunits. Plant Physiol 99: 901–911
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Schmidt, C.L., Malkin, R. Low molecular weight subunits associated with the cytochrome b 6 f complexes from spinach and Chlamydomonas reinhardtii . Photosynth Res 38, 73–81 (1993). https://doi.org/10.1007/BF00015063
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DOI: https://doi.org/10.1007/BF00015063