Abstract
North Carolina and Bermuda isolates of the green seaweed Ulvaria oxysperma (Kuetz.) Bliding were grown in cross-gradient light-temperature culture to determine the effects of varied photon fluence rates, temperature and photoperiod on their growth and reproduction. Results indicated significant genetic-based variation between the two populations.
Spheroplast were obtained from the vegetative thallus of the North Carolina isolate following enzymatic digestion with abalone gut extract and maceration. Single cell isolates developed new cell walls in 2–3 days and attached to nylon net substrate. Cultured isolates regenerated into juvenile plants with normal development and morphology.
Results are discussed in terms of the suitability of this economically important species for land-based mariculture.
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References
Adamich, M. & B. Hemmingsen. 1980. Protoplast and spheroplast production. In E. Gant (ed.), Handbook of Phycological Methods: Developmental and Cytological Methods, Cambridge Univ. Press, London: 153–157.
Amsler, C. D. 1985. Field and laboratory studies of Giffordia mitchelliae (Phaeophyceae) in North Carolina. Bot. Mar. 28: 295–301.
Berliner, M. 1981. Protoplast of eukaryotic algae. Int. Rev. Cytol. 73: 1–19.
Bliding, C. 1968. A critical survey of European taxa in Ulvales, II. Ulva, Ulvaria, Monostroma, Kommannia. Bot. Notiser. 121: 535–619.
Chen, L. C. M. 1986. Cell development of Porphyra miniata (Rhodophyceae) under axenic culture. Bot. Mar. 29: 435–439.
Cheney, D. P. 1986. Genetic engineering in seaweds: applications and current status. Nova. Hedwigia. 81: 22–29.
Cheney, D. P., E. Mar, N. Saga & J. van der Meer. 1986. Protoplast isolation and cell division in the agar-producing seaweed Gracilaria (Rhodophyta). J. Phycol. 22: 238–243.
Cocking, E. C. 1972. Plant cell protoplasts, isolation and development. Ann. Rev. Pl. Physiol. 23: 29–50.
Edwards, P. 1969. Field and cultural studies on the seasonal periodicity of growth and reproduction of selected benthic marine algae. Contr. Mar. Sci. 14: 59–144.
Edwards, P. 1970. Field and cultural observations on the growth and reproduction of Polysiphonia denudata from Texas. Br. Phycol. J. 5: 145–153.
Edwards, P. 1971. The effects of light intensity, daylength and temperature on the growth and reproduction of Callithamnion byssoides. In B. C. Parker & R. M. Brown (eds), Contributions in Phycology, Allen Press, Lawrence, Kansas: 163–174.
Freshwater, D. W. & D. F. Kapraun. 1986. Field, culture and cytological studies of Porphyra carolinensis Coll et Cox (Bangiales, Rhodophyta) from North Carolina. Jap. J. Phycol. 43: 251–262.
Fujita, Y. & S. Migita; 1985. Isolation and culture of protoplast from some seaweeds. Bull. Fac. Fisheries, Nagasaki Univ. 57: 39–45.
Hughes, J. & M. E. McCully. 1975. The use of an optical brightner in the study of plant structure. Stain Technol. 50: 319–329.
Kapraun, D. F. 1978a. Field and culture studies on growth and reproduction of Callithamnion byssoides (Rhodophyta, Ceramiales) in North Carolina. J. Phycol. 14: 21–24.
Kapraun, D. F. 1978b. Field and culture studies on selected North Carolina Polysiphonia species. Bot. Mar. 21: 143–153.
Kapraun, D. F. 1979. Comparative studies of Polysiphonia urceolata from three North Atlantic sites. Norw. J. Bot. 26: 269–276.
Kapraun, D. F. & E. H. Flynn. 1973. Culture studies of Enteromorpha linza (L.) J. Ag. and Ulvaria oxysperma (Kuetz.) Bliding (Chlorophyceae, Ulvales) from Central America. Phycologia 12: 145–152.
Kapraun, D. F. & D. W. Freshwater. 1987. Karyological studies of five species in the genus Porphyra (Bangiales, Rhodophyta) from the North Atlantic and Mediterranean. Phycologia 26: 82–87.
Kapraun, D. F. & A. J. Lemus. 1987. Field and culture studies of Porphyra spiralis var. amplifolia Oliveira Filho et Coll (Bangiales, Rhodophyta) from Isla de Margarita, Venezuela. Bot. Mar. 30: 483–490.
Kapraun, D. F. & D. G. Luster. 1980. Field and culture studies of Porphyra rosengurdi Coll et Cox (Rhodophyta, Bangiales) from North Carolina. Bot. Mar. 23: 449–457.
Kanai, R. & G. E. Edwards. 1973. Purification of enzymatically isolated mesophyll protoplast from C3, C4, and crassulacean acid metabolism plants using an aqueous dextran-polyethylene plycol two-phase system. Plant Physiol. 52: 484–490.
Lignell, Å. & M. Pedersén. 1986. Spray cultivation of seaweeds with emphasis on their light requirements. Bot. Mar. 29: 509–516.
Marchant, H. J. & L. C. Fowke. Preparation, culture and regeneration of protoplasts from filamentous green algae. Can. J. Bot. 55: 3080–3086.
Millner, P. A., M. E. Callow & L. V. Evans. 1979. Preparation of protoplast from the green alga Enteromorpha intestinalis (L.) Link. Planta (Berlin) 147: 174–177.
Nisizawa, K., H. Noda, R. Kikuchi & T. Watanabe. 1987. The main seaweed foods in Japan. Hydrobiologia 151–152: 5–29.
Ohno, M. 1972. The periodicity of gamete liberation in Monostroma. Proc. Intl. Seaweed Symp. 7: 405–409.
Polne-Fuller, M. & A. Gibor. 1984. Developmental studies in Porphyra. I. Blade differentiation in Porphyra perforata as expressed by morphology, enzymatic digestion, and protoplast regenaration. J. Phycol. 20: 609–616.
Polne-Fuller, M. & A. Gibor. 1987. Tissue culture of seaweeds. In K. Bird & P. H. Benson (eds), Seaweed Cultivation for Renewable Resources. Gas Research Institute, Chicago, Il. (in press).
Polne-Fuller, M., M. Biniaminov & A. Gibor. 1984. Vegetative propagarion of Porphyra perforata. Hydrobiologia 116/117: 308–313.
Polne-Fuller, M., N. Saga & A. Gibor. 1986. Alga] cell, callus, and tissue cultures and selection of algal strains. In W. R. Barclay & R. M. McIntosh (eds), Algal Biomass Technologies: An Interdisciplinary Perspective. Beih. Nova Hedwigia 83: 22–28.
Rheault, R. B. & J. H. Ryther. 1983. Growth, yield and morphology of Ascophyllum nodosum (Phaeophyta) under continuous and intermittent spray culture regimes. J. Phycol. 19: 252–254.
Saga, N. 1984. Isolation of protoplasts from edible seaweeds. Bot. Mag. Tokyo 97: 423–427.
Saga, N. & Y. Sakai. 1984. Isolation of protoplast from Laminaria and Porphyra. Bull. Jap. Soc. Sci. Fish. 50: 1085.
Saga, N., M. Polne-Fuller & A. Gibor. 1986. Protoplasts from seaweeds: production and fusion. Beih. Nova Hedwigia 83: 37–43.
Staddleman, E. J. & H. Kinzel. 1972. Vital staining of plant cells. In E. M. Prescott (ed.), Methods in Cell Physiology 5, Academic Press, New York: 325–372.
Tang, Y. 1982. Isolation and cultivation of the vegetative cells and protoplast of Porphyra suborbiculata. J. Shandong Coll. of Oceano. 12: 37–50.
Xue-wu, L. & M. E. Gordon. 1987. Tissue and cell culture of New Zealand Pterocladia and Porphyra species. Hydrobiologia 151/152: 147–154.
Zhang, D. 1983. Study on the protoplast preparation, culture and fusion of somatic cells from two species of green algae Ulva linza and Monostroma angicava Kjellm. J. Shandong Coll. of Oceano. 13: 57–65.
Zhao, H. & X. Zhan. 1981. Isolation and cultivation of the vegetative cells of Porphyra yezoensis. J. Shandong Coll. of Oceano. 11: 61–66.
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Kapraun, D.F., Sherman, S.G. Strain selection and cell isolation of Ulvaria oxysperma (Kuetz.) Bliding (Chlorophyta) for net cultivation. Hydrobiologia 179, 53–60 (1989). https://doi.org/10.1007/BF00011929
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DOI: https://doi.org/10.1007/BF00011929