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Nested PCR and RFLP Analysis Based on the 16S rRNA Gene

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Phytoplasma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 938))

Abstract

Current phytoplasma detection and identification methods are primarily based on nested polymerase chain reaction followed by restriction fragment length polymorphism analysis and gel electrophoresis. These methods can potentially detect and differentiate all phytoplasmas including those previously not described. The present protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels.

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Author information

Authors and Affiliations

  1. Laboratory of Applied Phytopathology, Institute of Pesticides and Environmental Protection, Belgrade, Serbia

    Bojan Duduk

  2. DiSTA, Plant Pathology, Alma Mater Studiorum—University of Bologna, Bologna, Italy

    Samanta Paltrinieri & Assunta Bertaccini

  3. Molecular Plant Pathology Laboratory, USDA, ARS, Beltsville, MD, USA

    Ing-Ming Lee

Authors
  1. Bojan Duduk
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  2. Samanta Paltrinieri
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  3. Ing-Ming Lee
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  4. Assunta Bertaccini
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Corresponding author

Correspondence to Bojan Duduk .

Editor information

Editors and Affiliations

  1. School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Nottingham, LE12 5RD, United Kingdom

    Matt Dickinson

  2. The Food and Environment Research Agency, Sand Hutton, YO41 1LZ, United Kingdom

    Jennifer Hodgetts

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Duduk, B., Paltrinieri, S., Lee, IM., Bertaccini, A. (2013). Nested PCR and RFLP Analysis Based on the 16S rRNA Gene. In: Dickinson, M., Hodgetts, J. (eds) Phytoplasma. Methods in Molecular Biology, vol 938. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-089-2_14

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  • DOI: https://doi.org/10.1007/978-1-62703-089-2_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-088-5

  • Online ISBN: 978-1-62703-089-2

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