Abstract
The root development of barley seedlings grown for one week in an aerated nutrient solution was studied in the presence of dissolved organic matter from an aqueous chestnut leaf litter extract. In particular, the different effects of low and high molecular weight fractions (small molecules: molecular weight <1000; large molecules: >10,000) of the leaf litter extract were examined. In the presence of large molecules root growth was inhibited, an irregular root tip morphology was observed, and Ca and Mg concentrations in the shoots were lower than in control plants. These phytotoxic effects were not caused by the formation of an impermeable layer of large molecules on the root surfaces that lower accessibility for nutrient cations as inferred from voltammetric experiments. A germination assay using spruce seeds, however, indicated allelochemical effects of large molecules, which exhibit a higher aromaticity than the small molecules as indicated by spectroscopic characterisation. In the growth experiments with small molecules, no influence on the root development of barley was evident, but an increase of Ca and Mg in the shoots was detected. During these growth experiments, a large amount of the small molecules, mainly simple phenols and amino acids, disappeared from the nutrient solution. The loss of small molecules was most likely the effect of mineralisation.
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Abbreviations
- DOC:
-
dissolved organic carbon
- DOM:
-
dissolved organic matter
- LLE:
-
leaf litter extract
- MW:
-
molecular weight
- HMDE:
-
hanging mercury drop electrode
References
Batina N and Cosovic B 1987 Adsorption of a mixture of sodium dodecyl sulphate and dodecyl alcohol on a mercury electrode and its effect on the electrochemical processes of cadmium(II) in sodium chloride solution. J. Electroanal. Chem. 227, 129–146.
Behra R and Gall R 1991 Calcium/calmodulin-dependent phosphorylation and the effect of cadmium in cultured fish cells. Comp. Biochem. Physiol. 100C, 191–195.
Blaser P 1994 The role of natural organic matter in the dynamics of metals in forest soils. In Humic Substances in the Global Environment and Implications on Human Health. Eds. N Senesi and T M Miano. pp 943–960. Elsevier Science BV, Amsterdam, the Netherlands.
Blaser P and Sposito G 1987 Spectrofluorometric investigation of trace metal complexation by an aqueous chestnut leaf litter extract. Soil Sci. Soc. Am. J. 51, 612–619.
Blaser P, Sposito G and Holtzclaw K M 1984 Composition and acidic functional group chemistry of an aqueous chestnut leaf litter extract. Soil Sci. Soc. Am. J. 48, 278–283.
Bloomfield C 1964 Organic matter and soil dynamics. In Experimental Pedology. Eds. E G Hallsworth and D V Crawford. pp 257–266. Butterworths, London, UK.
Bond A M 1980 Modern Polarographic Methods in Analytical Chemistry, Marcel Dekker, New York, USA.
Brezonik P L, Brauner P A and Stumm W 1976 Trace metal analysis by anodic stripping voltammetry: Effect of sorption by natural and model organic compounds. Water Res. 10, 605–612.
Bruckert S 1970 Influence des composés organiques solubles sur la pédogenèse en milieu acide. PhD-thesis, Université de Nancy, France.
Buffle J, Deladoey P and Haerdi W 1978 The use of ultrafiltration for the separation and fractionation of organic ligands in fresh waters. Anal. Chim. Acta. 101, 339–357.
Chen Y and Aviad T 1990 Effects of humic substances on plant growth. In Humic Substances in Soil and Crop Sciences. Eds. P MacCarthy, C E Clapp, R L Malcolm and P R Bloom. pp 161–186. American Society of Agronomy, Madison, WI, USA.
Cosovic B 1985 Aquous surface chemistry: Assessment of adsorption characteristics of organic solutes by electrochemical methods. In Chemical Processes in Lakes. Ed. W Stumm. pp 55–80. Wiley Interscience, New York, USA.
Cosovic B and Vojvodic V 1989 Adsorption behaviour of the hydrophobic fraction of organic matter in natural waters. Mar. Chem. 28, 183–189.
Cox J A, Comte M, Malnoe A, Burger D and Stein E A 1984 Mode of action of the regulatory protein calmodulin. In Calcium and its Role in Biology. Ed. H Siegel. pp 216–275. Marcel Dekker, New York, USA.
DeLong W A and Schnitaer M 1955 Investigation on the mobilization and transport of iron in forested soils. I. The capacities of leaf extracts and leachates to react with iron. Soil Sci. Soc. Am. Proc. 19, 360–363.
Dell'Amico C, Masciandro G, Ganni A, Ceccanti B, Garcia C, Hernandez T and Costa F 1994 Effects of specific humic fractions on plant growth. In Humic Substances in the Global Environment and Implications on Human Health. Eds. N Senesi and T M Miano. pp 563–566. Elsevier Science B V, Amsterdam, the Netherlands.
Einhellig F A 1986 Mechanisms and modes of action of allelochemicals. In The Science of Allelopathy. Eds. A R Putnam and C S Tang. pp 171–188. John Wiley and Sons, New York, USA.
Fisher R F 1987 Allelopathy: a potential cause of forest regeneration failure. In Allelochemicals: Role in Agriculture and Forestry. Ed. G R Waller. ACS Symposium Series 330, 176–184. American Chemical Society, Washington, USA.
Grimvall A, Bengtsson M B, Boren H and Wahlström D 1991 Phytotoxic substances in runoff from forested catchment areas. In Humic Substances in the Aquatic and Terrestrial Environment. Eds. B Allard, H Boren and A Grimvall. pp 397–406. Springer, Berlin, Germany.
Jehring H 1974 Elektrosorptionsanalyse mit der Wechselstrompolarographie. Akademie Verlag, Leipzig, Germany.
Leather G R and Einhellig F A 1986 Bioassays in the study of allelopathy. In The Science of Allelopathy. Eds. A R Putnam and C S Tang. pp 133–145. John Wiley and Sons, New York, USA.
Luster J 1990 Cu(II)-Komplexierung durch gelöstes organisches Material in Wasserextrakten aus Laubstreu sowie durch seine Molekularfiltrations- und Umkehrphasen-Chromatographie-Fraktionen. PhD thesis, Swiss Federal Institute of Technology ETH, Zürich, Switzerland.
Luster J, Blaser P and Magyar B 1994 Equilibrum ion exchange method: methodology at low ionic strength and copper(II) complexation by dissolved organic matter in a leaf litter extract. Talanta 41, 1873–1880.
Lutwick L E and DeLong W A 1954 Leachates from decomposing leaves. II. Interaction with soil-forming materials. Can. J. Agric. Sci. 34, 203–213.
Mallik A U 1992 Possible role of allelopathy in growth inhibition of softwood seedlings in Newfoundland. In Allelopathy: Basic and Applied Aspects. Eds. S J H Rizvi and V Rizvi. pp 321–340. Chapman and Hall, London, UK.
Martin R B 1984 Bioinorganic chemistry of calcium. In Calcium and its Rote in Biology. Ed. H Siegel. Metal ions in Biological Systems 17, 1–50. Marcel Dekker, New York, USA.
Müller T, Guggenheim G, Düggelin M and Scheidegger C 1991 Freeze-fracturing for conventional and field emission lowtemperature scanning electron microscopy: The scanning cryo unit SCU 020. J. Microsc. 161, 73–83.
Nilsson M C, Högberg P, Zackrisson O and Fengyou W 1993 Allelopathic effects by Empetrum hermaphroditum on development and nitrogen uptake by roots and mycorrhizae of Pinus silvestris. Can. J. Bot. 71, 620–628
Ochs M, Brunner I, Stumm W and Cosovic B 1993 Effects of root exudates and humic substances on weathering kinetics. Water Air Soil Pollut. 68, 213–229.
Pellissier F 1990 Allelopathie, mycorhization et les difficultés de la régénération naturelle dans les pessières subalpines. PhD thesis, Université de Savoie, Chambéry, France.
Plavsic M and Cosovic B 1991 Study of cadmium interaction with humic acid at the mercury/water interface. Mar. Chem. 36, 39–49.
Plavsic M, Cosovic B and Miletic S 1991 Comparison of the behaviours of copper, cadmium, and lead in the presence of humic acid in sodium chloride solutions. Anal. Chim. Acta 255, 15–21.
Pretsch E, Clerc T, Seibl J and Simon W 1981 Tabellen zur Strukturaufklärung organischer Verbindungen mit spektroskopischen Methoden. Springer Verlag, Berlin, Germany.
Rice E L 1984 Allelopathy. Academic Press, New York, USA.
Rizvi S J H, Haque H, Singh V K and Rizvi V 1992. A discipline called allelopathy. In Allelopathy: Basic and Applied Aspects. Eds. S J H Rizvi and V Rizvi. pp 1–10. Chapman and Hall, London, UK.
Rougier M 1981 Secretory activity of the root cap. In Plant Carbohydrates II: Extracellular Carbohydrates. Eds. W Tanner and F A Loewus. pp 543–574. Springer Verlag, Berlin, Germany.
Scheidegger C, Günthardt-Goerg M, Matyssek R and Hatvani P 1991 Low-temperature scanning electron microscopy of birch leaves after exposure to ozone. J. Microsc. 161, 85–95.
Senesi N, Miano T M, Provenzano M R and Brunetti G 1991 Characterization, differentiation, and classification of humic substances by fluorescence spectroscopy. Soil Sci. 152, 259–271.
Siqueira J O, Muraleedharan, Nair M G, Hammerschmidt R and Safir G R 1991 Significance of phenolic compounds in plantsoil-microbial systems. Crit. Rev. Plant Sci. 10, 63–121.
Tipping E 1990 Interactions of organic acids with inorganic and organic surfaces. In Organic Acids in Aquatic Ecosystems. Eds. E M Perdue and E T Gjessing. pp 209–221. John Wiley and Sons, Chichester, UK.
Vaughan D, Malcolm R E and Ord B G 1985 Influence of humic substances on biochemical processes in plants. In Soil Organic Matter and Biological Activity. Eds. D Vaughan and R E Malcolm. pp 77–108. Kluwer Academic Publishers, Dordrecht, the Netherlands.
Wolfbeis O S 1985 The fluorescence of organic natural products. In Molecular Luminiscence Spectroscopy. Ed. S G Schulman. pp 167–370. John Wiley and Sons, New York, USA.
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Brunner, I., Luster, J., Ochs, M. et al. Phytotoxic effects of the high molecular weight fraction of an aqueous leaf litter extract on barley root development. Plant Soil 178, 83–93 (1996). https://doi.org/10.1007/BF00011166
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DOI: https://doi.org/10.1007/BF00011166