Summary
The morphology, incidence and distribution of plaques and diffuse amyloid deposits in the brains of seven old dogs (18.5–26.5 years of age) were examined on tissue sections immunocytochemically stained with two monoclonal antibodies to two distinct epitopes of the beta-protein. Amyloid deposits were found in all seven brains examined. Amyloid occurred in three morphological forms: 1. focal amyloid deposits (plaques), 2. large diffuse amyloid deposits and 3. amyloid angiopathy. The number of these deposits was comparable to the numbers of all three types of amyloid deposits seen in the brains of people with Alzheimer’s disease. The number and type of morphological forms of the amyloid deposits depends on topography and the age of the animals. The number of plaques was highest in the brains of the animals 18.5–21 years of age. The oldest animals (21.5, 24 and 26.5 years of age) had a smaller number of amyloid deposits. With age, the number of plaques decreased in superficial layers of the cerebral cortex (II–III) and increased in the deeper layers (IV–VI). In the oldest animals, diffuse amyloid deposits in the deeper layers of the cortex predominated. Our studies suggest that the frequency and the extent of amyloid deposits in the brains of aged dogs are much wider than so far appreciated. It thus appears that aged dogs may be suitable as an animal model for the study of pathomechanisms involved in beta-protein amyloidogenesis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Ball MJ, MacGregor J, Fyfe IM, Rapoport SI, London ED (1983) Paucity of morphological changes in the brains of ageing beagle dogs: further evidence that Alzheimer lesions are unique for primate central nervous system. Neurobiol Aging 4: 127–131
Bancher C, Grundke-Iqbal I, Iqbal K, Kim KS, Wisniewski HM (1989) Immunoreactivity of neuronal lipofuscin with monoclonal antibodies to the amyloid beta-protein. Neurobiol Aging 10: 125–132
Barcikowska M, Wisniewski HM, Bancher C, Grundke-Igbal I (1989) About the presence of paired helical filaments in dystrophic neurites participating in the plaque formation. Acta Neuropathol 78: 225–231
Binder LI, Frankfurter A, Rebhun LI (1985) The distribution of tau in the mammalian central nervous system. J Cell Biol 101: 1371–1378
Cork LC, Powers RE, Selkoe DJ, Davies P, Geyer JJ, Price DL (1988) Neurofibrillary tangles and senile plaques in aged bears. J Neuropathol Exp Neurol 47: 629–641
Crystal H, Dickson D, Fuld P, Masur D, Scott R, Mehler M, Masdeu J, Kawas C, Aronson M, Wolfson L (1988) Clinico-pathologic studies in dementia: nondemented subjects with pathologically confirmed Alzheimer’s disease. Neurology 38: 1682–1687
Dayan AD (1971) Comparative neuropathology of ageing. Brain 94: 31–42
De Lacoste M-C, Sparkman DR, Pollan KS, Hirstein N, Mihailoff GA, White CL (1989) Laminar and regional distribution of plaques and tangles in the cerebral cortex of Alzheimer patients. J Neuropathol Exp Neurol 48: 351 (Abstr 152)
Dickson DW, Farlo J, Davies P, Crystall H, Fuld P, Yen SH (1988) Alzheimer’s disease. A double-labeling immunohistochemical study of senile plaque. Am J Pathol 132: 86–101
Duyckaerts C, Hauw JJ, Bastenaire F Piette F, Poulain C, Rainsard V, JavoyAgid F, Berthaux P (1986) Laminar distribution of neocortical senile plaques in senile dementia of the Alzheimer type. Acta Neuropathol (Berl) 70: 249–256
Glenner GG, Wong CW (1984) Alzheimer’s disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein. Biochem Biophys Res Commun 120: 885–890
Grundke-Iqbal I, Iqbal K, Tung YC, Quinlan M, Wisniewski HM, Binder LI (1986) Abnormal phosphorylation of the microtubule-associated protein T (tau) in Alzheimer cytoskeletal pathology. Proc Natl Acad Sci USA 83: 4913–4917
Grundke-Iqbal I, Iqbal K, George L, Tung YC, Kim KS, Wisniewski HM (1989) Amyloid protein and neurofibrillary tangles coexist in the same neuron in Alzheimer disease. Proc Natl Acad Sci USA 86: 2853–2857
Katzman R, Terry R, DeTeresa R, Brown T, Davies P, Fuld P, Renbing X, Peck A (1988) Clinical, pathological, and neurochemical changes in dementia: a subgroup with preserved mental status and numerous neocortical plaques. Ann Neurol 23: 138–144
Khachaturian ZS (1985) Diagnosis of Alzheimer’s disease. Arch Neurol 42: 1097–1105
Kim KS, Miller DL, Sapienza V J, Chen CM J, Bai C, Grundke-Iqbal I, Currie JR, Wisniewski HM (1988) Production and characterization of monoclonal antibodies reactive to synthetic cerebrovascular amyloid peptide. Neurosci Res Commun 2: 121–130
Kitamoto T, Ogomori K, Tateishi J, Prusiner SB (1987) Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids. Lab Invest 57: 230–236
Kitt CA, Price DL, Struble RG, Cork LC, Wainer BH, Becher MW, Mobley WC (1984) Evidence for cholinergic neurites in senile plaques. Science 226: 1443–1445
Kitt CA, Struble RG, Cork LC, Mobley WC, Walker LC, Joh TH, Price DL (1985) Catecholaminergic neurites in senile plaques in prefrontal cortex of aged nonhuman primates. Neuroscience 16: 105–115
Kosik KS, Orecchio LD, Binder L, Trojanowski JQ, Lee VMY, Lee G (1988) Epitopes that span the tau molecule are shared with paired helical filaments. Neuron 1: 817–825
Mann DMA, Esiri MM (1989) The pattern of acquisition of plaques and tangles in the brains of patients under 50 years of age with Down’s syndrome. J Neurol Sci 89: 169–179
Masters CL, Simms G, Weinman NA, Multhaup G, McDonald BL, Beyruther K (1985) Amyloid plaque core protein in Alzheimer disease and Down’s syndrome. Proc Natl Acad Sci USA 82: 4245–4249
Osetowska E (1966) Etude anatomopathologique sur le cerveau de chiens senile. In: Luthy F, Bischoff A (eds) Proceedings of the Fifth International Congress of Neuropathology. Excerpta Medica, Amsterdam, pp 497–502
Pearson RCA, Esiri MM, Hiorns RW, Wilcock GK, Powell TPS (1985) Anatomical correlates of the distribution of the pathological changes in the neocortex in Alzheimer disease. Proc Natl Acad Sci USA 82: 4531–4534
Price DL, Cork LC, Struble RG, Kitt CA, Price DL Jr, Lehmann J, Hedreen JC (1985) Neuropathological, neurochemical, and behavioral studies of the aging nonhuman primate. In: Davis RT, Leathers CW (eds) Behavior and pathology of aging in Rhesus monkeys. Monographs in primatology, vol 8. Alan R Liss, New York, pp 113–135
Rafalowska J, Barcikowska M, Wen GY, Wisniewski HM (1988) Laminar distribution of neuritic plaques in normal aging, Alzheimer’s disease and Down’s syndrome. Acta Neuropathol 77: 21–25
Selkoe DJ, Bell DS, Podlisny MB, Price DL, Cork LC (1987) Conservation of brain amyloid proteins in aged mammals and humans with Alzheimer’s disease. Science 235: 873–877
Shin R-W, Ogomori K, Kitamoto T, Tateishi J (1989) Increased tau accumulation in senile plaques as a hallmark in Alzheimer’s disease. Am J Pathol 134: 1365–1371
Struble RG, Price DL Jr, Cork LC, Price DL (1985) Senile plaques in cortex of aged normal monkeys. Brain Res 361: 267–275
Tomlinson BE, Blessed G, Roth M (1970) Observations on the brains of demented old people. J Neurol Sci 11: 205–242
Vaughan DW, Peters A (1981) The structure of neuritic plaques in the cerebral cortex of aged rats. J Neuropathol Exp Neurol 40: 472–487
Von Braunmuhl A (1956) Kongophile Angiopathie und senile Plaques bei greisen Hunden. Arch Psychiat Nervenkr 194: 396–414
Walker LC, Kitt CA, Schwam E, Buckwald B, Garcia F, Sepinwall J, Price DL (1987) Senile plaques in aged squirrel monkeys. Neurobiol Aging 8: 291–296
Walker LC, Kitt CA, Struble RG, Cork LC, Price DL (1986) Heterogeneity of neurites in senile plaques of aged rhesus monkeys (Abstract). 16th Annual meeting, Washington, DC, November 9–14, 1986 (Soc Neurosci Abstr 12: 272)
Wisniewski HM, Bancher C, Barcikowska M, Wen GY, Currie J (1989 b) Spectrum of morphological appearance of amyloid deposits in Alzheimer’s disease. Acta Neuropathol 78: 337–347
Wisniewski HM, Ghetti B, Terry RD (1973) Neuritic (senile) plaques and filamentous changes in aged rhesus monkeys. J Neuropathol Exp Neurol 32: 566–584
Wisniewski HM, Johnson AB, Raine CS, Kay WK, Terry RD (1970) Senile plaques and cerebral amyloidosis in aged dogs. Lab Invest 23: 287–296
Wisniewski HM, Merz GS (1985) Neuropathology of the aging brain and dementia of the Alzheimer type. In: Gaitz CM, Samorajski T (eds) Aging 2000: our health care destiny, vol 1. Biomedical issues. Springer, Berlin Heidelberg New York Tokyo, pp 231–243
Wisniewski HM, Terry RD (1973) Morphology of the aging brain, human and animals. In: Ford DH (ed) Progress in brain research, vol 40. Proceedings of a symposium sponsored by the International Society of Psychoneuroendocrinology, and held at Downstate Medical Center, State University of New York, Brooklyn, NY, USA, June 26–29, 1972. Elsevier, Amsterdam, pp 167–186
Wisniewski HM, Wen GY, Kim KS (1989 a) Comparison of four staining meth- ods on the detection of neuritic plaques. Acta Neuropathol 78: 22–27
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag Wien
About this chapter
Cite this chapter
Wisniewski, H.M., Wegiel, J., Morys, J., Bancher, C., Soltysiak, Z., Kim, K.S. (1990). Aged dogs: an animal model to study beta-protein amyloidogenesis. In: Maurer, K., Riederer, P., Beckmann, H. (eds) Alzheimer’s Disease. Epidemiology, Neuropathology, Neurochemistry, and Clinics. Key Topics in Brain Research. Springer, Vienna. https://doi.org/10.1007/978-3-7091-3396-5_15
Download citation
DOI: https://doi.org/10.1007/978-3-7091-3396-5_15
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-82197-8
Online ISBN: 978-3-7091-3396-5
eBook Packages: Springer Book Archive