Abstract
Acidophilic bacteria capable of attacking metal sulfides are readily isolated from sites of natural mineral oxidation. These bacteria have been divided according to their preferred temperatures for growth into three groups: mesophiles, moderate thermophiles and extreme thermophiles. The mesophiles are those bacteria with optimum temperatures of between 25°–40°C and are incapable of growth above 45°C. Mesophilic iron- or sulfur-oxidizing bacteria can be further subdivided into those which are obligately autotrophic and those which are also capable of growth on organic compounds.1 The moderate and extreme thermophiles are described in chapter 12 and heterotrophic bacteria isolated from iron- and sulfur-rich environments in chapter 13. This chapter deals primarily with the acidophilic, iron- or sulfur-oxidizing obligately autotrophic bacteria. Good reviews on these bacteria have been published2 – 4 and this chapter is intended to update and build on these.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Kelly DP, Harrison AP. Genus Thiobacillus Beijerinck 1904b, 597. In: Staley JT, Bryant MP, Pfenning N et al, eds. Bergey’s Manual of Systematic Bacteriology. Vol 3. Baltimore: Williams and Wilkins, 1989: 1842–1858
Brierley CL. Bacterial leaching. Crit Rev Microbiol 1978; 6: 207–262
Lundgren DG, Silver M. Ore leaching by bacteria. Ann Rev Microbiol 1980; 34: 263–283
Kelly DP, Norris PR, Brierley CL. Microbiolgical methods for the extraction and recovery of metals. In: Bull AT, Ellwood DG, Ratledge C, eds. Microbial Technology: Current State and Future Prospects. Cambridge, UK:Cambridge University Press, 1979:263–308
Norris PR. Acidophilic bacteria and their activity in mineral sulfide oxidation. In: Ehrlich HL, Brierley CL, eds. Microbial Mineral Recovery. New York: McGraw-Hill, 1990: 3–27
Hallberg KB, Lindström EB. Characterization of Thiobacillus caldus sp nov., a moderately thermophilic acidophile. Microbiology 1994; 140: 3451–3456
Goebel BM, Stackebrandt E. Cultural and phylogenetic analysis of mixed microbial populations found in natural and commercial bioleaching environments. Appl Environ Microbiol 1994; 60: 1614–1621
Amaro AM, Hallberg K, Lindström EB et al. An immunological assay for detection and enumeration of thermophilic biomining microorganisms. Appl Environ Microbiol 1994; 60:3470–3473
Hallberg KB. PhD dissertation, 1995 University of Umeå, Sweden
Harrison AP. The acidophilic thiobacilli and other acidophilic bacteria that share their habitat. Ann Rev Microbiol 1984; 38: 265–292
Harrison AP. Genomic and physiological diversity amongst strains of Thiobacillus ferrooxidans and genomic comparison with Thiobacillus thiooxidans. Arch Microbiol 1982; 131: 68–76
Goebel BM and Stackebrandt E. Molecular analysis of the microbial biodiversity in a natural acidic environment. In: Jerez CA, Vargas T, Toledo H, Wiertz JV, eds. Biohydrometallurgical Processing. Vol II. Santiago: University of Chile Press, 1995: 43–51
Harrison AP, Norris PR. Leptospiriilum ferrooxidans and similar bacteria: some characteristics and genomic diversity. FEMS Microbiol Lett 1985; 30: 99–102
Sand W, Rohde K, Sobotke B et al. Evaluation of Leptospirillum ferrooxidans for leaching. Appl Environ Microbiol 1992; 58: 85–92
Norris PR. Iron and mineral oxidation with Leptospirillum-like bacteria. In: Rossi G, Torma AE, eds. Recent Progress in Biohydrometallurgy. Iglesias: Associazione Mineraria Sarda, 1983: 83–96
Rawlings DE. Unpublished observations
Tuovinen OH, Niemelä SI, Gyllenberg HG. Effect of mineral nutrients and organic substances on the development of Thiobacillus ferrooxidans. Biotechnol Bioeng 1971; 13: 517–527
Lane DJ, Stahl DA, Olsen GJ et al. Phylogenetic analysis of the genera Thiobacillus and Thiomicrospira by 5S rRNA sequences. J Bacteriol 1985; 163: 75–81
Lane DJ, Harrison AP, Stahl DA et al. Evolutionary relationships amoung sulfur-and iron-oxidizing eubacteria. J Bacteriol 1992; 174: 269–278
Karlin S, Weinstock GM, Brendel V. Bacterial classifications derived from recA protein sequence comparisions. J Bacteriol 1995; 177: 6881–6893
Karlin S, Brocchieri L. Evolutionary conservation of RecA genes in relation to protein structure and function. J Bacteriol 1996; 178: 1881–1894
Brown LD and Rawlings DE. A comparison of the structure of the H+-translocating ATP synthase from Thiobacullus ferrooxidans with those of other organisms. In: Torma AE, Apel ML, Brierley CL, eds. Biohydrometallurgical Technologies. Vol II. Warrendale, Pennsylvania. TMS Press, 1993: 519–528
Amann RI, Ludwig W, Schleifer K-H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 1995; 59: 143–169
Barros MEC, Rawlings DE, Woods DR. Mixotrophic growth of a Thiobacillus ferrooxidans strain. Appl Environ Microbiol 1984; 593-595
Holuigue L, Herrera L, Phillips OM et al. CO2 fixation by mineral-leaching bacteria: characteristics of the ribulose bisphosphate carboxylase-oxygenase of Thiobacillus ferrooxidans. Biotechnol Appl Biochem 1987; 9: 497–505
Kelly DP, Jones CA. Factors affecting metabolism and ferrous iron oxidation in suspensions and batch cultures of Thiobacillus ferrooxidans: relevance to ferric iron leach solution regeneration. In: Murr LE, Brierley JA, Torma AE, eds. Metallurgical Applications of Bacterial Leaching and Related Microbiological Phenomena. New York: Academic Press. 1978: 19–44
Norris PR. Factors affecting bacterial mineral oxidation: the example of carbon dioxide in the context of bacterial diversity. In: Salley J, McCready RGL, Wichlacz PL, eds. Biohydrometallurgy-1989 Ottawa:CANMET 1989: 1–14
Kusano T, Takeshima T, Inoue, C et al. Evidence for two sets of structural genes coding for ribulose biphosphate carboxylase in Thiobacillus ferrooxidans. J Bacteriol 1991; 173: 7313–7323
Kusano T, Sugawara K. Specific binding of Thiobacillus ferrooxidans RbcR to the intergenic sequence between the rbc operon and the rbcR gene. J Bacteriol 1993; 175: 1019–1025
Mackintosh ME. Nitrogen fixation by Thiobacillus ferrooxidans. J Gen Microbiol 1978; 34: 263–283
Pretorius I-M, Rawlings DE, Woods DR. Indentification and cloning of Thiobacillus ferrooxidans structural nifNDK genes in Escherichia coli. Gene 1985; 45: 59–65
Pretorius I-M, Rawlings DE, O’Neill EG et al. Nucleotide sequence of the gene encoding the nitrogenase protein of Thiobacillus ferrooxidans. J Bacteriol 1997; 169: 367–370
Norris PR, Murrell JC, Hinson D. The potential for diazotrophy in iron-and sulfur-oxidizing acidophilic bacteria. Arch Microbiol 1995; 164: 294–300
Lawson EN. Unpubublished observations
Blake RC, Shute EA, Waskovsky J, Harrison AP. Respiratory components in acidophilic bacteria that respire on iron. Geomicrobiol J 1992; 10: 173–192
Blake RC, Shute EA, Greenwood MM et al. Enzymes of aerobic respiration on iron. FEMS Microbiol Rev 1993; 11: 9–18
Blake RC, McGinness S. Electron-transfer proteins of bacteria that respire on iron. In: Torma AE, Apel ML, Brierley CL, eds. Biohydrometallurgical Technologies. Vol II. Warrendale, Pennsylvania: TMS Press, 1993; 616–628
Yamanaka T, Fukumori Y. Molecular aspects of the electron transfer system which participtes in the oxidayion of ferrous iron by Thiobacillus ferrooxidans. FEMS Microbiol Rev 1995; 17:401–413
Mjoli N, Kulpa CF. The identification of a unique outer membrane protein required for iron oxidation in Thiobacillus ferrooxidans. In: Biohydrometallurgy, Norris PR, Kelly DP, eds. Kew Surrey: Science and Technology Letters, 1988: 89–102
Ronk M, Shively JE, Shute EA et al. Amino acid sequence of the blue copper protein rusticyanin from Thiobacillus ferrooxidans Biochemistry 1931; 30:9435–9442
Nunzi F, Woudstra M, Campèse D et al. Amino acid sequence of rusticycanin from Thiobacillus ferrooxidans and its comparison with other blue copper proteins. Biochim Biophys Acta 1993; 1162:28–34
Kusano T, Takeshima C, Sugawara K et al. Molecular cloning of the gene encoding Thiobacillus ferrooxidans Fe(II) oxidase. J Biol Chem 1992; 267: 11242–11247
Blake RC, Shute EA. Respiratory enzymes of Thiobacillus ferrooxidans Kinetic properties of an acid stable iron:rusticyanin oxidoreductase. Biochemistry 1994; 33:9220–9228
Norris PR, Barr DW, Hinson D. Iron and mineral oxidation by acidophilic bacteria: affinities for iron and attachment to pyrite. In: Biohydrometallurgy, Norris PR, Kelly DP, eds. Kew Surrey: Science and Technology Letters, 1988:43–59
Helle U, Onken U. Continuous bacterial leaching of a pyritic flatation concentrate by mixed cultures. In: Biohydrometallurgy, Norris PR, Kelly DP, eds. Kew Surrey: Science and Technology Letters, 1988: 61–75
Pronk JT, Meulenberg R, Hazeu W et al. Oxidation of reduced sulfur compounds by acidophilic thiobacilli. FEMS Microbiol Rev 1990; 75: 293–306
Kuenen JG, Pronk JT, Hazeu W et al. A review of bioenergetics and enzymology of sulfur compound oxidation by acidophilic thiobacilli. In: Torma AE, Apel ML, Brierley CL, eds. Biohydrometallurgical Technologies. Vol II. Warrendale, Pennsylvania: TMS Press, 1993: 487–505
Sugio T, Domatsu C, Munakata O et al. Role of a ferric ion-reducing system in sulfur oxidation of Thiobacillus ferrooxidans. Appl Environ Microbiol 1985; 49a401–1406
Sugio T, White KJ, Shute E et al. Existence of a hydrogen sulfide:ferric ion oxidoreductase in iron-oxidizing bacteria. Appl Environ Microbiol 1992; 58:431–433
Suzuki H, Tanaka T, Tano T et al. Existence of sulfide binding protein in iron-oxidizing bacteria. In: Torma AE, Apel ML, Brierley CL, eds. Biohydrometallurgical Technologies. Vol II. Warrendale, Pennsylvania: TMS Press, 1993:423–431
Sugio T, Tanaka K, Matsugi S et al. Purification and some properties of NADHdependent sulfite reductase from Thiobacillus ferrooxidans. In: Jerez CA, Vargas T, Toledo H, Wiertz JV, eds. Biohydrometallurgical Processing. Vol II. Santiago: University of Chile Press, 1995: 109–117
Pronk JT, Meijer WM, Hazeu W et al. Growth of Thiobacillus ferrooxidans on formic acid. Appl Environ Microbiol 1991; 57: 2057–2062
Alexander B, Leach S, Ingledew WI. The relationship between chemiosmotic parameters and sensitivity to anions and organic acids in the acidophile Thiobacillus ferrooxidans. J Gen Microbiol 1987; 133x171–1179
Drobner E, Huber H, Stetter KO. Thiobacillus ferrooxidans, a facultative hydrogen oxidizer. Appl Environ Microbiol 1990; 56: 2922–2923
DiSpirito AA, Tuovinen OH. Uranous ion oxidation and carbon dioxide fixation by Thiobacillus ferrooxidans. Arch Microbiol 1982; 133: 28–32
Nielsen AM, Beck JV. Chalcocite oxidation and coupled carbon dioxide fixation by Thiobacillus ferrooxidans. Science 1972; 175: 1124–1126
Sugio T, Tsujita Y, Inagaki K et al. Reduction of cupric ions with elemental sulfur by Thiobacillus ferrooxidans Appl Environ Microbiol 1990; 56: 693–696
Sugio T, Hirayama K, Inagaki K et al. Molybdenum oxidation by Thiobacillus ferrooxidans. Appl Environ Microbiol 1992; 58: 1768–1771
Suzuki I, Takeuchi TL, Yuthasastrakosol TD et al. Ferrous iron and sulfur oxidation and ferric iron reduction activities of Thiobacillus ferrooxidans are affected by grwth on ferrous iron, sulfur or a sulfide ore. Appl Environ Microbiol 1990; 56: 1620–1626
Pronk JT, de Bruyn JC, Bos P et al. Anaerobic growth of Thiobacillus ferrooxidans. Appl Environ Microbiol 1992; 58: 2227–2230
Goodman AE, Babij T, Ritchie AIM. Leaching of a sulfide ore by Thiobacillus ferrooxidans under anaerobic conditions. In: Rossi G, Torma AE, eds. Recent Progress in Biohydromeatallurgy. Iglesias: Associazione Mineraria Sarda, 1983: 361–376
Sugio T, Tsujita Y, Katagaki T et al. Reduction of Mo6+ with elemental sulfur by Thiobacillus ferrooxidans. J Bacteriol 1988; 170:5956–5959
Torma AP. The role of Thiobacillus ferrooxidans in hydrometallurgical processes. Adv Biochem Eng 1977; 6: 1–38
Rawlings DE. Restriction enzyme analysis of 16S rRNA genes for the rapid identification of Thiobacillus ferrooxidans, Thiobacillus thiooxidans and Leptospirillum ferrooxidans strains in leaching environments. In: Jerez CA, Vargas T, Toledo H, Wiertz JV, eds. Biohydrometallurgical Processing. Vol II. Santiago: University of Chile Press, 1995: 9–17
Pizarro J, Jedlicki E, Orellana 0 et al. Bacterial populations in samples of bioleached copper ore as revealed by analysis of DNA obtained before and after cultivation. Appt Environ Microbiol 1996; 62: 1323–1328
Garcia A, Jerez CA. Changes of the solid-adhered populations of Thiobacillus ferrooxidans, Leptospirillum ferrooxidans and Thiobacillus thiooxidans in leaching ores as determined by immunological analysis. In: Jerez CA, Vargas T, Toledo H, Wiertz JV, eds. Biohydrometallurgical Processing. Vol II. Santiago: University of Chile Press, 1995: 19–30
Sand W, Gerke T, Hallmann R et al. Sulfur chemistry, biofilm, and the (in)direct attack mechanism-a critical evaulation of bacterial leaching. Appl Microbiol Biotechnol 1995; 43:961–966
Arredondo R, Garcia A, Jerez CA. Partial removal of lipopolysaccharide from Thiobacillus ferroxidans affects its adhesion to solids. Appl Environ Microbiol 1994; 60: 2846–2851
Gerke T, Hallmann R, Sand W. Importance of exopolymers from Thiobacillus ferrooxidans and Leptospirillum ferrooxidans for bioleaching. In: Jerez CA, Vargas T, Toledo H, Wiertz JV, eds. Biohydrometallurgical Processing. Vol I. Santiago: University of Chile Press, 1995: 1–11
Blake RC, Shute EA, Howard, GT. Solubilization of minerals by bacteria: Electrophoretic mobility of Thiobacillus ferrooxidans in the presence of iron, pyrite, and sulfur. Appl Environ Microbiol 1994; 6o:3349-3357
Vian M, Creo C, Dalmastri C et al. Thiobacillus ferrooxidans selection in continuous culture. In Lawrence RW, Branion RMR, Ebner HG, eds. Fundamental and Applied Biohydrometallurgy, Amsterdam:Elsevier Science Publishing. 1986; 395–406
McCready RG. Progress in the bacterial leaching of metals in Canada. In: Biohydrometallurgy, Norris PR, Kelly DP, eds. Kew Surrey: Science and Technology Letters, 1988: 177–195
Norris PR, Parrot L, Marsh RM. Moderately thermophilic mineral-oxidizing bacteria. Biotech Bioeng Sym 1986; 16: 253–262
Sand W, Gerke T, Hallmann R et al. In situ bioleaching of metal sulfides: The importance of Leptospirillum ferrooxidans. In: Torma AE, Wey JE, Lakshmanan VI, eds. Biohydrometallurgical Technologies. Vol I. Warrendale, Pennsylvania: TMS Press 1993: 15–27
Shiratori T, Inoue C, Sugawara K et al. Cloning and expression of Thiobacillus ferrooxidans mercury ion resistance genes in Escherichia coli. J Bacteriol 1989; 171: 3458–3464
Inoue C, Sugawara K, Shiratori T et al. Nucleo tide sequence of the Thiobacillus ferrooxidans chromosomal gene encoding mercuric resistance. Gene 1989;84:47-54
Inoue C, Sugawara K, Kusano T. The merR regulatory gene in Thiobacillus ferrooxidans is spaced apart from the mer structural genes. Mol Microbiol 1991; 5: 2707–2718
Rawlings DE, Woods DR. Development of improved biomining bacteria. In Gaylarde CG, Videla HA, eds. Bioextraction and biodeterioration of metals. Cambridge: Cambridge University Press 1995; 63–84
Rawlings DE, Silver S. Mining with microbes. Bio/Technology 1995; 13: 773–778
Rawlings DE, Deane SM, Butcher B. Unpublished observations
Leong BJY, Dreisinger DB, Branion R et al. The microbiological leaching of a sulfidic copper ore in a strongly saline medium (I): shakeflask and column studies. In: Torma AE, Wey JE, Lakshmanan VI, eds. Biohydrometallurgical Technologies. Vol I. Warrendale, Pennsylvania: TMS Press, 1993: 117–126
Lawson EN, Nicholas CJ, Pellat H. The toxic effects of chloride ions on Thiobacillus ferrooxidans. In: Jerez CA, Vargas T, Toledo H, Wiertz JV, eds. Biohydrometallurgical Processing. Vol I. Santiago: University of Chile Press, 1995: 165–174
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Rawlings, D.E. (1997). Mesophilic, Autotrophic Bioleaching Bacteria: Description, Physiology and Role. In: Rawlings, D.E. (eds) Biomining. Biotechnology Intelligence Unit. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-06111-4_11
Download citation
DOI: https://doi.org/10.1007/978-3-662-06111-4_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-06113-8
Online ISBN: 978-3-662-06111-4
eBook Packages: Springer Book Archive