Abstract
Fluorescence In Situ Hybridization (FISH) is a powerful and robust technique allowing the visualization of target sequences like genes in interphase nuclei. It is widely used in routine diagnostics to identify cancer specific aberrations including lymphoma associated translocations or gene copy number changes in single tumor cells. By combining FISH with immunophenotyping—a technique called Fluorescence Immunophenotyping and Interphase Cytogenetic as a Tool for Investigation Of Neoplasia (FICTION)—it is moreover possible to identify a cell population of interest. Here we describe standard protocols for FISH and FICTION as used in our laboratory in diagnosis and research.
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Acknowledgments
The work of the authors on FISH/FICTION has been supported by the Deutsche Krebshilfe, the Wilhelm Sander Stiftung, the Kinderkrebsinitiative Buchholz/Holm-Seppensen, the Jose Carreras foundation, the BMBF, and the Polish Ministry of Science and Higher Education (grant N301 238736). MG was supported by the FEBS long-term fellowship and the Support for International Mobility of Scientists fellowships of the Polish Ministry of Science and Higher Education. We thank Reina Zühlke-Jenisch and Ursula Schnaidt for excellent technical assistance.
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Giefing, M., Siebert, R. (2013). FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas. In: Küppers, R. (eds) Lymphoma. Methods in Molecular Biology, vol 971. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-269-8_13
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DOI: https://doi.org/10.1007/978-1-62703-269-8_13
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Publisher Name: Humana Press, Totowa, NJ
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