Abstract
Proteins have been extracted from the edible seaweeds Ulva rigida Agardh and Ulva rotundata Bliding using classical or enzymatic procedures. The protocols using NaOH under reductive conditions or a two-phase system (PEG/K2CO3) produced the best protein yields. The cleavage or the limitation of the linkages between proteins and polysaccharides caused by these experimental conditions probably explains the efficiency of these protocols. In SDS PAGE, the protein fraction obtained after NaOH extraction from U. rotundata is characterised by the presence of three major bands with apparent molecular weights of 45 600, 31 800 and 18 600. The protein fraction from U. rigida presents two specific bands with apparent molecular weights of about 27 000 and 12 000. These fractions are mainly rich in aspartic and glutamic acids, alanine, glycine and contain few hydroxyproline residues (0.91–2.44% total amino acid content). The use of cellulase does not significantly improve the extraction of algal proteins in comparison with the blank procedure (without enzymes). The weak accessibility of the substrates in the intact cell wall could explain these experimental data. The improvement of protein yield after the use of the polysaccharidase mixture (β-glucanase, hemicellulase, cellulase) partially confirms this hypothesis.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bliding C (1968) A critical survey of european taxa in Ulvales, II Ulva, Ulvaria, Monostroma, Kornmannia. Bot. Notiser 121: 535–629.
Cabioc'h J, Floc'h JY, Le Toquin A (1992) Algues vertes. In Cabioc'h J, Floc'h JY, Le Toquin A, Boudouresque CF, Meinez A, Verlaque M (eds), Guide des Algues des Mers d'Europe. Delachaux, Niestlé, Paris: 147–159.
Cachot J, Romna LA, Galgani F (1994) In vivo esterase activity in protoplasts as bioassay of environmental quality. Aquat. Bot. 48: 297–312.
Fleurence J (1991) L'habilitation des Algues en alimentation humaine. Le point sur la réglementation française. Industries agricoles et alimentaires 6: 501–502.
Fujiwara-Arasaki T, Mino N, Kuroda M (1984) The protein value in human nutrition of edible marine algae in Japan. Hydrobiologia 116/117: 513–516.
Gayral P, Cosson J (1986) Ulvales. In Gayral P, Cosson J (eds), Connatre et Reconnatre les Algues Marines. Ouest France Rennes: 69–76.
Hames BD (1981) Introduction to PAGE. In Hames BD, Rickwood D (eds) Gel Electrophoresis of proteins: a Practical Approach. IRL Press Limited, Oxford & Washington DC: 1–91.
Hilditch CM, Jones PB, Balding P, Smith AJ, Rogers LJ (1991) Ribulose Biphosphate carboxylases from macroalgae: proteolysis during extraction and properties of the enzyme from Porphyra umbilicalis. Phytochemistry 3: 745–750.
Indergaard M, Minsaas J (1991) Animal and Human Nutrition. In Guiry G, Blunden G (eds), Seaweed Resources in Europe. Uses and Potential. J. Wiley & Sons, New York: 21–64.
Ito K, Hori K (1989) Seaweed, chemical composition and potential food uses. Food Rev. Int. 5: 101–104.
Jordan P, Vilter H (1991) Extraction of proteins from material rich in anionic mucilages: Partition and fractionation of vanadate-dependent bromoperoxidases from the brown algae Laminaria digitata and L. saccharina in aqueous polymer two-phase systems. Biochem. Biophys. Acta 1073: 98–106.
Kadokami K, Yoshida N, Mizusaki K, Noda K, Makisumi S (1990) Some properties of trypsin-like proteases extracted from the seaweed Codium fragile and their purification. Marine Biology 107: 513–517.
Lamport DTA (1969) Hydroxyproline-o-glycosidic linkage of the plant cell wall glycoprotein extensin. Nature 216: 1322–1324.
Le GAll Y, Braud JP, Kloareg B (1990) Protoplast production in Chondrus crispus gametophytes (Gigartinales, Rhodophyta). Plant Cell Rep. 8: 582–585.
Mabeau S, Fleurence J (1993) Seaweed in food products: biochemical and nutritional aspects. Trends Food Sci. Technol. 4: 103–107.
Ochia Y, Katsuragi T, Hashimoto K (1987) Proteins in three seaweeds, “Aosa” Ulva lactuca, “Arame” Eisenia bicyclis, and “Makusa” Gelidium amansii. Nippon Suisan Gakkaishi 53: 1051–1055.
Potin P, Sanseau A, Le Gall Y, Rochas C, Kloareg B (1991) Purification and characterization of a new κ-carrageenase from a marine Cytophage-like bacterium. Eur. J. Biochem. 201: 241–247.
Serot T, Courcoux T, Guillemineau F (1994) Extraction and partial characterization of protein from the green alga Ulva sp. Sciences des Aliments 14: 301–309.
Siegelman HW Kycia JH (1978) Algal biliproteins. In Hellebus JA, Craigie JS (eds), Handbook of Phycological Methods. Physiological and Biochemical Methods. Cambridge U.P., Cambridge: 71–79.
Shieffield DJ, Harry T, Smith AJ, Rogers LJ (1993) Purification and characterization of the vanadium bromoperoxidase from the macroalga Corallina officinalis. Phytochemistry 32: 21–26.
Thompson EW, Preston RD (1967) Proteins in the cell walls of some green algae. Nature 18: 684–685.
Venkataraman LV, Shivashankar S (1979) Studies on the extractibility of proteins from the alga. Arch. Hydrobiol. 56: 114–126.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fleurence, J., Le Coeur, C., Mabeau, S. et al. Comparison of different extractive procedures for proteins from the edible seaweeds Ulva rigida and Ulva rotundata . J Appl Phycol 7, 577–582 (1995). https://doi.org/10.1007/BF00003945
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00003945