Abstract
Xenotransplantation is one alternative to transplantation of human organs which has been investigated. It is generally accepted that the pig represents the most logical choice of animals to serve as organ donors for xenotransplantation. Moreover, the implementation of cloning by somatic cell nuclear transfer (SCNT) and transgenic techniques have resulted in the production of numerous transgenic pigs than can be used for xenotransplantation purposes as well as models for human diseases.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Lunney JK (2007) Advances in swine biomedical model genomics. Int J Biol Sci 3:179–184
Te Riele H, Maandag ER, Berns A (1992) Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc Natl Acad Sci USA 89:5128–5132
Ramsoondar JJ, Machaty Z, Costa C, Williams BL, Fodor WL, Bondioli KR (2003) Production of α1,3-Galactosyltransferase-Knockout cloned pigs expressing human α1,2-Fucosylosyltransferase. Biol Reprod 69:437–445
Van den Hoff MJB, Moorman AFM, Lamers WH (1992) Electroporation in ‘intracellular’ buffer increases cell survival. Nucleic Acids Res 20:2902–2905
Takeda T, Hasler JF (1986) Effect of plastic disposable syringes on development of mouse embryos in culture. Theriogenology 25:205
Bondioli KR, Hill KG (1986) The effect of exposing media to syringes on the viability of bovine embryos. Theriogenology 25:142
Zheng YS, Sirard MA (1992) The effect of sera, bovine serum albumin and follicular cells on in vitro maturation and fertilization of porcine oocytes. Theriogenology 37:779–790
Yoshida M, Ishizaki Y, Kawagishi H, Bamba K, Kojima Y (1992) Effects of pig follicular fluid on maturation of pig oocytes in vitro and on their subsequent fertilizing and developmental capacity in vitro. J Reprod Fertil 95:481–488
Wang W, Niwa K (1995) Synergetic effects of epidermal growth factor and gonadotropins on the cytoplasmic maturation of pig oocytes in a serum-free medium. Zygote 3:345–350
Abeydeera LR, Wang WH, Prather RS, Day BN (1998) Maturation in vitro of pig oocytes in protein-free culture media: fertilization and subsequent embryo development in vitro. Biol Reprod 58:1316–1320
Petters RM, Wells KD (1993) Culture of pig embryos. J Reprod Fertil 48:61–73
Dobrinsky JR, Johnson LA, Rath D (1996) Development of a culture medium (BECM-3) for porcine embryos: effects of bovine serum albumin and fetal bovine serum on embryo development. Biol Reprod 55:1069–1074
Yoshioka K, Suzuki C, Tanaka A, Anas IM, Iwamura S (2002) Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol Reprod 66:112–119
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Giraldo, A.M., Ball, S., Bondioli, K.R. (2012). Production of Transgenic and Knockout Pigs by Somatic Cell Nuclear Transfer. In: Costa, C., Máñez, R. (eds) Xenotransplantation. Methods in Molecular Biology, vol 885. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-845-0_8
Download citation
DOI: https://doi.org/10.1007/978-1-61779-845-0_8
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-844-3
Online ISBN: 978-1-61779-845-0
eBook Packages: Springer Protocols