Abstract
The apparent transfer of N from clover to associated grass was evaluated over a four year period both on the basis of harvested herbage and by taking account of changes in N in stubble and root (to 10 cm depth) in swards with perennial ryegrass and three different white clover cultivars differing in leaf size. The large leaved Aran transferred 15% of its nitrogen while Huia transferred 24% and the small leaved Kent Wild White transferred 34%. When changes in stubble and root N were taken into account the percentage of N transferred was calculated to be 5% less than in harvested herbage only, as the small leaved types had proportionately more N in the roots and stolons, but the large leaved type was probably more competitive towards the grass.
Loss of N from clover roots from July to October was compared to that from grass roots in a grass/white clover sward continuously stocked with steers using a method which incorporated tissue turnover and 15N dilution techniques. Less than 1 mg N m-2 d-1 was lost from the grass roots. In contrast 8 mg m-2 d-1 were estimated to be lost from clover roots while 12 mg N m-2 d-1 were assimilated.
It is concluded that clover cultivar and competitive ability on grass have to be taken into account together with the relationship between N turnover in roots and N available for grass growth when modelling N transfer in grass/clover associations.
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References
Butler G W, Greenwood R M and Soper K 1959 Effects of shading and defoliation on the turnover of root and nodule tissue of plants of Trifolium repens, Trifolium pratense and Lotus uliginosus. N.Z. Agric. Res 2, 415–426.
Camlin M S 1981 competitive effects between ten cultivars of perennial ryegrass and three cultivars of white clover grown in association. Grass Forage Sci. 26, 169–178.
Danso S K A, Labandera C, Pastorini D and Curbelo S 1988 Nitrogen fixation in a two year old white clover fescue pasture: influence of nitrogen fertilization. Soil Biol. Biochem. 20, 261–262.
Dubach M and Russelle M P 1994 Forage legume roots and nodules and their role in nitrogen transfer. Agron J. 86, 259–266.
Gillingham A G, Tilman R W, Gregg P E H and Syers J K 1980 Uptake zones for phosphorus in spring by pasture on different strata within a hill paddock. N.Z.J. Agric. Res. 23, 67–74.
Goodman P J and Collison M 1982 Varietal differences in uptake of 32P labelled phosphate in clover plus grass swards and monocultures. Ann. Appl Biol. 100, 559–565.
Goodman P J and Collison M 1986 Effect of three clover varieties on growth, 15N uptake and fixation by ryegrass/white clover mixtures at three sites in Wales. Grass Forage Sci. 41, 191–198.
Goodman P J 1991 Root turnover and nitrogen cycling in low input grass/clover ecosystems. In Plant Root Growth. An Ecological Perspective. Ed. D Atkinson. pp 267–280. Special publication No 10, British Ecological Society, Blackwell, Oxford, UK.
Haystead A and Lowe A G 1977 Nitrogen fixation by white clover in hill pasture. J. Br. Grassl. Soc. 32, 57–63.
Haystead A and Marriott C A 1979 Transfer of legume nitrogen to associated grass. Soil. Biol. Biochem. 11, 99–104.
Haystead A and Marriott C A 1981 Nitrogen fixation and transfer of fixed nitrogen to associated grass. In Sward Measurements Handbook. Ed. J Hodgson et al. pp 229–242. British Grassland Society, Hurley, UK.
Haystead A, Malajczuk N and Grove T S 1988 Underground transfer of nitrogen between pasture plants infected with vesicular-arbuscular mycorrhizal fungi. New Phytol. 108, 417–423.
Ikram A, Jensen E S and Jakobsen I 1994 No significant transfer of N and P from Pueraria phaseoloides to Hevea brasilienis via hyphal links of arbuscular mycorrhiza. Soil Biol. Biochem. 26, 1541–1547.
Laidlaw A S, Christie P and Withers J A 1990 Comparison between isotope dilution and acetylene reduction methods to estimate N2-fixation rate of white clover in grass/clover swards. Grass Forage Sci. 45, 295–308.
Ledgard S F, Brier G J and Upsdell M P 1990 Effect of clover cultivar on production and nitrogen fixation in clover-ryegrass swards under dairy cow grazing. N.Z.J. Agric. Res. 33, 243–249.
Ledgard S F 1991 Transfer of fixed nitrogen from white clover to associated grasses in swards grazed by dairy cows, estimated using 15N methods. Plant and Soil 131, 215–223.
Leon M, Laine P, Curry A and Boucaud J 1995 Increased uptake of native soil nitrogen by roots of Lolium multiflorium Lam. after nitrogen fertilization is explained by a stimulation of the uptake process itself. Plant and Soil 173, 197–203.
Mackie-Dawson L A and Atkinson D 1991 Methodology for the study of roots in field experiments and the interpretation of results. In Plant Root Growth. An Ecological Perspective. Ed. D Atkinson, pp 25–47 Special publication No 10, British Ecological Society. Blackwell, Oxford, UK.
Marriott C A and Haystead A 1993 Nitrogen fixation and transfer. In Sward Measurement Handbook. Second Edition. Eds. A Davies et al. pp 245–264. British Grassland Society, Reading, UK.
McNeill A M and Wood M 1990 Fixation and transfer of nitrogen by white clover to ryegrass. Soil Use Manage. 6, 84–86.
Millard P. Thomas R J and Buckland S T 1990 Nitrogen supply affects the remobilisation of nitrogen for the regrowth of defoliated Lolium perenne L. J. Exp. Bot. 41, 941–947.
MAFF 1986 The analysis of Agricultural Materials. Reference Book 427, 3rd edn. Ministry of Agriculture, Fisheries and Food. HMSO, London, UK. 248p.
MAFF 1988 Fertiliser Recommendations for Agricultural and Horticultural Crops. Reference Book 209, 5th edn. Ministry of Agriculture, Fisheries and Food, HMSO, London, UK. 185p.
Parker R E 1979 Introductory Statistics for Biology. Second Ed. Edward Arnold, London, UK. 121p.
Preston T and Owens J P 1983 Interfacing an automatic elemental analyser with an isotope ratio mass spectrometer: the potential for fully automated total nitrogen and nitrogen-15 analysis. Analyst 108, 971–977.
Rhodes I 1981 The physiological basis of variation in the yield of grass/clover mixtures. In Plant Physiology and Herbage Growth. Ed. C E Wright. pp 149–161. British Grassland Society, Hurley, UK.
Schøering J K 1991 Ammonia emission from the foliage of growing plants, chapter 12. In Trace Gas Emissions by Plants. Eds. T D Sharkey, E A Holland and H A Mooney. pp 267–292. Academic Press, USA.
Simpson J R 1976 Transfer of nitrogen from three pasture legumes under periodic defoliation in a field environment. Aust. J. Exp. Agric. Anim. Husb. 16, 863–869.
Swift G, Morrison M W, Cleland A T, Smith-Taylor C A B and Dickson J M 1992 Comparison of white clover varieties under cutting and grazing. Grass Forage Sci. 47, 8–13.
Ta T C, MacDowall F D H and Faris M A 1986 Excretion of assimilated N fixed by nodules of alfalfa (Medicago sativa). Can. J. Bot. 64, 2063–2067.
Thomas R J, Lagan K A B, Ironside A D and Bolton G R 1988 Measurement of accumulation of N by grass in a continuously grazed upland pasture. In Field Methods in Terrestrial Ecosystem Nutrient Cycling. Eds. J Harrison et al. pp 428–440. Elsevier, Amsterdam, the Netherlands.
Whitehead D C Lockyer D R 1987 The influence of the concentration of gaseous ammonia on its uptake by the leaves of Italian ryegrass, with and without an adequate supply of nitrogen to the roots. J. Exp. Bot. 38, 818–827.
Wilman D 1970 Direct and indirect contribution of nitrogen by white clover in a grass/clover sward. In White Clover. Ed. J. Lowe. pp 233–237. British Grassland Society, Hurley, UK.
Witty J F 1983 Estimating N2-fixation in the field using 15N-labelled fertiliser: some problems and solutions. Soil Biol. Biochem. 15, 631–639.
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Laidlaw, A.S., Christie, P. & Lee, H.W. Effect of white clover cultivar on apparent transfer of nitrogen from clover to grass and estimation of relative turnover rates of nitrogen in roots. Plant Soil 179, 243–253 (1996). https://doi.org/10.1007/BF00009334
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DOI: https://doi.org/10.1007/BF00009334