Abstract
Direct somatic embryogenesis is the formation of somatic embryos or embryo-genic tissue directly from the explant without the formation of an intermediate callus phase (Raghavan 1986). In embryogenesis systems, this is almost always what happens (Merkle et al. 1990; Finer 1994). The production of a callus usually is undesirable if embryogenesis is the goal of the research. Two known exceptions to direct embryogenesis are carrot (see Chap. 7) and alfalfa, where a mass of cells that is relatively unorganized can form embryos after the proper manipulations. Carrot and alfalfa have become regular and useful systems for embryogenesis studies due to the relative ease of producing plants in vitro. Unfortunately, in most other plants somatic embryogenesis is more difficult to obtain, but good progress is being made (Attree and Fowke 1993).
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Armstrong CL, Green CE (1985) Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta 164: 207–214
Attree SM, Fowke LC (1993) Embryogeny of gymnosperms: advances in synthetic seed technology of conifers. Plant Cell Tissue Organ Cult 35: 1–35
Finer JJ (1988) Apical proliferation of embryogenic tissue of soybean [Glycine max (L.) Merrill]. Plant Cell Rep 7: 238–241
Finer JJ (1994) Plant regeneration via embryogenic suspension cultures. In: Dixon RA, Gonzales RA (eds) Plant cell culture: a practical approach. Oxford University Press, Oxford, pp 67–102
Lazzeri PA, Hildebrand DF, Collins GB (1985) A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant Mol Biol Rep 3: 160–167
Merkle SA, Parrott WA, Williams EG (1990) Applications of somatic embryogenesis and embryo cloning. In: Bhojwani SS (ed) Plant tissue culture: applications and limitations. Elsevier, Amsterdam, pp 67–102
Nehra NS, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M, Kartha KK (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J 5: 285–297
Raghavan V (1986) Embryogenesis in angiosperms: a developmental and experimental study. Cambridge University Press, New York
Vain P, Yean H, Flament P (1989) Enhancement of production and regeneration of embryogenic type II callus in Zea mays L. by AgNO3. Plant Cell Tissue Organ Cult 18: 143–151
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© 1995 Springer-Verlag Berlin Heidelberg
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Finer, J.J. (1995). Direct Somatic Embryogenesis. In: Gamborg, O.L., Phillips, G.C. (eds) Plant Cell, Tissue and Organ Culture. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79048-5_8
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DOI: https://doi.org/10.1007/978-3-642-79048-5_8
Publisher Name: Springer, Berlin, Heidelberg
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