Abstract
Chemoresistance and increased growth rate are two gain-of-function functions that mutant p53 is thought to possess. Here, we describe two methods for measuring the sensitiveness of cells to chemotherapeutic drugs and the rate of cell growth. Both of which can be used with a wide range of cell types. The clonogenic assay can be used with many different chemotoxic drugs and the growth assay described here presents an alternative to the MTT assay and allows for a long-term measurement of cell growth. These protocols are both easy, flexible, require relatively little effort, and are inexpensive to carry out.
These authors contributed equally to this work.
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Acknowledgments
This work was supported by grants from NIH to Sumitra Deb (CA70712 and CA121144) and Swati Palit Deb (CA74172), and Pilot Project Awards from Massey Cancer to Sumitra Deb and Swati Palit Deb. We thank Shilpa Singh for help in editing this chapter.
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Ramamoorthy, M., Vaughan, C., Deb, S., Deb, S.P. (2013). Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells. In: Deb, S., Deb, S. (eds) p53 Protocols. Methods in Molecular Biology, vol 962. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-236-0_10
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DOI: https://doi.org/10.1007/978-1-62703-236-0_10
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