Abstract
Peptide nucleic acids (PNAs) are synthetic oligonucleotide analogues based on a pseudopeptide backbone that bind complementary DNA or RNA with high affinity and specificity. In this chapter, three PNA-based genotyping assays are described: PCR clamping, fluorescence-based recognition, and microarray platform. The first two methods are performed in solution, while the microarray method uses a solid surface.
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Sforza, S., Tedeschi, T., Bencivenni, M., Tonelli, A., Corradini, R., Marchelli, R. (2014). Use of Peptide Nucleic Acids (PNAs) for Genotyping by Solution and Surface Methods. In: Nielsen, P., Appella, D. (eds) Peptide Nucleic Acids. Methods in Molecular Biology, vol 1050. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-553-8_12
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DOI: https://doi.org/10.1007/978-1-62703-553-8_12
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