Abstract
Sheath blight disease of rice caused by the soilborne fungal pathogen Rhizoctonia solani has been a major disease of rice with a serious threat to stable rice production worldwide. Although various cultural practices have been used to manage the disease, it is advantageous and important to screen rice germplasm and identify resistant rice cultivars for more effective disease control. Recent advances in methods for the fungal inoculation and disease evaluation have enabled a better measurement of host resistance by minimizing confounding factors from plant architectures and environmental conditions. This chapter introduces five such methods: (1) detached leaf method; (2) micro-chamber method; (3) mist-chamber method; (4) parafilm sachet method; and (5) aluminum foil method. These methods are useful for screening and evaluating disease reactions of rice germplasm and facilitating the genetic mapping of disease resistance genes.
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References
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Acknowledgements
The authors thank Ellen McWhirter for proofreading, Anna McClung of USDA-ARS Dale Bumpers National Rice Research Center, and Fernando Correa-Victoria of RiceTec for technical assistance. This work was supported in part by the USDA Cooperative State Research, Education and Extension Service—National Research Initiative—Applied Plant Genomics Program (USDA/CSREES grant 2004-35317-14867) and by USDA-ARS National Program NP301 project “Response of Diverse Rice Germplasm to Biotic and Abiotic Stresses.”
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Jia, Y., Liu, G., Park, DS., Yang, Y. (2013). Inoculation and Scoring Methods for Rice Sheath Blight Disease. In: Yang, Y. (eds) Rice Protocols. Methods in Molecular Biology, vol 956. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-194-3_19
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DOI: https://doi.org/10.1007/978-1-62703-194-3_19
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-193-6
Online ISBN: 978-1-62703-194-3
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