Abstract
Among the DNA lesions induced by ionizing radiations, DNA double-strand breaks (DSBs) appear to be the most important, leading to chromosomal aberrations and cell death. We have presented biochemical and cytological evidence for this conclusion. These include the increase in the frequeny of chromosomal aberrations and DSBs in CHO cells X-irradiated and posttreated with Neurospora endonuclease, an enzyme which specifically cuts single-stranded DNA, thus generating DSBs (Natarajan and Obe 1978; Natarajan et al. 1980). In addition, restriction endonucleases, which induce only one type of lesion, namely DSBs, induce chromosomal aberrations in a pattern similar to that induced by ionizing radiations, i.e., chromosome type of aberrations in G1 and chromatid type of aberrations following G2 treatment (Natarajan and Obe 1984, Obe and Winkel 1985).
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© 1990 Springer-Verlag Berlin Heidelberg
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Natarajan, A.T., Vyas, R.C., Darroudi, F., Mullenders, L.H.F., Zdzienicka, M.Z. (1990). DNA Lesions, DNA Repair, and Chromosomal Aberrations. In: Obe, G., Natarajan, A.T. (eds) Chromosomal Aberrations. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75682-5_4
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DOI: https://doi.org/10.1007/978-3-642-75682-5_4
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