Abstract
Bioremediation of polluted soil mostly depends on indigenous micro-organisms. The principle of engineered bioremediation is to stimulate those micro-organisms, which are able to degrade xenobiotic substrates (Aelion et al. 1987). The most often reported genera in degradation of anthropogenic pollutants include Ralstonia,Burkholderia, Comamonas, Arthrobacter, Mycobacterium,Nocardia, fluorescent Pseudomonas,Rhodococcus, and Sphingomonas (Haggblom and Valo 1995; Neilson 1995; Commandeur et al. 1995). In response to the introduction of electron acceptors (like oxygen) or nutrients to soil, specific degrader organisms will multiply. However, in spite of a potentially metabolically active biomass, in-situ bioremediation of soils polluted with hydrophobic organic pollutants (HOC) frequently results in slow pollutant degradation rates, high residual concentrations and, as a consequence, in limited clean-up efficiencies (Zhang et al. 1998; Luthy et al. 1994). The unequal spatial distribution of micro-organisms and pollutants in combination with physically retarded substrate diffusion are nowadays generally accepted as key limiting factors for efficient biodegradation of hydrophobic contaminants in soil.
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References
Aelion CM, Swindoll CM, Pfaender FK (1987) Adaptation to and biodegradation of xenobiotic compounds by microbial communities from a pristine aquifer. Appl Environ Microbiol 53: 2212–2217
Aiba S, Moritz V, Someya J, Haung KL (1969) Cultivation of yeast cells by using n-alkanes as the sole carbon source. I Batch culture. J Ferm Technol 47: 203–210
Bai G, Brusseau ML, Miller RM (1997) Influence of a rhamnolipid biosurfactant on the transport of bacteria through a sandy soil. Appl Environ Microbiol 63: 1866–1873
Balkwil DL, Drake GR, Reeves RH, Frederickson JK, White DC, Ringelberg DB, Chandler DP, Romine MF, Kennedy DW, Spadoni CM (1997) Taxonomic study of aromatic-degrading bacteria from deep-terrestrial-subsurface sediments and description of Sphingomonas aromaticivorans sp nov, Sphingomonas subterranea sp nov, and Sphingomonas stygia sp nov Int J Syst Bacteriol 47: 191–201
Bastiaens L, Springael D, Wattiau P, Harms H, Wachter RD, Verachtert H, Diels L (2000) Isolation of new polycyclic aromatic hydrocarbon ( PAH) degrading bacteria using PAH sorbing carriers. Appl Environ Microbiol 66: 1834–1843
Beurskens JEM, Dekker CGC, Johnkhoff J, Pompstra L (1993) Microbial dechlorination of hexachlorobenzene in a sedimentation area of Rhine river. Biogeochemistry 19: 61–81
Bouchez M, Blanchet D, Vandecasteele JP (1997) An interfacial uptake mechanism for the degradation of pyrene by a Rhodococcus strain. Microbiology 143: 1087–1093
Bosma TNP, Middeldorp PJM, Schraa G, Zehnder AJB (1997) Mass transfer limitation of bio-transformation: Quantifying bioavailability. Environ Sci Technol 31: 248–252
Bouchez M, Blanchet D, Vandecasteele J-P (1995) Substrate availability in phenanthrene bio-degradation: transfer mechanism and influence on metabolism. Appl Microbiol Biotechnol 43: 952–960
Button KD (1985) Kinetics of nutrient-limited transport and microbial growth. Microbiol Rev 49: 270–297.
Calvillo YM, Alexander, M. (1996) Mechanisms of microbial utilization of biphenyl sorbed to polyacrylic beads. Appl. Microbiol. Biotechnol., 45: 383–390
Cameotra SS, Singh HD, Hazarika AK, Baruah JN (1983) Mode of uptake of insoluble solid substrates by microorganisms. II: Uptake of solid n-alkanes by yeast and bacterial species. Biotechnol Bioeng 25: 2945–2956
Crocker FH, Guerin WF, Boyd SA (1995) Bioavailability of naphthalene sorbed to cationic surfactant-modified smectite clay. Environ Sci Technol 29: 2593–2958
Commandeur LCM, Eyseren van HE, Opmeer MR, Govers HAJ, Parsons JR (1995) Biodegradation kinetics of highly chlorinated biphenyls by Alcaligenes sp. JB1 in an aerobic continuous culture system. Environ Sci Technol 29: 3038–3043
Costerton JW, Lappin-Scott HM (1989) Behavior of bacteria in biofilms. ASM news: 650–654 Deware M, Alexander M (1995) Bacterial transport and phenanthrene biodegradation in soil and aquifer sand. Soil Sci Soc Am J 59: 1316–1320
Edmonds RL (1976) Survival of coliform bacteria in sewage sludge applied to a forest clearcut and potential movement into groundwater. Appl Environ Microbiol 32: 537–546
Efroymson RA, Alexander M (1991) Biodegradation by an Arthrobacter species of hydrocarbon partitioned into an organic solvent. Appl Environ Microbiol 57: 1441–1447
Efroymson RA, Alexander M (1994) Role of partitioning in biodegradation of phenanthrene dissolved in nonaqueous-phase liquids. Environ Sci Technol 28: 1172–1179
Flemming HC (1998) Relevance of biofilms for biodeterioration of surfaces of polymeric materials. Polymer Degrad Stabil 59: 309–315
Friedrich M, Grosser RJ, Kern A, Inskeep WP, Ward DM (2000) Effect of model sorptive phases on phenanthrene biodegradation: Molecular analysis of enrichments and isolates suggests selection based on bioavailability. Appl Environ Microbiol 66: 2703–2710
Gosh U, Gillette JS, Luthy R, Zare RN (2000) Microscale location, characterization, and association of polycyclic aromatic hydrocarbons on harbor sediment particles. Environ Sci Technol 34: 1729–1736
Goswami P, Singh HD (1990) Different modes of hydrocarbon uptake by two Pseudomonas species. Biotechnol Bioeng 37: 1–11
Gray MR, Banerjee DK, Fedorak PM, Hashimoto A, Masliyah JH, Pickard MA (1994) Biological remediation of anthracene-contaminated soil in rotating bioreactors. Appl Microbiol Biotechnol 40: 933–940
Gray TRG, Parkinson D (1968) The Ecology of Soil Bacteria. Liverpool University Press, Liverpool, UK
Gross MJ, Logan BE (1995) Influence of different chemical treatments on transport of Alcali-genes paradoxus in porous media. Appl Environ Microbiol 61: 1750–1756
Grosser RJ, Friedrich M, Ward DM, Inskeep WP (2000) Effect of model sorptive phases on phenanthrene biodegradation: different enrichment conditions influence bioavailability and selection of phenanthrene-degrading isolates. Appl Environ Microbiol 66: 2695–2702
Guerin WF, Boyd SA (1992) Differential bioavailability of soil-sorbed naphthalene to two bacterial species. Appl Environ Microbiol 58: 1142–1152
Guerin WF, Boyd SA (1997) Bioavailability of naphthalene associated with natural and synthetic sorbents. Water Res 31: 1504–1512
Häggblom MM, Valo RJ (1995) Bioremediation of chlorophenol wastes. In: Microbial transformation and degradation of toxic organic chemicals (eds Young L, Cerniglia C ), pp 389–434. Wiley-Liss, Inc, New York (NY)
Harms H (1996) Bacterial growth on distant naphthalene diffusing through water, air, water-saturated, and unsaturated porous media. Appl Environ Microbiol 62: 2286–2293
Harms H, Bosma TNP (1997) Mass transfer limitation of microbial growth and pollutant degra-dation. J Ind Microbiol Biotechnol 18: 97–105
Harms H, Zehnder AJB (1994) Influence of substrate diffusion on degradation of dibenzofuran and 3-chlorodibenzofuran by attached and suspended bacteria. Appl Environ Microbiol 60: 2736–2745
Harms H, Zehnder MB (1995) Bioavailability of sorbed 3-chlorodibenzofuran. Appl Environ Microbiol 61: 27–33
Hinchee RE, Fredrickson J, Alleman BC (1995) Bioaugmentation for Site Remediation. Battelle Press, Columbus, Ohio
Kanaly RA, Harayama S (2000) Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons. J Bacteriol 182: 2059–2067
Kilbertus G (1980) Etude des microhabitats contenus dans les aggrégats du soil. Leur relation avec la biomass bactérienne et la taille des procaryotes présents. Rev Ecol Biol Soil 17: 543557
Kirschner-Zilber I, Rosenberg E, Gutnick G (1980) Incorporation of 32P and growth of Pseudomonas UP-2 on n-teracosane. Appl Environ Microbiol 40: 1086–1093
Kleespies M, Kroppenstedt RM, Rainey FA, Webb LE, Stackebrandt E (1996) Mycobacterium hodleri sp. nov., a new member of the fast growing mycobacteria capable of degrading poly-cyclic aromatic hydrocarbons. Int J Syst Bacteriol 46: 683–687
Koch AK, Käppeli 0, Fiechter A, Reiser J (1991) Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants. J Bacteriol 173: 4212–4219
KovTrrovâ-Kovar K, and Egli T (1968) Growth kinetics of suspended microbial cells: from single-substrate-controlled growth to mixed-substrate kinetics. Microbiol Mol Biol Rev 62: 646–666
Laha S, Luthy RG (1991) Inhibition of phenanthrene mineralization by nonionic surfactants in soil-water systems. Environ Sci Technol 25: 1921–1930
Lahlou M, Harms H, Ortega J-J (2000) Influence of individual soil components on the transport of polycyclic aromatic hydrocarbon-degrading bacteria. Environ Sci Technol: in press
Lahlou M, Ortega-Calvo JJ (1999) Bioavailability of labile and desorption-resistant phenanthrene sorbed to montmorillonite clay containing humic acids. Environ Toxicol Chem 18: 2729–2735
Li Q, Logan BE (1999) Enhancing bacterial transport for bioaugmentation of aquifers using low ionic strength solutions and surfactants. Water Research 33: 1090–1100
Linos A, Berekaa MM, Reichelt R, Keller U, Schmitt J, Flemming HC, Kroppenstedt RM, Steinbuchel A (2000) Biodegradation of cis-l,4-polyisoprene rubbers by distinct Actinomycetes: Microbial strategies and detailled surface analysis. Environ Appl Microbiol 66: 16391645
Liu Z, Jacobson AM, Luthy RG (1995) Biodegradation of naphthalene in nonionic surfactant systems. Appl Environ Microbiol 51: 145–151
Luthy RG, Aiken GR, Brusseau ML, Cunningham SD, Gschwend PM, Pignatello JJ, Reinhard M, Traina SJ, Weber WJ, Westall JC (1997) Sequestration of hydrophobic organic contaminants by geosorbents. Environ Sci Technol 31: 3341–3347
Luthy RG, Dzombak DA, Peters CA, Roy SB, Ramaswami A, Nakles DV, Nott BR (1994) Remediating tar-contaminated soils at manufactured gas plant sites. Environ Sci Technol 28: 266A - 277A
Madsen EL, Alexander M (1982) Transport of Rhizobium and Pseudomonas through soil. Soil Sci Am J 46: 557–560
McLee AG, Davies SL (1972) Linear growth of a Torulopsis sp. on n-alkanes. Canad J Microbiol 18: 315–319
van der Mei HC, Rosenberg M, Busscher HJ (1991) Assessment of microbial cell surface hydrophobicity. In: Microbial Cell Surface Analysis (eds N Mozes, PS Handley, HT Busscher, PG Rouxhet ), pp 263–287. VCH Publishers Inc, New York, Weinheim, Cambridge
Mihelcic JR, Luthy RG (1991) Sorption and microbial degradation of naphthalene in soil water suspensions under denitrification conditions. Environ Sci Technol 25: 169–177
Mulder H, Breure AM, Andel JGV, Grotenhuis JT, Rulkens WH (1998) Influence of hydrodynamic conditions on naphthalene dissolution and subsequent biodegradation. Biotechnol Bioeng 57: 145–154
Mulder H, Breure AM, Honschooten Dv, Grotenhuis JT, Andel JGV, Rulkens WH (1998) Effect of biofilm formation by Pseudomonas 8909N on the bioavailability of solid naphthalene. Appl Microbiol Biotechnol 50: 277–283
Neilson AH (1995) An environmental perspective on the biodegradation of organochlorine xenobiotics. Int Biodeteriorat Biodegrad 37: 3–21
Neu TR (1996) Significance of bacterial surface-active compounds in interaction of bacteria with interfaces. Microbiol Reviews 60: 151–166
Noordman WH, Ji W, Brusseau ML, Janssen DB (1998) Effects of rhamnolipid biosurfactants on removal of phenanthrene from soil. Environ Sci Technol 32: 1806–1812
Ogram AV, Jessup RE, Ou LT, Rao PS (1985) Effects of sorption on biological degradation rates of (2,4-dichlorophenoxy)acetic acid in soils. Appl Environ Microbiol 49: 582–587
Ortega-Calvo JJ, Fesch C, Harms H (1999) Biodegradation of sorbed 2,4-dinitrotoluene in a clay-rich, aggregated porous medium. Environ Sci Technol 33: 3737–3742
Ortega-Calvo JJ, Alexander M (1994) Roles of bacterial attachment and spontaneous partitioniing in the biodegradation of naphthalene initially present in nonaqueous-phase liquids. Appl Environ Microbiol 60: 2643–2646
Pignatello JJ, Xing B (1996) Mechanism of slow sorption of organic chemicals to natural particles. Environ Sci Technol 30: 1–11
Pirt SJ (1965) The maintenance energy of bacteria in growing cultures. Proc R Soc B 163: 224231
Postma J, Veen van JA (1990) Habitable pore space and population dynamics of Rhizobium leguminosarium biovar trifolii introduced into soil. Microb Ecol 19: 149–161
Ramaswami A, Ghoshal S, Luthy RG (1997) Mass transfer and bioavailability of PAH com-pounds in coal tar systems. 2. Experimental Evaluations. Environ Sci Technol 31: 2268–2275
Ramaswami A, Luthy RG (1997) Mass transfer and bioavailability of PAH compounds in coal tar systems. 1. Model development. Environ Sci Technol 31: 2260–2267
Rijnaarts HHM, Bachmann A, Jumelet JC, Zehnder AJB (1990) Effect of desorption and intraparticle mass transfer on the aerobic biomineralization of a-hexachlorocyclohexane in a contaminated calcareous soil. Environ Sci Technol 24: 1349–1354
Rosenberg M, Rosenberg E (1981) Role of adherence in growth of Acinetobacter calcoaceticus RAG-1 on hexadecane. J Bacteriol 148: 51–57
Ryan JN, Elimelech M, Ard RA, Harvey RW, Johnson PR (1999) Bacteriophage PRD1 and silica colloid transport and recovery in iron oxide-coated sand aquifer. Environ Sci Technol 33: 63–73
Schmidt SK, Alexander M, Shuler ML (1985) Predicting threshold concentrations of organic substrates for bacterial growth. J Theor Biol 114: 1–8
Schwarzenbach RP, Gschwend PM, Imboden DM (1993) Environmental Organic Chemistry. John Wiley & Sons, Inc, New York, NY
Scow KM, Johnson CR (1997) Effect of sorption on biodegradation of soil pollutants. Adv Agron 58: 168–223
Stelmack PL, Gray MT, Pickard MA (1999) Bacterial adhesion to soil contaminants in the presence of surfactants. Appl Environ Microbiol 65: 163–168
Stucki G, Alexander M (1987) Role of dissolution and solubility in biodegradation of aromatic compounds. Appl Environ Microbiol 53: 292–297
Tang WC, White JC, Alexander M (1998) Utilization of sorbed compounds by microorganisms isolated for that purpose. Appl Microbiol Technol 49: 117–121
Tate III RL (1995) Soil Microbiology. John Wiley & Sons, Inc, New York, Chichester, Brisbane, Toronto, Singapore
Thomas JM, Alexander M (1987) Colonization and mineralization of palmitic acid by Pseudomonas pseudoflava. Microb Ecol 14: 75–80
Thomas JM, Yordy JR, Amador JA, Alexander M (1986) Rates of dissolution and biodegrada-tion of water-insoluble organic compounds. Appl Environ Microbiol 52: 290–296
Tiehm A (1994) Degradation of polycyclic aromatic hydrocarbons in the presence of synthetic surfactants. Appl Environ Microbiol 60: 258–263
Tiehm A, Fritzsche C (1995) Utilization of solubilized and crystalline mixtures of polycyclic aromatic hydrocarbons by a Mycobacterium sp. Appl Microbiol Biotechnol 42: 964–968
Tongpim S, Pickard MA (1996) Growth of Rhodococus S1 on anthracene. Can J Microbiol 42: 289–294
Tros ME, Schraa G, Zehnder AJB, Bosma TNP (1998) Anomalies in the transformation of 3chlorobenzoate in percolation columns with Pseudomonas sp. strain B13. Water Sci Tech 37: 89–96
Uden van N (1967) Transport-limited growth in the chemostat and its competitive inhibition; a theoretical treatment. Arch Microbiol 58: 145–154
Volkering F, Breure AM, Andel van JG (1993) Effect of micro-organisms on the bioavailability and biodegradation of crystalline naphthalene. Appl Microbiol Biotechnol 40: 535–540
Volkering F, Breure AM, Andel van JG, Rulkens WH (1995) Influence of nonionic surfactants on bioavailability and biodegradation of polycyclic aromatic hydrocarbons. Appl Environ Microbiol 61: 1699–1705
Volkering F, Breure AM, Rulkens WH (1998) Microbial aspects of surfactant use for biological soil remediation. Biodegradation 8: 401–417
Volkering F, Breure AM, Strekenburg A, Andel JGv (1992) Microbial degradation of polycyclic aromatic hydrocarbons: effect of substrate availability on bacterial growth kinetics. Appl Microbiol Biotechnol 36: 548–552
Weast RC (ed) (1984) Handbook of Chemistry and Physics, 64th edition. CRC Press, Inc, Boca Raton
Willumsen PA, Karlson U (1997) Screening of bacteria, isolated from PAH-contaminated soils, for production of biosurfactants and bioemulsifiers. Biodegradation 7: 415–423
Willumsen PA, Karlson U, Pritchard PH (1998) Response of fluoranthene-degrading bacteria to surfactants. Appl Microbiol Biotechnol 50: 475–483
Wodzinski RS, Bertolini D (1972) Physical state in which naphthalene and biphenyl are utilized by bacteria. Appl Microbiol 23: 1077–1081
Wodzinski RS, Coyle JE (1974) Physical state of phenanthrene for utilization by bacteria. Appl Microbiol 27: 1081–1084
Wodzinski RS, Johnson MJ (1968) Yields of bacterial cells from hydrocarbons. Appl Microbiol 16: 1886–1891
Zhang WX, Bouwer EJ, Ball WP (1998) Bioavailability of hydrophobic organic contaminants: effects and implications of sorption-related mass transfer on bioremediation. Ground Water Monit R 18: 126–138
Zhang Y, Maier WJ, Miller RM (1997) Effect of rhamnolipids on the dissolution, bioavailability, and biodegradation of phenanthrene. Environ Sci Technol 31: 2211–2217
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Wick, L.Y., Springael, D., Harms, H. (2001). Bacterial Strategies to Improve the Bioavailability of Hydrophobic Organic Pollutants. In: Stegmann, R., Brunner, G., Calmano, W., Matz, G. (eds) Treatment of Contaminated Soil. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04643-2_15
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