Summary
The effect of atrial natriuretic factor (ANF; 1, 10, 100, 1000 pmol/l) on insulin release from the isolated perfused rat pancreas was studied. ANF weakly augmented the glucose (10 mmol/l)-stimulated insulin release during the second (controls: 100%; 1 pmol/l: 99%; 10 pmol/l: 149%, P<0.05; 100 pmol/l: 111%; 1000 pmol/l 135%), but not the first phase of the secretory response. In contrast, the first, but not the second phase of arginine (10 mmol/l)-stimulated insulin release was significantly enhanced by ANF (1000 pmol/l; controls: 100%; 1000 pmol/l: 235%, P<0.05). The hormone did not influence basal insulin secretion. Our data indicate an insulinotropic effect of ANF on the rat pancreas, which is dependent on the utilized background secretagogues.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bianchi C, Gutowska J, Thibault G, Garcia R, Genest J, Cantin M (1985) Radioauto-graphic localization of 125-I atrial natriuretic factor (ANF) in rat tissues. Histochemistry 82:441–452
Chabot JG, Morel G, Kopelman H, Belles-Isles M, Heisler S (1987) Atrial natriuretic factor and exocrine pancreas: autoradiographic localization of binding sites and ultrastructural evidence of internalization of endogenous ANF. Pancreas 2:404–413
Chabot JG, Morel G, Belles-Isles M, Jeandel L, Heisler S (1988) ANF and exocrine pancreas: ultrastructural autoradiographic localization in acinar cells. Am J Physiol 254: E301-E309
Fehmann HC, Göke B, Göke R, Trautmann ME, Arnold R (1989) Synergistic stimulatory effect of glucagon-like peptide-1 (7–36) amide and glucose-dependent insulin-releasing polypeptide on the endocrine rat pancreas. FEBS Lett 252:109–112
Flatt P, Bailey CJ (1981) Abnormal plasma glucose and insulin responses in heterozygous (ob/t) mice. Diabetologia 20:573–577
Göke R, Göke B, Noll B, Richter R, Fehmann HC, Arnold R (1989) Receptors for atrial natriuretic peptide on isolated rat adipocytes. Biomed Res 10:463–467
Grodsky GM, Batts AA, Bennett LL, Vcella C, McWilliams ND, Smith DF (1963) Effects of carbohydrates on secretion of insulin from isolated rat pancreas. Am J Physiol 205:638–644
Heisler S, Kopelman H, Chabot JG, Morel G (1987) Atrial natriuretic factor and exocrine pancreas: effects on the secretory process. Pancreas 2:243–251
Hirata Y, Tomita M, Yoshini H, Ikeda M (1984) Specific receptors for atrial natriuretic factor (ANF) in cultured vascular smooth muscle cells of rat aorta. Biochem Biophys Res Commun 125:562–568
Hirata Y, Tomita M, Takata S, Imoue I (1985) Specific binding sites for atrial natriuretic peptide (ANP) in cultured mesenchymal nonmyocardial cells from the heart. Biochem Biophys Res Commun 131:222–229
Kuno T, Andresen JW, Kamisaki Y, Waldman SA, Chang LY, Sahekis S, Leitman DC, Nakane M, Murad F (1988) Co-purification of an atrial natriuretic factor receptor and particulate guanylate cyclase from rat lung. J Biol Chem 261:5817–5823
Lindop GBM, Mallon EA, MacIntyre G (1988) Atrial natriuretic peptide in the heart and the pancreas. Histol Histopathol 1:147–154
Napier MA, Vandlen RL, Albers-Schonberg, G, Nutt RF, Brady S, Lyle T, Winquist R, Faison EP, Heinel LA, Blaine EH (1984) Specific membrane receptors for atrial natriuretic factor in renal and vascular tissue. Proc Natl Acad Sci USA 81:5496–5950
Uehlinger E, Weidmann P, Gnaedinger MP, Hasler L, Bachmann C, Shaw S, Hellmueller B, Lang RE (1986) Increase in circulating insulin induced by atrial natriuretic peptide (ANP) in normal humans. J Cardiovasc Pharmacol 8:1122–1129
Verspohl EJ, Kuehn M, Ammon HPT (1988) RINm5F (rat insulinoma) cells possess receptors for atrial natriuretic peptide (ANF) and a functioning cGMP system. Horm Metab Res 20:770–771
Verspohl EJ, Ammon HPT (1989) Atrial natriuretic peptide (ANP) acts via specific binding sites on cGMP system of rat pancreatic islets without affecting insulin release. Naunyn Schmiedebergs Arch Pharmacol 339:348–353
Vollmar AM, Friedrich A, Sinowatz F, Schulz, R (1988) Presence of atrial natriuretic peptide-like material in guinea pig intestine. Peptides 9:965–971
Vuolteenaho O, Arjamaa O, Vakkuri O, Maksniemi T, Mikkilae L, Kangas J, Puurunen J, Ruskoaho H, Leppaeluoto O (1988) Atrial natriuretic peptide (ANF) in rat gastrointestinal tract. FEBS Lett 233:79–82
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fehmann, H.C., Noll, B., Göke, R. et al. Atrial natriuretic factor has a weak insulinotropic action in the isolated perfused rat pancreas. Res. Exp. Med. 190, 253–258 (1990). https://doi.org/10.1007/BF00000030
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00000030