Abstract
This chapter describes recent studies in which the quartz crystal microbalance (QCM) technology has been applied as a monitoring tool for animal cells in vitro. With shear wave resonators used as growth substrates it is possible to follow the de novo formation or the modulation of established cell-substrate contacts from readings of the resonance frequency with a time resolution in the order of seconds. From cell adhesion studies it became clear that different cell types induce an individual shift of the resonance frequency but it has been a matter of debate, which subcellular structures determine the individual impact of a given cell type on the QCM response. This question has been addressed by our group in recent years and a summary of our current understanding of this problem will be given here. Different approaches have been applied to challenge the cells in a well-defined way and to monitor the associated changes of the QCM readout. Taken together, these studies have led us to the following conclusions: (i) The cellular bodies primarily lead to an increased energy dissipation that does not correspond to a simple viscous behavior. (ii) The adhesive proteins underneath the cells provide a measurable contribution to the overall QCM response of adherent cells. (iii) The average distance between lower cell membrane and substrate surface does not have a significant impact on the acoustic load situation. (iv) The QCM is sensitive to cell stiffness and reports in a similar way on changes in cell stiffness, as accessible from scanning force microscopy measurements. (v) The cortical actin cytoskeleton is a dominant contributor to the cells’ acoustic response.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Keywords
References
Martin SJ, Granstaff VE, Frye GC (1991) Anal Chem 63:2272
Martin SJ, Frye GC, Ricco AJ (1993) Anal Chem 65:2910
Buttry D, Ward MD (1992) Chem Rev 92:1355
Bottom VE (1982) van Nostrand Reinhold Company, New York
Martin A, Hager HE (1989) J Appl Phys 65:2630
Sauerbrey G (1959) Z Phys 155:206
Janshoff A, Steinem C, Sieber M, el Baya A, Schmidt MA, Galla HJ (1997) Eur Biophys J 26:261
Janshoff A, Steinem C, Sieber M, Galla H-J (1996) Eur Biophys J 25:93
Gryte DM, Ward MD, Hu WS (1993) Biotechnol Prog 9:105
Redepenning J, Schlesinger TK, Mechalke EJ, Puleo DA, Bizios R (1993) Anal Chem 65:3378
Li J, Thielemann C, Reuning U, Johannsmann D (2005) Biosens Bioelectron 20:1333
Wegener J, Janshoff A, Galla HJ (1999) Eur Biophys J 28:26
Zhou T, Marx KA, Warren M, Schulze H, Braunhut SJ (2000) Biotechnol Prog 16:268
Marx KA, Zhou T, Montrone A, Schulze H, Braunhut SJ (2001) Biosens Bioelectron 16:773
Hug TS (2003) Assay Drug Dev Technol 1:479
Wegener J, Seebach J, Janshoff A, Galla HJ (2000) Biophys J 78:2821
Cans AS, Hook F, Shupliakov O, Ewing AG, Eriksson PS, Brodin L, Orwar O (2001) Anal Chem 73:5805
Bongrand P (1998) J Dispersion Sci Technol 19:963
Bell GI, Dembo M, Bongrand P (1984) Biophys J 45:1051
Pierres A, Benoliel AM, Bongrand P (2002) Eur Cells Mat 3:31
Vogler EA, Bussian (1987) J Biomed Mat Res 21:1197
Pierschbacher MD, Ruoslahti E (1987) J Biol Chem 262:17294
Pierschbacher MD, Ruoslahti E (1984) Nature 309:30
Reiss B, Janshoff A, Steinem C, Seebach J, Wegener J (2003) Langmuir 19:1816
Pignataro B, Steinem C, Galla HJ, Fuchs H, Janshoff A (2000) Biophys J 78:487
Luthgens E, Herrig A, Kastl K, Steinem C, Reiss B, Wegener J, Pignataro B, Janshoff A (2003) Meas Sci Technol 14:1865
Rodahl M, Höök F, Krozer A, Brzezinski P, Kasemo B (1995) Rev Sci Instrum 66:3924
Voinova MV, Jonson M, Kasemo B (2002) Biosens Bioelectron 17:835
Pignataro B, Steinem C, Galla HJ, Fuchs H, Janshoff A (2000) Biophys J 78:487
Rosenbaum JF (1988) Acoustic wave theory and devices. Artech House, Boston
Bandey HL, Martin SJ, Cernosek RW, Hillman AR (1999) Anal Chem 71:2205
Bandey HL, Hillman AR, Brown MJ, Martin SJ (1997) Faraday Discuss 107:105
Janshoff A, Wegener J, Sieber M, Galla HJ (1996) Eur Biophys J 25:93
Kanazawa KK, Gordon JG (1985) Anal Chem 57:1770
Lambacher A, Fromherz P (1996) Appl Phys A 63:207
Braun D, Fromherz P (1998) Phys Rev Lett 81:5241
Braun D, Fromherz P (1997) Appl Phys A 65:341
Marx KA, Zhou T, Montrone A, McIntosh D, Braunhut SJ (2005) Anal Biochem 343:23
Insall R, Machesky L (2001) Encyclopedia of life sciences. Wiley, Chichester
Brenner SL, Korn ED (1979) J Biol Chem 254:9982
Rotsch C, Braet F, Wisse E, Radmacher M (1997) Cell Biol Int 21:685
Hofmann UG, Rotsch C, Parak WJ, Radmacher M (1997) J Struct Biol 119:84
Bereiter-Hahn J, Karl I, Luers H, Voth M (1995) Biochem Cell Biol 73: 337
Shroff SG, Saner DR, Lal R (1995) Am J Physiol 269:C286
Hutter JL, Chen J, Wan WK, Uniyal S, Leabu M, Chan BM (2005) J Microsc 219:61
Rotsch C, Jacobson K, Radmacher M (1999) Proc Natl Acad Sci USA 96:921
Hoheisel D, Nitz T, Franke H, Wegener J, Hakvoort A, Tilling T, Galla HJ (1998) Biochem Biophys Res Commun 244:312
Schrot S, Weidenfeller C, Schaffer TE, Robenek H, Galla HJ (2005) Biophys J 89:3904
Castellino F, Heuser J, Marchetti S, Bruno B, Luini A (1992) Proc Natl Acad Sci USA 89:3775
Giaever I, Keese CR (1993) Nature 366:591
Giaever I, Keese CR (1991) Proc Natl Acad Sci USA 88:7896
Wegener J, Hakvoort A, Galla HJ (2000) Brain Res 853:115
Wegener J, Keese C, Giaever I (2002) Biotechniques 33:348
Wegener J (2003) Encyclopedia of life sciences. Wiley, Chichester
Pax M, Rieger J, Eibl RH, Thielemann C, Johannsmann D (2005) Analyst 130:1474
Sapper A, Wegener J, Janshoff A (2006) Anal Chem 78:5184
Dultsev FN, Ostanin VP, Klenerman D (2000) Langmuir 16:5036
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Heitmann, V., Reiß, B., Wegener, J. (2006). The Quartz Crystal Microbalance in Cell Biology: Basics and Applications. In: Janshoff, A., Steinem, C. (eds) Piezoelectric Sensors. Springer Series on Chemical Sensors and Biosensors, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-36568-6_9
Download citation
DOI: https://doi.org/10.1007/978-3-540-36568-6_9
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-36567-9
Online ISBN: 978-3-540-36568-6
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)