Abstract
The in vivo Matrigel angiogenesis assay, or Matrigel plug assay, was developed to examine neovascularization in rodents in response to specific angiogenic factors, independently of the presence of tumor cells (Kibbey et al. 1992, Passaniti et al. 1992, Grant et al. 1993, Capogrossi and Passaniti 1999). Since its inception, a variety of agents have been used to deliver an angiogenic stimulus, including recombinant cytokines, adenoviral vectors, low dose tumor cells, and even telomerase-transfected endothelial cells (Muhlhauser et al. 1995, Klement et al. 2000, Akhtar et al. 2001, Yang et al. 2001). The applications of the assay include (1) the study of the basic mechanisms underlying angiogenesis at the molecular or cellular level and (2) the in vivo evaluation of the activities of angiogenic and angiostatic compounds or cytokines (Capogrossi and Passaniti 1999).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Akhtar N, Carlson S, Pesarini A, Ambulos N, Passaniti A (2001) Extracellular matrix-derived angiogenic factor(s) inhibit endothelial cell proliferation, enhance differentiation, and stimulate angiogenesis in vivo. Endothelium 8:221–234
Capogrossi M, Passaniti A (1999) An in vivo angiogenesis assay to study positive and negative regulators of neovascularization; Chap. 30: in Methods in molecular medicine, vascular disease: molecular biology and gene therapy protocols, ed. by Baker AH, Humana Press Inc., Totowa, NJ. pp. 367–384
Grant DS, Kleinman HK, Goldberg ID, Bhargava MM, Nickoloff BJ, Kinsella JL, Polverini P, Rosen EM (1993) Scatter factor induces blood vessel formation in vivo. Proc Natl Acad Sci U S A 90:1937–1941
Kibbey MC, Grant DS, Kleinman HK (1992) Role of the SIKVAV site of laminin in promotion of angiogenesis and tumor growth: an in vivo Matrigel model. J Natl Cancer Inst 84:1633–1638
Klement G, Baruchel S, Rak J, Man S, Clark K, Hicklin DJ, Bohlen P, Kerbel RS (2000) Continuous low-dose therapy with vinblastine and VEGF receptor-2 antibody induces sustained tumor regression without overt toxicity. J Clin Invest 105:R15–R24
Muhlhauser J, Pili R, Merrill MJ, Maeda H, Passaniti A, Crystal RG, Capogrossi MC (1995) In vivo angiogenesis induced by recombinant adenovirus vectors coding either for secreted or non-secreted forms of acidic fibroblast growth factor. Hum Gene Ther 6:1457–1465
Passaniti A, Taylor RM, Pili R, Guo Y, Long PV, Haney JA, Pauly RR, Grant DS, Martin GR (1992) A simple, quantitative method for assessing angiogenesis and anti-angiogenic agents using reconstituted basement membrane, heparin, and FGF. Lab Invest 67:519–528
Pili R, Guo Y, Chang J, Nakanishi H, Martin GR, Passaniti A (1994) Altered angiogenesis underlying age-dependent changes in tumor growth. J Natl Cancer Inst 86:1303–1314
Vukanovic J, Passaniti A, Hirato T, Traysman RJ, Isaacs JT (1992) Anti-angiogenic effects of the Quinoline-3-carboxamide, linomide. Cancer Res 53:1833–1837
Yang J, Nagavarapu U, Relloma K, Sjaastad M, Moss WC, Passaniti A, Herron GS (2001) Telomerized human microvasculature is functional in vivo. Nat Biotech 19:219–224
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Passaniti, T., Vitolo, M.I. (2004). In Vivo Matrigel Angiogenesis Assays. In: Augustin, H.G. (eds) Methods in Endothelial Cell Biology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18725-4_20
Download citation
DOI: https://doi.org/10.1007/978-3-642-18725-4_20
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-21397-0
Online ISBN: 978-3-642-18725-4
eBook Packages: Springer Book Archive