Skip to main content
SpringerLink
Log in

Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord

  • Protocol
  • First Online:
Neural Progenitor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1059))

Abstract

Our group recently provided evidence for the presence of neural stem cells and/or progenitor cells in the adult human spinal cord. In this chapter, we review materials and methods to harvest high-quality samples of thoracolumbar, lumbar, and sacral adult human spinal cord from brain-dead patients who had agreed to donate their bodies to science for therapeutic and scientific advances. The methods to culture precursor cells from the adult human spinal cord are also described.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Dromard C et al (2008) Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro. J Neurosci Res 86:1916–1926

    Article  PubMed  CAS  Google Scholar 

  2. Mamaeva D et al. (2011) Isolation of mineralizing Nestin+ Nkx6.1+ vascular muscular cells from the adult human spinal cord. BMC Neurosci Oct 10;12:99

    Google Scholar 

  3. Perrin FE et al. (2011) Anatomical study of serotonergic innervation and 5-HT(1A) receptor in the human spinal cord. Cell Death Dis Oct 13;2:e218

    Google Scholar 

  4. Camby C (2008) The French Biomedicine Agency and medically assisted reproduction. Bull Acad Natl Med 192:17–21

    PubMed  Google Scholar 

  5. French Biomedicine Agency (2010) http://www.agence-biomedecine.fr/uploads/document/doc_agenceva.pdf

  6. Tixier D, Barrou B (2007) Recommandations techniques pour le prélèvement des organes et des tissus sur donneurs en état de mort encéphalique. http://www.edimark.fr/publications/articles/prelevement-des-organes-et-des-tissus-sur-donneurs-en-etat-de-mort-encephalique/13358

Download references

Author information

Authors and Affiliations

  1. Département de Neurochirurgie, Centre Hospitalier Universitaire, Hôpital Gui de Chauliac, Montpellier, France

    Luc Bauchet & Nicolas Lonjon

  2. Institut des Neurosciences de Montpellier, INSERM U1051, Université Montpellier 2, Hôpital St Eloi, Montpellier, France

    Luc Bauchet

  3. Centre Mutualiste Neurologique Propara, Montpellier, France

    Luc Bauchet

  4. Coordination Hospitalière de Prélèvement et Agence de Biomédecine, Hôpital Gui de Chauliac, Centre Hospitalier Universitaire, Montpellier, France

    Florence Vachiery-Lahaye

  5. Département d’Anesthésie Réanimation, DAR C, Centre Hospitalier Universitaire, Hôpital Gui de Chauliac, Montpellier, France

    Alain Boularan

  6. Institut des Neurosciences de Montpellier, INSERM U1051, Université Montpellier 2, Hôpital St Eloi, 74103 80, Montpellier, France

    Alain Privat

  7. Hopital Saint Eloi, INSERM U1051, Institute for Neuroscience of Montpellier (INM), University Montpellier 2, Montpellier, France

    Jean-Philippe Hugnot

Authors
  1. Luc Bauchet
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nicolas Lonjon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Florence Vachiery-Lahaye
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alain Boularan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Alain Privat
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jean-Philippe Hugnot
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Neurosurgery, University of Florida, Gainesville, Florida, USA

    Brent A. Reynolds

  2. Department of Neurosurgery, University of Florida, Gainesville, Florida, USA

    Loic P. Deleyrolle

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media New York

About this protocol

Cite this protocol

Bauchet, L., Lonjon, N., Vachiery-Lahaye, F., Boularan, A., Privat, A., Hugnot, JP. (2013). Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord. In: Reynolds, B., Deleyrolle, L. (eds) Neural Progenitor Cells. Methods in Molecular Biology, vol 1059. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-574-3_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-574-3_8

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-573-6

  • Online ISBN: 978-1-62703-574-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation