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Engineering Trierucin into Oilseed Rape by the Introduction of a 1-Acyl-sn-Glycerol-3-Phosphate Acyltransferase from Limnanthes Douglasii

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Physiology, Biochemistry and Molecular Biology of Plant Lipids

Abstract

Currently available cultivars of high erucic acid rape (HEAR) have a theoretical maximum of 66% erucic acid (22:1, Δ13) in their seed oil due to the specificity of the membrane-bound 1-acyl-sn-glycerol-3-phosphate acyltransferase (LPA-AT) enzyme. In HEAR the LPA-AT does not incorporate erucic acid at the s n-2 position of triacylglycerols (TAG) but preferentially incorporates oleic acid (18:1, Δ9), even if this is only a minor component of the total fatty acid pool (1). However, some plant species, e.g Limnanthes, can utilise erucoyl-CoA as a substrate and effectively incorporate erucic acid at the sn-2 position (2, 3).

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References

  1. Bernerth, R., Frentzen, M. (1990) Utilisation of erucoyl-CoA by acyltransferases from developing seeds of Brassica napus (L.) involved in triacylglycerol biosynthesis. Plant Science 67, 21–28.

    Article  CAS  Google Scholar 

  2. Cao, Y.-Z., Oo, K.-C., Huang, A.H.C. (1990) Lysophosphatidate acyltransferase in microsomes from maturing seeds of meadowfoam (Limnanthes alba). Plant Physiol. 94, 1199–1206.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Löhden, I., Frentzen, M. (1992) Triacylglycerol biosynthesis in developing seeds of Tropaeolum majus L. and Limnanthes douglasii R. Br. Planta 188, 215–224.

    Article  PubMed  Google Scholar 

  4. Coleman J. (1990) Characterization of Escherichia coli cells deficient in 1-acyl-sn-glycerol-3phosphate acyltransferase activity. J. Biol. Chem. 265, 17215–17221.

    Article  CAS  PubMed  Google Scholar 

  5. Brown, A.P., Brough, C.L., Kroon, J.T.M., Slabas, A.R. (1995) Identification of a cDNA that encodes a 1-acyl-sn-glycerol-3-phosphate acyltransferase from Limnanthes douglasii. Plant Mol Biol 29, 267–278.

    Article  CAS  PubMed  Google Scholar 

  6. Hanke, C., Wolter, F.P., Coleman, J. Peterek, G., Frentzen, M. (1995) A plant acyltransferase involved in triacylglycerol biosynthesis complements an Escherichia coli sn-l-acylglycerol-3phosphate acyltransferase mutant. Eur J Biochem 232, 806–810.

    CAS  PubMed  Google Scholar 

  7. Brough, C.L., Coventry, J.M.,Christie, W.W., Kroon, J.T.M., Brown, A.P., Barsby, T.L., Slabas, A.R. (1996) Towards the genetic engineering of triacylglycerols of defined fatty acid composition: major changes in erucic acid content at the so-2 position affected by the introduction of a 1-acyl-snglycerol-3-phosphate acyltransferase from Limnanthes douglasii. Molecular Breeding (in press).

    Google Scholar 

  8. Lassner, M.W., Levering, C.K., Davies, H.M, Knutzon, D.S. (1995) Lysophosphatidic acid acyltransferase from meadowfoam mediates insertion of erucic acid at the sn-2 position of triacylglycerol in transgenic rapeseed oil. Plant Physiol. 109, 1389–1394.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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© 1997 Springer Science+Business Media Dordrecht

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Brough, C.L., Coventry, J., Christie, W., Kroon, J., Barsby, T., Slabas, A. (1997). Engineering Trierucin into Oilseed Rape by the Introduction of a 1-Acyl-sn-Glycerol-3-Phosphate Acyltransferase from Limnanthes Douglasii . In: Williams, J.P., Khan, M.U., Lem, N.W. (eds) Physiology, Biochemistry and Molecular Biology of Plant Lipids. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2662-7_124

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  • DOI: https://doi.org/10.1007/978-94-017-2662-7_124

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4784-7

  • Online ISBN: 978-94-017-2662-7

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