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Immunocytochemical Visualization of the Microtubular Cytoskeleton in Developing Kernels of Wheat (Triticum aestivum)

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Sexual Reproduction in Higher Plants

Abstract

Morphogenesis is based upon processes such as cell differentiation and tissue interaction. In a series of studies concerning the function of the microtubular (MT) cytoskeleton during plant cell development various functions of microtubules (MTs) were established in developing pollen (Van Lammeren et al. 1985), megaspores and embryo sacs of Gasteria (Willemse and Van Lammeren 1988), in developing somatic embryos of Daucus (Van Lammeren et al. 1987) and in developing endosperm of Zea (Van Lammeren & Kieft 1987). Because of the impermeability of cell membranes for antibodies, used for the immunological detection of MTs, tissues were first embedded, then sectioned and after the removal of the embedding medium processed for immuno labelling.

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References

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© 1988 Springer-Verlag Berlin Heidelberg

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van Lammeren, A.A.M. (1988). Immunocytochemical Visualization of the Microtubular Cytoskeleton in Developing Kernels of Wheat (Triticum aestivum). In: Cresti, M., Gori, P., Pacini, E. (eds) Sexual Reproduction in Higher Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73271-3_64

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  • DOI: https://doi.org/10.1007/978-3-642-73271-3_64

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73273-7

  • Online ISBN: 978-3-642-73271-3

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