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Biolog Phenotype MicroArrays for Phenotypic Characterization of Microbial Cells

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Environmental Microbiology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1096))

Abstract

Biolog Phenotype MicroArrays for microorganisms provide a high-throughput method for the global analysis of microbial growth phenotypes. Using a colorimetric reaction that is indicative of respiration, these microplate assays measure the response of an individual strain or microbial community to a large and diverse range of nutrients and chemicals. Phenotype MicroArrays have been used to study gene function and to improve genome annotation in single microorganisms and for physiological profiling of bacterial communities. The microplate system can be used to obtain a comprehensive overview of metabolic capability, or it can be tailored, through the use of subsets of plates, to address specific research needs.

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Mackie, A.M., Hassan, K.A., Paulsen, I.T., Tetu, S.G. (2014). Biolog Phenotype MicroArrays for Phenotypic Characterization of Microbial Cells. In: Paulsen, I., Holmes, A. (eds) Environmental Microbiology. Methods in Molecular Biology, vol 1096. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-712-9_10

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  • DOI: https://doi.org/10.1007/978-1-62703-712-9_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-711-2

  • Online ISBN: 978-1-62703-712-9

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