Abstract
Genetic markers are useful for identifying and tracking genes in segregating populations and for comparing genetic diversity among populations. Traditionally, genetic linkage maps were generated by combining data from many populations, because only a limited number of markers segregated in any one population. Restriction fragment length polymorphism (RFLP) analysis employs cloned DNA sequences that hybridize to specific regions of the genome, with genetic polymorphisms defined as differences in the length of DNA fragments observed following digestion with restriction endonucleases and Southern blotting procedures. Since the demonstration of their utility for genetic mapping (Botstein et al., 1980), RFLP markers have been widely used for constructing comprehensive linkage maps for many plant and animal species.
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Severson, D.W. (1997). RFLP analysis of insect genomes . In: Crampton, J.M., Beard, C.B., Louis, C. (eds) The Molecular Biology of Insect Disease Vectors. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1535-0_26
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DOI: https://doi.org/10.1007/978-94-009-1535-0_26
Publisher Name: Springer, Dordrecht
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