Abstract
Annexin A1 (Anxa1) is a highly specific surface marker of tumor vasculature. We used peptide-displaying phage technology to identify a carbohydrate ligand-mimicking 7-mer peptide, IFLLWQR (IF7), which can target Anxa1 in tumor vasculature. Here, we describe the binding activity of carbohydrate to Anxa1, Anxa1 to heparan sulfates, and the therapeutic potential of IF7 conjugated with anticancer drugs in tumor targeting.
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References
Hakomori S (2002) Glycosylation defining cancer malignancy: new wine in an old bottle. Proc Natl Acad Sci USA 99:10231–10233
Nakamori S, Kameyama M, Imaoka S, Furukawa H, Ishikawa O, Sasaki Y, Kabuto T, Iwanaga T, Matsushita Y, Irimura T (1993) Increased expression of sialyl Lewisx antigen correlates with poor survival in patients with colorectal carcinoma: clinicopathological and immunohistochemical study. Cancer Res 53:3632–3637
Fukuda MN, Ohyama C, Lowitz K, Matsuo O, Pasqualini R, Ruoslahti E, Fukuda M (2000) A peptide mimic of E-selectin ligand inhibits sialyl Lewis X-dependent lung colonization of tumor cells. Cancer Res 60:450–456
Fukuda MN (2006) Screening of peptide-displaying phage libraries to identify short peptides mimicking carbohydrates. Methods Enzymol 416:51–60
Hatakeyama S, Sugihara K, Nakayama J, Akama TO, Wong SM, Kawashima H, Zhang J, Smith DF, Ohyama C, Fukuda M, Fukuda MN (2009) Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Proc Natl Acad Sci USA 106:3095–3100
Oh P, Li Y, Yu J, Durr E, Krasinska KM, Carver LA, Testa JE, Schnitzer JE (2004) Subtractive proteomic mapping of the endothelial surface in lung and solid tumours for tissue-specific therapy. Nature 429:629–635
Sasisekharan R, Shriver Z, Venkataraman G, Narayanasami U (2002) Roles of heparan-sulphate glycosaminoglycans in cancer. Nat Rev Cancer 2:521–528
Ono K, Ishihara M, Ishikawa K, Ozeki Y, Deguchi H, Sato M, Hashimoto H, Saito Y, Yura H, Kurita A, Maehara T (2002) Periodate-treated, non-anticoagulant heparin-carrying polystyrene (NAC-HCPS) affects angiogenesis and inhibits subcutaneous induced tumour growth and metastasis to the lung. Br J Cancer 86:1803–1812
Horlacher T, Noti C, de Paz JL, Bindschadler P, Hecht ML, Smith DF, Fukuda MN, Seeberger PH (2011) Characterization of annexin A1 glycan binding reveals binding to highly sulfated glycans with preference for highly sulfated heparan sulfate and heparin. Biochemistry 50:2650–2659
Lehr HA, Leunig M, Menger MD, Nolte D, Messmer K (1993) Dorsal skinfold chamber technique for intravital microscopy in nude mice. Am J Pathol 143:1055–1062
Meyer-Losic F, Nicolazzi C, Quinonero J, Ribes F, Michel M, Dubois V, de Coupade C, Boukaissi M, Chene AS, Tranchant I, Arranz V, Zoubaa I, Fruchart JS, Ravel D, Kearsey J (2008) DTS-108, a novel peptidic prodrug of SN38: in vivo efficacy and toxicokinetic studies. Clin Cancer Res 14:2145–2153
Sckell A, Leunig M (2001) Dorsal skinfold chamber preparation in mice: studying angiogenesis by intravital microscopy. Methods Mol Med 46:95–105
Sckell A, Leunig M (2009) The dorsal skinfold chamber: studying angiogenesis by intravital microscopy. Methods Mol Biol 467:305–317
Gori JL, Podetz-Pedersen K, Swanson D, Karlen AD, Gunther R, Somia NV, McIvor RS (2007) Protection of mice from methotrexate toxicity by ex vivo transduction using lentivirus vectors expressing drug-resistant dihydrofolate reductase. J Pharmacol Exp Ther 322:989–997
Hatakeyama S, Yamamoto H, Ohyama C (2010) Tumor formation assays. Methods Enzymol 479:397–411
Acknowledgments
This study was supported by a grant-in-aid for Scientific Research 23791737 from the Japan Society for the Promotion of Science (SH) and NIH grant P01CA071932 (MNF), DoD Breast Cancer Research IDEA grant DAMD17-02-1-0311 (MNF), and a Susan Komen Breast Cancer Research grant BCTR0504175 (MNF).
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Hatakeyama, S., Shibata, T.K., Tobisawa, Y., Ohyama, C., Sugihara, K., Fukuda, M.N. (2013). Tumor Targeting by a Carbohydrate Ligand-Mimicking Peptide. In: Brockhausen, I. (eds) Glycosyltransferases. Methods in Molecular Biology, vol 1022. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-465-4_28
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DOI: https://doi.org/10.1007/978-1-62703-465-4_28
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Publisher Name: Humana Press, Totowa, NJ
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