Abstract
A large number of alkalophilic bacteria isolated from soil was screened for cyclodextrin glycosyltransferase activity. The strain 1-1 identified as a member of the Bacillus firmus/lentus group was shown to secrete a cyclodextrin glycosyltransferase which forms mainly β-cyclodextrin, small amounts of γ-cyclodextrin and no α-cyclodextrin. The gene for this enzyme was cloned in E. coli and the nucleotide sequence was determined. The nucleotide sequence reveals an open reading frame of 2109 bp, corresponding to a protein of 703 amino acids (aa). A signal peptide precedes the native polypeptide which differs from all known analogous structures by a negative net charge in its N-terminal part.
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References
Horikoshi, K., Akiba, T. (1982) in Alkalophilic Microorganisms, Japan Scientific Societies Press, Tokyo.
Nomoto, M., Chen, C.-C, Sheu, D.-C. (1986) Agrie. Biol. Chem. 50, 2701-2707.
Satomura, S., Omichi, K., Ikenaka, T. (1986) Anal. Biochem. 154, 449-453.
Gryczan, T.J., Contente, S., Dubnau, D. (1978) J. Bacteriol. 134, 318-329.
Maniatis, T., Fritsch, E.F., Sambrook, J. (1982) Molecular cloning, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Maxam, A.M., Gilbert, W. (1980) Methods Enzymol. 65, 499-560.
Gray, C.P., Sommer, R., Polke, C, Beck, E., Schaller, H. (1978) Proc. Natl. Acad. Sci. USA 75, 50-53.
Messing, J., Crea, R., Seeburg, P.H. (1981) Nucleic Acids Res. 9, 309-314.
Chen, E.Y., Seeburg, P.H. (1985) DNA 4, 165-170.
Sneath, P.H.A., Mair, N.S., Sharpe, M.E., Holt, J.G. (1986) in Bergey’s Manual of Systematic Bacteriology 2, Williams & Wilkins, Baltimore.
Duffaud, G.D., Lehnhardt, S.K., March, P.E., Inouye, M. (1985) Current Topics in Membrane and Transport 24, 65-103.
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© 1988 Kluwer Academic Publishers
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Schmid, G., Englbrecht, A., Schmid, D. (1988). Cloning and Nucleotide Sequence of a Cyclodextrin Glycosyltransferase Gene from the Alkalophilic Bacillus 1-1. In: Huber, O., Szejtli, J. (eds) Proceedings of the Fourth International Symposium on Cyclodextrins. Advances in Inclusion Science, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2637-0_11
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DOI: https://doi.org/10.1007/978-94-009-2637-0_11
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7690-6
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