Abstract
The organic phosphate pool of some Camargue sediments (South of France) was studied, after removal of inorganic phosphate, with Ca-NTA/dithionite (Fe bound phosphate) and Na-EDTA (Ca bound phosphate). The organic phosphate was divided into an acid soluble organic phosphate fraction (ASOP) and a residual organic phosphate fraction (ROP). The extraction of organic matter with 2.0 M NaOH (90 °C) from ROP yielded considerable quantities of Org-P. In this extract the presence of phytate (inositol hexa phosphate) could be demonstrated using phytase to hydrolyse the phytate. Phytate was shown to account for a considerable part of organic phosphate in sediments of freshwater marsh sediments as well as in the sediment of the brackish/salt water lake ‘Etang de Vaccares’. In laboratory experiments phytate was found to precipitate with all poly-valent cations tested. Furthermore, phytate was found to be strongly adsorbed onto Fe(OOH), which may explain its accumulation and its stability in sediments.
Considerable quantities of ASOP were found; the chemical stucture of this pool remains unknown.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Abbreviations
- ASOP:
-
acid soluble organic phosphate
- CaCO3 ≈ P:
-
calcium bound phosphate
- Fe(OOH):
-
iron hydroxide
- Fe(OOH) ≈ P:
-
iron bound phosphate
- NaOHextr-P:
-
phosphate extracted with NaOH (90 °C) after ASOP extraction.
- Phyt-P:
-
phytate bound phosphate
- ROP:
-
residual organic phosphate
References
Anderson, G., 1964. Investigations on the analysis of inositol hexaphosphate in soils. Trans. 8th Int. Congr. Soil Sci. 11: 394–402.
Anderson, G., 1970. The isolation of nucleoside diphosphates from alkaline extracts of soil. J. Soil Sci. 21: 96–104.
Anderson, G. & R. E. Malcolm, 1974. The nature of alkalisoluble organic phosphates. J. Soil Sci. 25: 282–297.
Barlett, R. & B. James, 1980. Studying dried, stored soil samples — some pitfalls. Soil Sci. Soc. Am. J. 44: 721–724.
Ballou, C. E., E. Vilkas & E. Lederer, 1963. Structural study on the myo-inositol phospholipids ofMycobacterium tuberculosis (var. bovis, BCG). J. Biol. Chem. 238: 69–76.
Boström, B., M. Jansson & C. Forsberg, 1982. Phosphorus release from sediments. Arch. Hydrobiol. Beih. Ergebn. Limnol. 18: 5–59.
Bostrom, B., I. Ahlgren & R. Bell, 1985. Internal nutrient loading in a eutrophic lake, reflected in seasonal variations of some sediment-parameters. Verh. int. Ver. Limnol. 22: 3335–3339.
Cosgrove, D. J., 1966. Detection of isomers of phytic acid in some Scottish and Californian soils. Soil Sci. 102: 42–43.
Cosgrove, D. J. & M. E. Tate, 1963. Occurrence of neoinositol hexaphosphate in soil. Nature 200: 568–569.
De Groot, C. J., 1990. Some remarks on the presence of organic phosphate in the sediment. In D. J. Bonin & H. L. Golterman (eds), Fluxes Between Trophic Levels and Through the Water-Sediment Interface. Developments in Hydrobiology 62. Kluwer Academic Publishers, Dordrecht: 303–311. Reprinted from Hydrobiologia 207.
De Groot, C. J. & H. L. Golterman, 1990. Sequential fractionation of sediment phosphate. Hydrobiologia 192: 143–149.
De Groot, C. J. & C. Van Wijck, 1993. The impact of desiccation of a freshwater marsh (Garcines Nord, Camargue, France) on sediment-water-vegetation interactions. Part 1: The sediment chemistry. In H. L. Golterman (ed.), Sediment-Water Interaction 4. Hydrobiologia 252: 83–94.
De Groot, C. J. & A. C. Fabre, 1993. The impact of desiccation of a freshwater marsh (Garcines Nord, Camargue, France) on sediment-water-vegetation interactions. Part 3: The fractional composition and the phosphate adsorption characteristics of the sediment. In H. L. Golterman (ed.), Sediment-Water Interaction 4. Hydrobiologia 252: 105–116.
Golterman, H. L., Clymo, R. S. & Ohnstad, M. A. M., 1978. Methods for Physical and Chemical Analysis of Freshwaters, IBP Manual No. 8 (2nd edn.). Blackwell Scientific Publications, Oxford, 213 pp.
Golterman, H. L. & Booman, A., 1988. The sequential extraction of Ca- and Fe-bound phosphates. Verh. int. Ver. Limnol. 23: 904–909.
Golterman, H. L., I. De Graaf Bierbrouwer Wurtz & C. J. De Groot, 1991. Phosphorous compounds in sediments: Inorganic aspects. 3th workshop on phosphorus in sediments, Woudschoten, The Netherlands.
Herbes, S. E., H. E. Allen & K. H. Mancy, 1975. Enzymatic characterization of soluble organic phosphorus in lake water. Science 187: 432–434.
Hess, D., 1975. Plant physiology: 333 pp. Springer Verlag New York, Heidelberg, Berlin.
Hieltjes, A. H. M. & L. Lijklema, 1980. Fractionation of inorganic phosphates in calcareous sediments. J. envir. Qual. 9: 405–407.
Holtan, H., L. Kamp-Nielsen & A. O. Stuanes, 1988. Phosphorus in soil, water and sediment: an overview. In G. Persson & M. Jansson (eds), Phosphorus in Freshwater Ecosystems. Developments in Hydrobiology 48. Kluwer Academic Publishers, Dordrecht: 19–34. Reprinted from Hydrobiologia 170.
Islam, A. & B. Ahmed, 1973. Distribution of inositol phosphates, phospholipids and nucleic acids and mineralization of inositol phosphates in some Bangladesh soils. J. Soil Sci. 24: 193–198.
Martin, J. K. & A. J. Wickert, 1966. Soil organic phosphorus IV. Fractionation of organic phosphorus in alkaline soil extracts and the identification of inositol Phosphates. N.Z.J. agric. Res. 9: 529–539.
Moutin, T., B. Picot, M. C. Ximenes & J. Bontoux, 1993. Seasonal variations of P compounds and their concentrations in two coastal lagoons (Herault, France). In H. L. Golterman (ed.), Sediment-Water Interaction 4. Hydrobiologia 252: 45–59.
Psenner, R., R. Pucsko & M. Sager, 1985. Die Fraktionierung organischer und anorganischer Phosphorverbindungen von Sedimenten. Versuch einer Definition ökologisch wichtiger Fraktionen. Arch Hydrolobiol. Suppl. 70: 111–155.
Psenner, R., B. Boström, M. Dinka, K. Petterson, R. Pucsko & M. Sager, 1988. Fractionation of phosphorus in suspended matter and sediment. Arch. Hydrobiol. Beih. Ergebn. Limnol. 30: 98–103.
Omosoto, T. & A. Wild, 1970. Content of inositol phosphates in some English and Nigerian soils. J. Soil Sci. 21: 216–223.
Stephenson, I. L., 1956. Some observations on the microbial activity in remoisted air-dried soils. Pl. Soil 8: 170–182.
Stevenson, F. J., 1982. Humus chemistry; genesis, composition, reactions. John Wiley & Sons, New York, Chichester, Toronto and Singapore.
Tate, K. R., 1985. Soil phosphorus. In D. Vaugham & R. E. Malcolm (eds), Soil Organic Matter and Biological Activity. Kluwer Academic Publishers, Dordrecht.
Williams, C. H. & G. Anderson, 1968. Inositol phosphates in some Australian soils. Aust. J. Soil Res. 6: 121–130.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
De Groot, C.J., Golterman, H.L. On the presence of organic phosphate in some Camargue sediments: evidence for the importance of phytate. Hydrobiologia 252, 117–126 (1993). https://doi.org/10.1007/BF00000133
Issue Date:
DOI: https://doi.org/10.1007/BF00000133