Abstract
Liver is the main catabolic tissue for low density lipoprotein in rainbow trout (Gjøen and Berg 1992). We have investigated the interaction of LDL with isolated trout liver cells and liver membranes. 125I-TC labelled trout LDL bound to isolated trout liver cells in a time dependent and saturable manner with an apparant Kd of 20.1 μg/ml, suggesting the existence of a specific binding site on the surface of these cells. The binding was Ca2+ dependent assessed by the 50% reduction obtained by 5 mM EDTA. Saturable binding to isolated trout liver membranes could also be demonstrated, but with lower affinity as compared to intact cells. Degradation of 125I-TC-LDL in hepatocytes was also saturable as degradation could be inhibited about 60% by a 100 fold surplus of unlabelled LDL. The rate of degradation increased with temperature up to 20°C. Both cell association (binding + uptake) and degradation were reduced down to 20% of control in the presence of microtubular and lysosomal inhibitors. Hepatic catabolism of trout LDL therefore seems to depend on receptormediated endocytosis, followed by lysosomal degradation.
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Abbreviations
- TC:
-
tyramine cellobiose
- LDL:
-
low density lipoproteins
- MeLDL:
-
methylated low density lipoproteins
- VLDL:
-
very low density lipoproteins
- HDL:
-
high density lipoproteins
- VTG:
-
vitellogenin
- EDTA:
-
ethylenediamine tetraacetic acid
- PBS:
-
phospate buffered saline
- SDS-PAGE:
-
sodium dodecyl sulphatepolyacrylamide gel electrophoresis
- DMPC:
-
L-α-phosphatidylcholine-dimyristoyl
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Gjøen, T., Berg, T. Interaction of low density lipoproteins with liver cells in rainbow trout. Fish Physiol Biochem 10, 465–473 (1993). https://doi.org/10.1007/BF00004601
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DOI: https://doi.org/10.1007/BF00004601