Abstract
Compartmentation of plant constituents is an important means of regulating plant metabolism (Dennis and Miernyk 1982). Perhaps the most significant breakthrough in the last 10 years in this field has been the use of protoplasts as a source for cell fractionation experiments and organelle isolations. Although protoplasts were first isolated using cell-wall-degrading enzymes in 1960 (Cocking 1960), it was not until the mid 1970’s that functional organelles were isolated from protoplasts (Galun 1981; Nishimura et al. 1976; Rathnam and Edwards 1976; Wagner and Siegelman 1975). Since that time, protoplasts have been used in hundreds of studies of plant metabolism.
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Thayer, S.S. (1985). Protoplasts — for Compartmentation Studies. In: Linskens, HF., Jackson, J.F. (eds) Cell Components. Modern Methods of Plant Analysis, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82587-3_3
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DOI: https://doi.org/10.1007/978-3-642-82587-3_3
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