Abstract
In this chapter our recent progress is compiled in the evaluation of a tetrad of stereochemically complementary microbial aldolases as powerful and broadly applicable enzymic catalysts for use in asymmetric synthesis. Made readily available by recombinant DNA methodology and being remarkably stable, these enzymes are capable of promoting efficient, highly enantio- and diastereocontrolled C-C bond formations of a type that proves useful in the de novo syntheses of carbohydrates and related polyhydroxy compounds. Applications range from preparations of rare l-configurated sugars to that of branched-chain, unsaturated, or differently substituted sugar derivatives. Further developments have been made towards the control of the stereochemistry of additional chiral centers, in subsequent enzymatic conversions of aldolase generated products, or in the assembly of aldolase-based complex multi-enzyme cascade reactions that exploit renewable resources as precursors for simple one-pot syntheses.
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© 1992 Springer Science+Business Media Dordrecht
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Fessner, WD. (1992). A Building Block Strategy for Asymmetric Synthesis: The DHAP-Aldolases. In: Servi, S. (eds) Microbial Reagents in Organic Synthesis. NATO ASI Series, vol 381. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2444-7_4
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DOI: https://doi.org/10.1007/978-94-011-2444-7_4
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