Abstract
Immunoprecipitation (IP) and immunoblotting (Western blotting) are potential tools for addressing questions about the expression of a protein and its interactions with other proteins. Immunoprecipitation and immunoblotting are, as their names imply, techniques dependent on immunological agents — namely antibodies. These techniques take advantage of the high specificity and tight binding that antibodies provide to separate the desired proteins out from the background of all the other cellular proteins present. Immunoprecipitation has long been used as a tool for assessing a protein’s presence, while immunoblotting provides a more accurate means of quantitating the amount of the protein that is present. Immunoblotting also serves to establish or verify the identity of proteins isolated in immunoprecipitation. A vast number of other questions can also be addressed by these two related techniques.
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© 1996 Springer-Verlag Berlin Heidelberg
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Jenkins, C.W., Xiong, Y. (1996). Immunoprecipitation and Immunoblotting in Cell Cycle Studies. In: Pagano, M. (eds) Cell Cycle — Materials and Methods. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57783-3_22
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DOI: https://doi.org/10.1007/978-3-642-57783-3_22
Publisher Name: Springer, Berlin, Heidelberg
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