Abstract
The surface water of the marine environment has traditionally been viewed as a nitrogen (N) limited habitat, and this has guided the development of conceptual biogeochemical models focusing largely on the reservoir of nitrate as the critical source of N to sustain primary productivity. However, selected groups of Bacteria, including cyanobacteria, and Archaea can utilize dinitrogen (N2) as an alternative N source. In the marine environment, these microorganisms can have profound effects on net community production processes and can impact the coupling of C-N-P cycles as well as the net oceanic sequestration of atmospheric carbon dioxide. As one component of an integrated ‘Nitrogen Transport and Transformations’ project, we have begun to re-assess our understanding of (1) the biotic sources and rates of N2 fixation in the world’s oceans, (2) the major controls on rates of oceanic N2 fixation, (3) the significance of this N2 fixation for the global carbon cycle and (4) the role of human activities in the alteration of oceanic N2 fixation. Preliminary results indicate that rates of N2 fixation, especially in subtropical and tropical open ocean habitats, have a major role in the global marine N budget. Iron (Fe) bioavailability appears to be an important control and is, therefore, critical in extrapolation to global rates of N2 fixation. Anthropogenic perturbations may alter N2 fixation in coastal environments through habitat destruction and eutrophication, and open ocean N2 fixation may be enhanced by warming and increased stratification of the upper water column. Global anthropogenic and climatic changes may also affect N2 fixation rates, for example by altering dust inputs (i.e. Fe) or by expansion of subtropical boundaries. Some recent estimates of global ocean N2 fixation are in the range of 100−200 Tg N (1−2 × 1014 g N) yr −1, but have large uncertainties. These estimates are nearly an order of magnitude greater than historical, pre-1980 estimates, but approach modern estimates of oceanic denitrification.
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References
Alldredge AL & Silver MW (1982) Abundance and production rates of floating diatom mats (Rhizosolenia castracanei and R. imbricata var. shrubsolei) in the Eastern Pacific Ocean. Mar. Biol. 66: 83–88
Altabet MA (1988) Variations in nitrogen isotopic composition between sinking and suspended particles: implications for nitrogen cycling and particle transformation in the open ocean. Deep-Sea Res. 35: 535–554
Beget JE (1996) Tephrochronology and paleoclimatology of the last interglacial-glacial cycle recorded in Alaskan loess deposits. Quat. Int. 34–36: 121–126
Benson DR (1985) Consumption of atmospheric nitrogen. In: Leadbetter ER & Poindexter JS (Eds) Bacteria in Nature, Volume 1: Bacterial Activities in Perspective (pp 155–198 ). Plenum Press, New York
Bergman B & Carpenter EJ (1991) Nitrogenase confined to randomly distributed trichomes in the marine cyanobacterium Trichodesmium thiebautii. J. Phycol. 27: 158–165
Bergman B, Siddiqui PJA, Carpenter EJ & Peschek GA (1993) Cytochrome oxidase: subcellular distrivution and relationship to nitrogenase expression in the nonheterocystous cyanobacterium Trichodemium thiebautii. Appl. Environ. Microbiol. 59: 3239–3244
Bergman B, Gallon JR, Rai AN & Stal LJ (1997) N2 fixation by non-heterocystous cyanobacteria. FEMS Microbiol. Rev. 19: 139–185
Bishop PE, Jarlenski DML & Hetherington DR (1980) Evidence for an alternative nitrogen fixation system in Azotobacter vinelandii. Proc. Natl. Acad. Sci. USA 77: 7342–7346
Bishop PE & Premakumar R (1992) Alternative nitrogen fixation systems. In: Stacey G, Burris RH & Evans HJ (Eds) Biological Nitrogen Fixation (pp 736–762 ). Chapman and Hall, New York
Borstad GA, Gower JFR & Carpenter EJ (1992) Development of algorithms for remote sensing of Trichodesmium blooms. In: Carpenter EJ, Capone DG & Rueter JG (Eds) Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs (pp 193–210 ). Kluwer Academic Publishers, The Netherlands
Brandes JA, Devol AH, Yoshinari T, Jayakumar DA & Naqvi SWA (1998) Isotopic composition of nitrate in the central Arabian Sea and eastern tropical North Pacific: A tracer for mixing and nitrogen cycles. Limnol. Oceanogr. 43: 1680–1689
Broughton WJ & Pühler A (Eds) (1986) Nitrogen Fixation, Volume 4: Molecular Biology. Clarendon Press, Oxford
Bryceson I & Fay P (1981) Nitrogen fixation in Oscillatoria (Trichodesmium) erythraea in relation to bundle formation and trichome differentiation. Mar. Biol. 61: 159–166
Burns RC & Hardy RWF (1975) Nitrogen fixation in bacteria and higher plants. Springer-Verlag, New York
Burris RH (1991) Nitrogenases. J. Biol. Chem. 266: 9339–9342
Capone DG (1988) Benthic nitrogen fixation. In: Blackburn H & Sorensen J (Eds) Nitrogen
Cycling in Coastal Marine Environments (pp 85–123). John Wiley, New York
Capone DG & Carpenter EJ (1982) Nitrogen fixation in the marine environment. Science 217: 1140–1142
Capone DG & Carpenter EJ (1999) Nitrogen fixation by marine cyanobacteria: Historical and global perspectives. Bull. Inst. Oceanogr. Monaco 19: 235–256
Capone DG, Ferrier MD & Carpenter EJ (1994) Cycling and release of glutamate and glutamine in colonies of the marine planktonic cyanobacterium, Trichodesmium thiebautii. Appl. Environ. Microbiol. 60: 3989–3995
Capone DG, O’Neil JM, Zehr J & Carpenter EJ (1990) Basis for diel variation in nitrogenase activity in the marine planktonic cyanobacterium Trichodesmium thiebautii. Appl. Environ. Microbiol. 56: 3532–3536
Capone DG, Zehr JP, Paerl HW, Bergman B & Carpenter EJ (1997) Trichodesmium a globally significant marine cyanobacterium. Science 276: 1221–1229
Carpenter EJ (1972) Nitrogen fixation by a blue-green epiphyte on pelagic Sargassum. Science 178: 1207–1209
Carpenter EJ (1983) Nitrogen fixation by marine Oscillatoria (Trichodesmium) in the world’s oceans. In: Carpenter EJ & Capone DG (Eds) Nitrogen in the Marine Environment (pp 65–103 ). Academic Press, New York
Carpenter EJ, Bergman B, Dawson R, Siddiqui PJA, Soderback E & Capone DG (1992) Glutamine synthetase and nitrogen cycling in colonies of the marine diazotrophic cyanobacterium, Trichodesmium spp. Appl. Environ. Microbiol. 58: 3122–3129
Carpenter EJ & Capone DG (1992) Nitrogen fixation in Trichodesmium blooms. In: Carpenter EJ, Capone DG & Rueter J (Eds) Marine Pelagic Cyanobacteria: Trichodesmium and Other Diazotrophs (pp 211–217 ). Kluwer Academic Publishers, The Netherlands
Carpenter EJ, Chang J, Cottrell M, Schubauer J, Paerl HW, Bebout BM & Capone DG (1990) Re-evaluation of nitrogenase oxygen-protective mechanisms in the planktonic marine cyanobacterium Trichodesmium. Mar. Ecol. Prog. Ser. 65: 151–158
Carpenter EJ & Culliney JL (1975) Nitrogen fixation in marine shipworms. Science 187: 551–552
Carpenter EJ, Harvey HR, Fry B & Capone DG (1997) Biogeochemical tracers of the marine cyanobacterium Trichodesmium. Deep-Sea Res. 44: 27–38
Carpenter EJ, O’Neil JM, Dawson R, Capone DG, Siddiqui PJA, Roenneberg T & Bergman B (1993) The tropical diazotrophic phytoplankter Trichodesmium: biological characteristics of two common species. Mar. Ecol. Prog. Ser. 95: 295–304
Carpenter EJ & Price CC, IV (1976) Marine Oscillatoria (Trichodesmium): Explanation for aerobic nitrogen fixation without heterocysts. Science 191: 1278–1280
Carpenter EJ & Roenneberg T (1995) The marine planktonic cyanobacteria Trichodesmium spp.: photosynthetic rate measurements in the SW Atlantic Ocean. Mar. Ecol. Prog. Ser. 118: 267–273
Carpenter EJ & Romans, K (1991) Major role of the cyanobacterium Trichodesmium in nutrient cycling in the North Atlantic Ocean. Science 254: 1356–1358
Checkley DM Jr & Miller CA (1989) Nitrogen isotope fractionation by oceanic zooplankton. Deep-Sea Res. 36: 1449–1456
Codispoti L (1989) Phosphorus versus nitrogen limitation of new and export production. In: Berger WH, Smetacek VS & Wefer G (Eds) Productivity in the Ocean: Present and Past (pp 377–394 ). John Wiley & Sons, New York
Cole JJ, Lane JM, Marino R & Howarth RW (1993) Molybdenum assimilation by cyanobacteria and phytoplankton in freshwater and salt water. Limnol. Oceanogr. 38: 25–35
Cragin JH, Herron MM, Langway Jr. CC & Klouda G (1997) Interhemispheric comparison of changes in the composition of atmospheric precipitation during the late Cenozoic era. In: Dunbar MJ (Ed) Polar Oceans, Proceedings of the Polar Oceans Conference (pp 617–641 ). Arctic Institute of North America, Calgary, Alberta
Cullen JJ, Franks PJS, Karl DM & Longhurst A (2001) Physical influences on marine ecosystem dynamics. In: Robinson AR, McCarthy JJ & Rothschild BJ (Eds) The Sea, vol. 12, in press
Davey A & Marchant HJ (1983) Seasonal variation in nitrogen fixation by Nostoc commune Vaucher at the Vestfold Hills, Antarctica. Phycologia 22: 337–385
Dean DR, Bolin JT & Zheng L (1993) Nitrogenase metalloclusters: Structures, organization, and synthesis. J. Bact. 175: 6737–6744
Delwiche CC (1970) The nitrogen cycle. Sci. Amer. 223: 137–146
Delwiche C & Likens G (1977) Global chemical cycles and their alteration by man. Dahlem Konferenzen, Berlin
Deutsch CA, Gruber NP, Key RM, Sarmiento JL & Ganachaud A (2001) Denitrification and N2 fixation in the Pacific Ocean. Global Biogeochem. Cycles 15: 483–506
Dickey TD (1991) The emergence of concurrent high-resolution physical and bio-optical
measurements in the upper ocean and their applications. Rev. Geophys. 29: 383–413
Dore JE, Popp BN, Karl DM & Sansone FJ (1998) A large source of atmospheric nitrous oxide from subtropical North Pacific surface waters. Nature 396: 63–66
Duarte CM (1992) Nutrient concentration of aquatic plants: Patterns across species. Limnol. Oceanogr. 37: 882–889
Dugdale RC & Goering JJ (1967) Uptake of new and regenerated forms of nitrogen in primary productivity. Limnol. Oceanogr. 12: 196–206
Dugdale RC, Menzel DW & Ryther JH (1961) Nitrogen fixation in the Sargasso Sea. Deep-Sea Res. 7: 298–300
Dupouy C (1992) Discoloured waters in the Melanesian archipelago (New Caledonia and Vanuatu). The value of the NIMBUS-7 Coastal Zone Colour Scanner observations. In: Carpenter EJ, Capone DG & Rueter JG (Eds) Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs (pp 177–191 ) Kluwer Academic Publishers, The Netherlands
Dupouy C, Neveux J, Subramaniam A, Mulholland MR, Montoya JP, Campbell L, Carpenter EJ & Capone DG (2000) Satellite captures Trichodesmium blooms in the southwestern tropical Pacific. Eos 81: 13, 15, 16
Dupouy C, Petit M & Dandonneau Y (1988) Satellite detected cyanobacteria bloom in the southwestern tropical Pacific. Int. J. Remote Sens. 9: 389–396
Falkowski PG (1997) Evolution of the nitrogen cycle and its influence on the biological sequestration of CO2 in the ocean. Nature 387: 272–275
Fallik E, Chan Y-K & Robson RL (1991) Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria. J Bact. 173: 365–371
Fanning KA (1989) Influence of atmospheric pollution on nutrient limitation in the ocean. Nature 339: 460–463
Fanning KA (1992) Nutrient provinces in the sea: Concentration ratios, reaction rate ratios, and ideal covariation. J. Geophys. Res. 97: 5693–5712
Fay P (1992) Oxygen relations of nitrogen fixation in cyanobacteria. Microbiol. Rev. 546: 340–373
Fogg GE (1974) Nitrogen fixation. In: Stewart WDP (Ed) Algal Physiology and Biochemistry (pp 560–582 ). Blackwell, Oxford
Fogg GE (1982) Nitrogen cycling in sea waters. Phil. Trans. R. Soc. London 296: 299–576
Fredriksson C & Bergman B (1997) Ultrastructural characterization of cells specialized for nitrogen fixation in a non-heterocystous cyanobcterium, Trichodesmium. Protoplasma 197: 76–85
Gallon JR (1981) The oxygen sensitivity of nitrogenase: a problem for biochemists and microorganisms. Trends Biochem. Sci. 6: 19–23
Gallon JR (1992) Reconciling the incompatible: N2 fixation and 02. Tansley review No. 144. New Phytol. 122: 571–609
Gallon JR & Stal LJ (1992) N2 fixation in non-heterocystous cyanobacteria: An overview. In: Carpenter EJ, Capone DG & Rueter JG (Eds) Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs (pp 115–139 ). Kluwer Academic Publishers, The Netherlands
Galloway JN, Schlesinger WH, Levy II H, Michaels A & Schnoor JL (1995) Nitrogen fixation: Anthropogenic enhancement-environmental response. Global Biogeochem. Cycles 9: 235–252
Gledhill M & Berg CMGVd (1994) Determination of complexation of iron(III) with natural organic complexing ligands in sewater using cathodic stripping voltammetry. Mar. Chem. 47: 41
Glibert PM & Bronk DA (1994) Release of dissolved organic nitrogen by marine diazotrophic cyanobacteria, Trichodesmium spp. Appl. Environ. Microbiol. 60: 3996–4000
Gordon N, Angel DL, Neori A, Kress N & Kimor B (1994) Heterotrophic dinoflagellates with symbiotic cyanobacteria and nitrogen limitation in the Gulf of Aqaba. Mar. Ecol. Prog. Ser. 107: 83–88
Gruber N & Sarmiento JL (1997) Global patterns of marine nitrogen fixation and denitrification. Global Biogeochem. Cycles 11: 235–266
Guerinot ML & Colwell RR (1985) Enumeration, isolation, and characterization of N2-fixing bacteria from seawater. Appl Environ. Microbiol. 50: 350–355
Guerinot ML & Patriquin DG (1981) The association of N2-fixing bacteria with sea urchins. Mar. Biol. 62: 197–207
Guerinot ML, West PA, Lee JV & Colwell RR (1982) Vibrio diazotrophicus sp. nov., a marine nitrogen-fixing bacterium. Int. J. Syst. Bact. 32: 350–357
Hanson RB (1977) Pelagic Sargassum community metabolism: Carbon and nitrogen. J. Exp. Mar. Biol. Ecol. 29: 107–118
Haxo FT, Lewin RA, Lee KW & Li M-R (1987) Fine structure and pigments of Oscillatoria (Trichodesmium) aff. Thiebautii (Cyanophyta) in culture. Phycologia 26: 443–456
Hecky RE, Campbell P & Hendzel LL (1993) The stoichiometry of carbon, nitrogen, and phosphorus in particulate matter of lakes and oceans. Limnol. Oceanogr. 38: 709–724
Hood RR, Michaels AF & Capone DG (2000) Answers sought to the enigma of marine nitrogen fixation. Eos, Trans. Amer. Geophys. Un. 81: 133, 138, 139
Howard JB & Rees DC (1996) Structural basis of biological nitrogen fixation. Chem. Rev. 96: 2965–2982
Howarth RW, Chan F & Marino R (1999) Do top-down and bottom-up controls interact to exclude nitrogen-fixing cyanobacteria from the plankton of estuaries: explorations with a simulation model. Biogeochem. 46: 203–231
Howarth RW & Cole JJ (1985) Molybdenum availability, nitrogen limitation and phytoplankton growth in natural waters. Science 229: 653–655
Howarth RW, Marino R, Lane J & Cole JJ (1988) Nitrogen fixation in freshwater, estuarine, and marine ecosystems. 2. Biogeochemical controls. Limnol. Oceanogr. 33: 688–701
Husar RB, Prospero JM & Stowe LL (1997) Characterization of tropospheric aerosols over the oceans with the NOAA advanced very high resolution radiometer optical thickness operational product. J. Geophys. Res. 102: 16889–16909
Janson S, Bergman B, Carpenter EJ, Giovannoni SJ & Vergin K (1999a) Genetic analysis of natural populations of the marine diazotrophic cyanobacterium Trichodesmium. FEMS Microbiol. Ecol. 30: 57–65
Janson S, Carpenter EJ & Bergman B (1994) Compartmentalization of nitrogenase in a nonheterocystous cyanobacterium Trichodesmium contortum. FEMS Microbiol. Lett. 118: 914
Janson S, Siddiqui PJA, Walsby AE, Romans K, Carpenter EJ & Bergman B (1995) Cytomorphological characterization of the planktonic diazotrophic cyanobacteria Trichodesmium spp. from the Indian Ocean and Caribbean and Sargasso Seas. J. Phycol. 31: 463–477
Janson S, Wouters J, Bergman B & Carpenter EJ (1999b) Host specificity in the Richeliadiatom symbiosis by hetR gene sequence analysis. Environ. Microbiol. 1: 431–438
Johnson KJ, Gordon RM & Coale KH (1997) What controls dissolved iron concentrations in the world ocean? Mar. Chem. 57: 181
Joussaume S (1993) Paleoclimatic tracers: An investigation using an atmospheric general circulation model under ice age conditions–1. Desert dust. J. Geophys. Res. 98: 2767–2805
Kana TM (1993) Rapid oxygen cycling in Trichodesmium thiebautii. Limnol. Oceanogr. 38: 18–24
Karl DM (1999) A sea of change: Biogeochemical variability in the North Pacific subtropical gyre. Ecosystems 2: 181–214
Karl DM (2000) A new source of `new’ nitrogen in the sea. Trends in Microbiol. 8: 301 (Comment section)
Karl DM, Björkman KM, Dore JE, Fujieki L, Hebel DV, Houlihan T, Letelier RM & Tupas LM (2001) Ecological nitrogen-to-phosphorus stoichiometry at Station ALOHA. Deep-Sea Res. II 48: 1529–1566
Karl DM, Letelier R, Hebel DV, Bird DF & Winn CD (1992) Trichodesmium blooms and new nitrogen in the north Pacific gyre. In: Carpenter EJ, Capone DG & Rueter JG (Eds) Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs (pp 219–237 ). Kluwer Academic Publishers, The Netherlands
Karl D, Letelier R, Tupas L, Dore J, Christian J & Hebel D (1997) The role of nitrogen fixation in biogeochemical cycling in the subtropical North Pacific Ocean. Nature 388: 533–538
Karl DM & Tien G (1997) Temporal variability in dissolved phosphorus concentrations in the subtropical North Pacific Ocean. Mar. Chem. 56: 77–96
Keene WC & Savoie DL (1998) The pH of deliquesced sea-salt aerosol in polluted marine air. Geophys. Res. Lett. 25: 2181–2184
Kim J & Rees DC (1994) Nitrogenase and biological nitrogen fixation. Biochem. 33: 389–397
Kirshtein JD, Zehr JP & Paerl HW (1993) Determination of N2 fixation potential in the marine environment: application of the polymerase chain reaction. Mar. Ecol. Prog. Ser. 95: 305–309
Kuchler DA & Jupp DLB (1988) Shuttle photograph captures massive phytoplankton bloom in the Great Barrier Reef. Int. J. Remote Sensing 9: 1299–1301
Letelier RM & Karl DM (1996) Role of Trichodesmium spp. in the productivity of the subtropical North Pacific Ocean. Mar. Ecol. Prog. Ser. 133: 263–273
Letelier RM & Karl DM (1998) Trichodesmium spp. physiology and nutrient fluxes in the North Pacific subtropical gyre. Aquat. Microb. Ecol. 15: 265–276
LeTraon PY (1990) A method for optimal analysis of fields with spatially variable mean. J. Geophys. Res. 95: 13543–13547
Li WKW, Glover HE & Morris I (1980) Physiology of carbon assimilation by Oscillatoria thiebautii in the Caribbean Sea. Limnol. Oceanogr. 25: 447–456
Lin S, Henze S, Lundgren P, Bergman B & Carpenter EJ (1999) Whole-cell immunolocalization of nitrogenase in marine diazotrophic cyanobacteria, Trichodesmium spp. Appl. Environ. Microbiol. 64: 3052–3064
Lipschultz F & Owens NJ (1996) An assessment of nitrogen fixation as a source of nitrogen to the North Atlantic Ocean. Biogeochem. 35: 261–274
Liu K-K, Su M-J, Hsueh C-R & Gong G-C (1996) The nitrogen isotopic composition of nitrate in the Kuroshio Water northwest of Taiwan: Evidence for nitrogen fixation as a source of isotopically light nitrate. Mar. Chem. 54: 273–292
Liu T, An Z, Yuan B & Han J (1985) The loess-paleosol sequence in China and climatic history. Episodes 8: 21–28
Longhurst A (1998) Ecological Geography of the Sea. Academic Press, San Diego, California
Longhurst AR & Harrison WG (1989) The biological pump: profiles of plankton production and consumption in the upper ocean. Prog. Oceanog. 22: 47–123
Lundgren P, Soederbaeck E, Carpenter EJ & Bergman B (2000) Nitrogen fixation and nitrogenase in Katagnymene spp., a non-heterocystous marine cyanobacterium. Submitted to J. Phycol.
Mague TH, Weare NM & Holm-Hansen 0 (1974) Nitrogen fixation in the North Pacific Ocean. Mar. Biol. 24: 109–119
Mahowald N, Kohfeld KE, Hansson M, Balkanski Y, Harrison SP, Prentice IC, Schulz M & Rodhe H (1999) Dust sources and deposition during the last glacial maximum and current climate: A comparison of model results with palaeodata from ice cores and marine sediments. J. Geophys. Res. in press
Martinez L, Silver MW, King JM & Alldredge AL (1983) Nitrogen fixation by floating diatom mats: A source of new nitrogen to oligotrophic ocean waters. Science 221: 152–154
McElroy MB (1976) Chemical processes in the solar system: a kinetic perspective. In: Herschbach D (Ed) MTP International Review of Science (pp 127–211 ). Buttersworth, London
McElroy MB (1983) Marine biological controls on atmospheric CO2 climate. Nature 302: 328–329
Michaels AF, Bates NR, Buesseler KO, Carlson CA & Knap AH (1994) Carbon-cycle imbalances in the Sargasso Sea. Nature 372: 537–540
Michaels AF, Karl DM & Capone D (2001) Redfield stoichiometry, new production and nitrogen fixation. Oceanography (Special JGOFS edition), in press
Michaels AF, Olson D, Sarmiento JL, Ammerman JW, Fanning K, Jahnke R, Knap AH, Lipschultz F & Prospero JM (1996) Inputs, losses and transformations of nitrogen and phosphorus in the pelagic North Atlantic Ocean. Biogeochem. 35: 181–226
Mitsui A, Kumazawa S, Takahashi A, Ikemoto H, Cao S & Arai T (1986) Strategy by which nitrogen-fixing unicellular cyanobacteria grow photoautotrophically. Nature 323: 720–722
Montoya JP, Voss M, Kaehler P & Capone DG (1996) A simple, high precision tracer assay for dinitrogen fixation. Appl. Environ. Microbiol. 62: 986–993
Moore B, Whitley E & Webster TA (1921) Studies of photo-synthesis in marine algae —1. Fixation of carbon and nitrogen from inorganic sources in sea water. 2. Increase of alkalinity of sea water as a measure of photo-synthesis. Proc. Roy. Soc. Lond. B 92: 51–58
Mulholland MR, Ohki K & Capone DG (1999) Nitrogen utilization and metabolism relative to patterns of N2 fixation in cultures of Trichodesmium NIBB 1067. J. Phycol. 35: 977–988
Niemi A (1979) Blue-green algal blooms and N:P ratios in the Baltic Sea. Acta Bot. Fenn. 110: 57–61
Ohki K & Fujita Y (1982) Laboratory culture of the pelagic blue-green alga Trichodesmium thiebautii: Conditions for unialgal culture. Mar. Ecol. Prog. Ser. 7: 185–190
Ohki K & Fujita Y (1988) Aerobic nitrogenase activity measured as acetylene reduction in the marine non-heterocystous cyanobacterium Trichodesmium spp. grown under artificial conditions. Mar. Biol. 98: 111–114
Ohki K, Rueter JG & Fujita Y (1986) Cultures of the pelagic cyanophytes Trichodesmium erythraeum and T. thiebautii in synthetic medium. Mar. Biol. 91: 9–13
Ohki K, Zehr JP, Falkowski PG & Fujita Y (1991) Regulation of nitrogen fixation by different nitrogen sources in the marine non-heterocystous cyanobacterium Trichodesmium sp. NIBB 1067. Arch. Microbiol. 156: 335–337
Owens NJP (1987) Natural variations in 15N in the marine environment. Adv. Mar. Biol. 24: 389–451
Paerl HW (1994) Spatial segregation of CO2 fixation in Trichodesmium sp.: Linkage to N2 fixation potential. J. Phycol. 30: 790–799
Paerl HW (2000) Physical-chemical constraints on cyanobacterial growth in the oceans. International Symposium on Marine Cyanobacteria and Related Organisms, Institut Oceanographique, Paris
Paerl HW & Bebout BM (1988) Direct measurement of 02-depleted microzones in marine Oscillatoria: relation to N2 fixation. Science 241: 442–445
Paerl HW, Bebout BM & Prufert LE (1989a) Bacterial associations with marine Oscillatoria sp. (Trichodesmium sp.) populations: Ecophysiological implications. J. Phycol. 25: 773–784
Paerl HW & Bland PT (1982) Localized tetrazolium reduction in relation to N2 fixation, CO2 fixation, and H2 uptake in aquatic filamentous cyanobacteria. Appl. Environ. Microbiol. 43: 218–226
Paerl HW, Crocker KM & Prufert LE (1987) Limitation of N2 fixation in coastal marine waters: relative importance of molybdenum, iron, phosphorus and organic matter availability. Limnol Oceanogr. 32: 525–536
Paerl HW & Pinckney JL (1996) A mini-review of microbial consortia: their roles in aquatic production and biogeochemical cycling. Microb. Ecol. 31: 225–247
Paerl HW, Priscu JC & Brawner DL (1989b) Immunochemical localization of nitrogenase in marine Trichodesmium aggregates: Relationship to N2 fixation potential. Appl. Environ. Microbiol. 55: 2965–2975
Paerl HW, Prufert-Bebout L & Guo C (1994) Iron-stimulated N2 fixation and growth in natural and cultured populations of the planktonic marine cyanobacterium Trichodesmium sp. Appl. Environ. Microbiol. 60: 1044–1047
Paerl HW & Zehr JP (2000) Marine nitrogen fixation. In: Kirchman DL (Ed) Paerl HW & Zehr JP (pp 387–426 ). Wiley-Liss
Paul EA (1978) Contribution of nitrogen fixation to ecosystem functioning and nitrogen fluxes on a global basis. Ecol. Bull. 26: 282–293
Paulsen DM, Paerl HW & Bishop PE (1991) Evidence that molybdenum-dependent nitrogen fixation is not limited by high sulfate in marine environments. Limnol. Oceanogr. 36: 1325–1334
Platt T, Harrison WG, Lewis MR, Li WKW, Sathyendranath S, Smith RE & Vezina AF (1989) Biological production of the oceans: the case for a consensus. Mar. Ecol. Prog. Ser. 52: 77–88
Platt T & Sathyendranath S (1999) Spatial structure of pelagic ecosystem processes in the global ocean. Ecosystems 2: 384–394
Postgate JR (1982) The Fundamentals of Nitrogen Fixation. Cambridge University Press, Cambridge
Prentice IC & Webb III T (1998) BIOME 6000: Reconstructing global mid-Holocene
vegetation patterns from paleoecological records. J. Biogeogr. 25: 995–1005
Proctor LM (1997) Nitrogen-fixing, photosynthetic, anaerobic bacteria associated with pelagic copepods. Aquat. Microbiol. Ecol. 12: 105–113
Prospero JM, Barrett K, Church T, Dentener F, Duce RA, Galloway JN, Levy II H, Moody J & Quinn P (1996) Atmospheric deposition of nutrients to the North Atlantic basin. Biogeochemistry 35: 27–73
Prospero JM & Nees RT (1986) Impact of the North African drought and El Nino on mineral dust in the Barbados trace winds. Nature 320: 735–738
Prufert-Bebout L, Paerl HW & Lassen C (1993) Growth, nitrogen fixation, and spectral attenuation in cultivated Trichodesmium species. Appl. Environ. Microbiol. 59: 1367–1375
Rasche ME & Seefeldt LC (1997) Reduction of thiocyanate, cyanate, and carbon disulfide by nitrogenase: Kinetic characterization and EPR spectroscopic analysis. Biochem. 36: 8574–8585
Raven JA (1988) The iron and molybdenum use efficiencies of plant growth with different energy, carbon and nitrogen sources. New Phytol. 109: 279–287
Rea DK (1994) The paleoclimatic record provided by eolian deposition in the deep sea: The geologic history of wind. Rev. Geophys. 32: 159–195
Robson RL & Postgate JR (1980) Oxygen and hydrogen in biological nitrogen fixation. Ann. Rev. Microbiol. 34: 183–207
Rue EL & Bruland KW (1995) Complexation of iron(III) by natural ligands in the central North Pacific as determined by a new competitive ligand equilibrium/absorptive cathodic stripping voltammetry method. Mar. Chem. 50: 117–138
Rueter JG, Hutchins DA, Smith RW & Unsworth NL (1992) Iron nutrition of Trichodesmium. In: Carpenter EJ, Capone DG & Rueter JG (Eds) Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs (pp 289–306 ). Kluwer Academic Publishers, The Netherlands
Saino T & Hattori A (1978) Diel variation in nitrogen fixation by a marine blue-green alga, Trichodesmium thiebautii. Deep-Sea Res. 25: 1259–1263
Saino T & Hattori A (1979) Nitrogen fixation by Trichodesmium and its significance in nitrogen cycling in the Kuroshio area and adjacent waters. Proc. 4th CSK Symp. Tokyo
Saino T & Hattori A (1980) 15N natural abundance in oceanic suspended particulate matter. Nature 283: 752–754
Saino T & Hattori A (1987) Geographical variation of the water column distribution of suspended particulate organic nitrogen and its 15N natural abundance in the Pacific and its marginal seas. Deep-Sea Res. 34: 807–827
Scranton MI (1983) The role of the cyanobacterium Oscillatoria (Trichodesmium) thiebautii in the marine hydrogen cycle. Mar. Ecol. Prog. Ser. 11: 79–87
Seefeldt LC, Rasche ME & Ensign SA (1995) Carbonyl sulfide and carbon dioxide as new substrates, and carbon disulfide as a new inhibitor, of nitrogenase. Biochem. 34: 53825389
Siddiqui PJA, Bergman B & Carpenter EJ (1992) Filamentous cyanobacterial associates of the marine planktonic cyanobacterium Trichodesmium. Phycologia 31: 326–337
Siefert RL, Johansen AM & Hoffman MR (1999) Chemical characterization of ambient aerosol collected during the southwest monsoon and intermonsoon seasons over the Arabian Sea: Labile-Fe(II) and other trace metals. J. Geophys. Res. 104: 3511–3526
Siefert RL, Webb SM & Hoffmann MR (1996) Determination of photochemically available iron in ambient aerosol. J. Geophys. Res. 101: 14441–14449
Siegenthaler U & Sarmiento J (1993) Atmospheric carbon dioxide and the oceans. Nature 365: 119–125
Sigman DM, Altabet MA, McCorkle DC, Francois R & Fischer G (1999) The 8 15 N of nitrate in the Southern Ocean: Nitrate consumption in surface waters. Global Biogeochem. Cycles 13: 1149–1166
Sigman DM, Altabet MA, McCorkle DC, Francois R & Fischer G (2000) The 815N of nitrate in the Southern Ocean: Nitrogen cycling and circulation in the ocean interior. J. Geophys. Res. in press
Sigman DM, Altabet MA, Michener RH, McCorkle DC, Fry B & Holmes RM (1997) Natural abundance-level measurement of the nitrogen isotopic composition of oceanic nitrate: An adaptation of the ammonia diffusion method. Mar. Chem. 57: 227–242
Simpson FB & Burris RH (1984) A nitrogen pressure of 50 atmospheres does not prevent evolution of hydrogen by nitrogenase. Science 224: 1095–1097
Smith BE & Eady RR (1992) Metalloclusters of the nitrogenases. Eur. J. Biochem. 205: 1–15 Soderlund R & Rosswall T (1982) The nitrogen cycles. In: Hutzinger O (Ed) The Natural Environment and the Biogeochemical Cycles (pp 61–81 ).
Springer-Verlag, New York Soderlund R & Svensson BH (1976) The global nitrogen cycle. In: Svensson B & Soderlund R (Eds) Nitrogen, Phosphorus and Sulphur-Global Cycles (pp 23–73). SCOPE Report No. 7, Ecological Bulletin No. 21, NFR., Stockholm
Sprent JI & Sprent P (1990) Nitrogen Fixing Organisms: Pure and Applied Aspects. Chapman and Hall, New York
Stal LJ (1995) Physiological ecology of cyanobacteria in microbial mats and other communities. Tansley Review No. 84. New Phytol. 131: 1–32
Stal LJ & Krumbein WE (1985) Oxygen protection of nitrogenase in the aerobically nitrogen fixing, non-heterocystous cyanobacterium Oscillatoria sp. Arch. Microbiol. 143: 72–76
Stal LJ, Staal M & Villbrandt M (1999) Nutrient control of cyanobacterial blooms in the Baltic Sea. Aquat. Microb. Ecol. 18: 165–173
Stewart WDP, Fitzgerald GP & Burris RH (1967) In situ studies on N2 fixation using the acetylene reduction technique. Proc. Natl. Acad. Aci. USA 58: 2071–2078
Subramaniam A & Carpenter EJ (1994) An empirically derived protocol for the detection of blooms of the marine cyanobacterium Trichodesmium using CZCS imagery. Int. J. Remote Sensing 15: 1559–1569
Subramaniam A, Carpenter EJ & Falkowski PG (1999a) Optical properties of the marine diazotrophic cyanobacteria Trichodesmium spp. II. Reflectance model for remote sensing. Limnol. Oceanogr. 44: 618–627
Subramaniam A, Carpenter EJ, Karentz PG & Falkowski D (1999b) Optical properties of the marine diazotrophic cyanobacteria Trichodesmium spp. I. Absorption and spectral photosynthetic characteristics. Limnol. Oceanogr. 44: 608–617
Takahashi T, Feely RA, Weiss RF, Wanninkhof RH, Chipman DW, Sutherland SC & Takahashi TT (1997) Global air-sea flux of CO2: An estimate based on measurements of sea-air pCO2 difference. Proc. Natl. Acad. Sci. USA 94: 8292–8299
Tans PP, Fung IY & Takahashi T (1990) Observational constraints on the global atmospheric CO2 budget. Science 247: 1431–1438
Tassan S (1995) SeaWiFS potential for remote sensing of marine Trichodesmium at sub-bloom concentration. Int. J. Remote Sensing 16: 3619–3627
Toggweiler JR (1999) An ultimate limiting nutrient. Nature 400: 511–512
Trenberth KE & Hoar Tl (1997) El Nino and climate change. Geophys. Res. Lett. 24: 30573060
Tyrrell T (1999) The relative influences of nitrogen and phosphorus on oceanic primary production. Nature 400: 525–531
Urdaci MC, Stal LJ & Marchand M (1988) Occurrence of nitrogen fixation among Vibrio spp. Arch. Microbiol. 150: 224–229
Venrick EL (1974) The distribution and significance of Richelia intracellularis Schmidt in the North Pacific Central Gyre. Limnol. Oceanogr. 19: 437–445
Villareal TA (1991) Nitrogen-fixation by the cyanobacterial symbiont of the diatom genus Hemiaulus. Mar. Ecol. Prog. Ser. 76: 201–204
Villareal TA, Altabet MA & Culver-Rymsza K (1993) Nitrogen transport by vertically migrating diatom mats in the North Pacific Ocean. Nature 363: 709–712
Villareal TA, Pilskaln C, Brzezinski M, Lipschultz F, Dennett M & Gardner GB (1999) Upward transport of oceanic nitrate by migrating diatom mats. Nature 397: 423–425
Vitousek PM, Cassman K, Cleveland C, Crews T, Field CB, Grimm NB, Howarth RW, Marino R, Martinelli L, Rastetter EB & Sprent JI (2002) Towards an ecological understanding of biological nitrogen fixation. Biogeochemistry 57/58: 1–45
Wada E (1980) Nitrogen isotope fractionation and its significance in biogeochemical processes occurring in marine environments. In: Goldberg ED, Horibe Y & Saruhashi K (Eds) Isotope Marine Chemistry (pp 375–398 ). Uchida-Rokakuho, Tokyo
Wada E & Hattori A (1991) Nitrogen in the Sea: Forms, Abundances, and Rate Processes. CRC Press, Boca Raton, FL.
Waterbury JB, Watson SW & Valois FW (1988) Temporal separation of photosynthesis and dinitrogen fixation in the marine unicellular cyanobacterium: Erythrospira marina. Eos 69: 1089
Wu J & Luther GW (1995) Complexation of Fe(III) by natural organic ligands in the northwest Atlantic Ocean by competitive ligand equilibration method and kinetic approach. Mar. Chem. 50: 159–177
Zehr JP (1995) Nitrogen fixation in the marine environment: Why only Trichodesmium In: Joint IR (Ed) Molecular Ecology of Aquatic Microbes (pp 335–364 ). Springer-Verlag, Berlin
Zehr JP, Braun S, Chen YB & Mellon MT (1996) Nitrogen fixation in the marine environment: Relating genetic potential to nitrogenase activity. J. Exp. Mar. Biol. Ecol. 203: 61–73
Zehr JP & Capone DG (1996) Problems and promise of assaying the genetic potential for nitrogen fixation in the marine environment. Microb. Ecol. 32: 263–281
Zehr JP & McReynolds LA (1989) Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii. Appl. Environ. Microbiol. 55: 2522–2526
Zehr JP, Mellon MT & Zani S (1998) New nitrogen-fixing microorganisms detected in oligotrophic oceans by amplification of nitrogenase (nifH) genes. Appl. Environ. Microbiol. 64: 3444–3450
Zehr JP & Paerl H (1998) Nitrogen fixation in the marine environment: Genetic potential and nitrogenase expression. In: Cooksey KE (Ed) Molecular Approaches to the Study of the Ocean (pp 285–301 ). Chapman and Hall, London
Zehr JP, Carpenter EJ & Villareal TA (2000) New perspectives on nitrogen-fixing microorganisms in tropical and subtropical oceans. Trends in Microbiol. 8: 68–73
Zhu XR, Prospero JM & Millero FJ (1997) Diel variability of soluble Fe(II) and soluble total Fe in North African dust in the trade winds at Barbados. J. Geophys. Res. 102: 2129721305
Zhuang G, Yi Z, Duce RA & Brown PR (1992) Link between iron and sulfur suggested by the detection of Fe(II) in remote marine aerosols. Nature 355: 537–539
Zuckermann H, Staal M, Stal LJ, Reuss J, Hekkert SL, Harren F & Parker D (1997) Online monitoring of nitrogenase activity in cyanobacteria by sensitive laser photoacoustic detection of ethylene. Appl. Environ. Microbiol. 63: 4243–4251
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Karl, D. et al. (2002). Dinitrogen fixation in the world’s oceans. In: Boyer, E.W., Howarth, R.W. (eds) The Nitrogen Cycle at Regional to Global Scales. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3405-9_2
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