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Collection, Processing, and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine

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Somatic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 879))

Abstract

Collection and banking of umbilical cord blood can provide a virtually unlimited source of ethnically diverse stem cell donors. It can be used in place of bone marrow or peripheral blood stem cells for hematologic transplants as well as in a variety of regenerative medicine applications. In this study, we review the latest developments in cord blood banking. We have banked over 300,000 collections at our facility, which were processed by either Ficoll or AXP methodologies. An average 95–99% processing efficiency was obtained. Processed samples can be frozen in either cryovials or bags and banked in the vapor phase of a liquid nitrogen dewar for prolonged periods of time. In conclusion, it is possible to simply and reproducibly harvest, process, and bank cord blood samples using currently available technology.

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Abbreviations

AXP:

AutoXpress™ Platform

BMT:

Bone marrow transplantation

CB:

Cord blood

CBR:

Cord Blood Registry

DMSO:

Dimethylsulfoxide

GVHD:

Graft-versus-host disease

HLA:

Human leukocyte antigen

IDM:

Infectious disease marker

MNC:

Mononuclear cells

TNC:

Total nucleated cells

References

  1. Hows JM (1987) Histocompatible unrelated donors for bone marrow transplantation. Bone Marrow Transplant 1:259–263

    PubMed  CAS  Google Scholar 

  2. Bradley BA, Gilks WR, Gore SM, Klouda PT (1987) How many HLA typed volunteer donors for bone marrow transplantation (BMT) are needed to provide an effective service? Bone Marrow Transplant 2(suppl 1):79

    Google Scholar 

  3. Sullivan KM, Weiden PL, Storb R et al (1989) Influence of acute and chronic graft-versus-host disease on relapse and survival after bone marrow transplantation from HLA-identical siblings as treatment of acute and chronic leukemia. Blood 73:1720–1728

    PubMed  CAS  Google Scholar 

  4. Broxmeyer HE, Gluckman E, Auerbach A et al (1990) Human umbilical cord blood: a clinically useful source of transplantable hematopoietic stem/progenitor cells. Intl J Cell Cloning 8(suppl 1):76

    Article  Google Scholar 

  5. Gluckman E, Broxmeyer HE, Auerbach A et al (1989) Hematopoietic reconstitution in a patient with Fanconi’s anemia by means of umbilical cord blood from an HLA-identical sibling. N Engl J Med 321:1174–1178

    Article  PubMed  CAS  Google Scholar 

  6. Gluckman E (1990) Stem cell harvesting from cord blood: a new perspective. In: Henon PR, Wunder EW (eds) Peripheral blood stem cell autografts. Springer, Berlin

    Google Scholar 

  7. Broxmeyer HE, Kurtzburg J, Gluckman E et al (1991) Umbilical cord blood hematopoietic stem and repopulating cells in human clinical transplantation: an expanded role for cord blood transplantation. Blood Cells 17: 330–337

    Google Scholar 

  8. Broxmeyer HE, Kurtzburg J, Gluckman E et al (1991) Umbilical cord blood hematopoietic stem and repopulating cells in human clinical transplantation. Blood Cells 17:313–330

    PubMed  CAS  Google Scholar 

  9. Broxmeyer HE, Douglas GW, Hangoc G et al (1989) Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells. Proc Natl Acad Sci U S A 86:3828–3832

    Article  PubMed  CAS  Google Scholar 

  10. Vilmer E, Sterkers G, Rahimy C et al (1992) HLA-mismatched cord blood transplantation in a patient with advanced leukemia. Transplantation 53:1155–1157

    Article  PubMed  CAS  Google Scholar 

  11. Wagner JE, Kernan NA, Steinbuch M et al (1995) Allogeneic sibling umbilical cord blood transplantation in children with malignant and nonmalignant disease. Lancet 346:214–219

    Article  PubMed  CAS  Google Scholar 

  12. Rubinstein P, Rosenfield RE, Adamson JW, Stevens CE (1993) Stored placental blood for unrelated bone marrow reconstitution. Blood 81:1679–1690

    PubMed  CAS  Google Scholar 

  13. Gluckman E, Rocha V, Boyer-Chammard A (1997) Outcome of cord-blood transplantation from related and unrelated donors. N Engl J Med 337(6):373–381

    Article  PubMed  CAS  Google Scholar 

  14. Rubinstein P (2006) Why cord blood? Hum Immunol 67(6):398–404

    Article  PubMed  CAS  Google Scholar 

  15. McGuckin C, Forraz N, Baradez MO et al (2005) Production of stem cells with embryonic characteristics from human umbilical cord blood. Cell Prolif 38:245–255

    Article  PubMed  CAS  Google Scholar 

  16. McGuckin CP, Forraz N, Allouard Q, Pettengell R (2004) Umbilical cord blood stem cells can expand hematopoietic and neuroglial progenitors in vitro. Exp Cell Res 295:350–359

    Article  PubMed  CAS  Google Scholar 

  17. Rogers I, Yamanaka N, Bielecki R, Wong CJ, Chua S, Yuen S, Casper RF (2007) Identification and analysis of in vitro cultured CD45-positive cells capable of multi-lineage differentiation. Exp Cell Res 313:1839–1852

    Article  PubMed  CAS  Google Scholar 

  18. Kucia M, Halasa M, Wysoczynski M et al (2007) Morphological and molecular characterization of novel population of CXCR4+ SSEA-4+ Oct-4+ very small embryonic-like cells purified from human umbilical cord blood-preliminary report. Leukemia 21:297–303

    Article  PubMed  CAS  Google Scholar 

  19. Harris DT, He X, Badowski M, Nichols JC (2008) Regenerative medicine of the eye: a short review. In: Levicar N, Habib NA, Dimarakis I, Gordon MY (eds) Stem cell repair and regeneration, vol 3. Imperial College Press, London, pp 211–225

    Chapter  Google Scholar 

  20. Sunkomat JNE, Goldman S, Harris DT (2007) Cord blood-derived MNCs delivered intracoronary contribute differently to vascularization compared to CD34+ cells in the rat model of acute ischemia. J Mol Cell Cardiol 42(6 suppl 1):S97

    Article  Google Scholar 

  21. Harris DT, Badowski M, Ahmad N, Gaballa M (2007) The potential of cord blood stem cells for use in regenerative medicine. Expert Opin Biol Ther 7(9):1311–1322

    Article  PubMed  CAS  Google Scholar 

  22. Harris DT, Rogers I (2007) Umbilical cord blood: a unique source of pluripotent stem cells for regenerative medicine. Curr Stem Cell Res Ther 2:301–309

    Article  PubMed  CAS  Google Scholar 

  23. Harris DT, Schumacher MJ, LoCascio J et al (1992) Phenotypic and functional immaturity of human umbilical cord blood T lymphocytes. Proc Natl Acad Sci U S A 89:10006–10010

    Article  PubMed  CAS  Google Scholar 

  24. Harris DT, Schumacher MJ, LoCascio J et al (1994) Immunoreactivity of umbilical cord blood and post-partum maternal peripheral blood with regard to HLA-haploidentical transplantation. Bone Marrow Transplant 14:63–68

    PubMed  CAS  Google Scholar 

  25. Harris DT, LoCascio J, Besencon FJ (1994) Analysis of the alloreactive capacity of human umbilical cord blood: implications for graft-versus-host disease. Bone Marrow Transplant 14:545–553

    Article  PubMed  CAS  Google Scholar 

  26. Harris DT (1995) In vitro and in vivo assessment of the graft-versus-leukemia activity of cord blood. Bone Marrow Transplant 15:17–23

    PubMed  CAS  Google Scholar 

  27. Harris DT (1994) GVL and GVHD implications of cord blood. Proceedings of the international conference/workshop on cord blood transplantation and biology/immunology. Blood Cells 20:560–565

    PubMed  CAS  Google Scholar 

  28. Harris DT, Schumacher MJ, Rychlik S et al (1994) Collection, separation and cryopreservation of umbilical cord blood for use in transplantation. Bone Marrow Transplant 13:135–143

    PubMed  CAS  Google Scholar 

  29. Harris DT (1994) What every physician needs to know about cord blood banking. Round-Up (Maricopa County Medical Society News), Dec 1994

    Google Scholar 

  30. Harris DT (1996) Experience in autologous and allogeneic cord blood banking. J Hematother 5(2):123–128

    Article  PubMed  CAS  Google Scholar 

  31. Harris DT (1997) Cord blood banking for transplantation. Can J Clin Med 4:1–8

    Google Scholar 

  32. Harris DT (1998) Cord blood banking. The University of Arizona experience: successes, problems and cautions. Cancer Res Ther Control 7:63–67

    Google Scholar 

  33. Kielpinski G, Prinzi S, Duguid J, du Moulin G (2005) Roadmap to approval: use of an automated sterility test method as a lot release test for Carticel, autologous cultured chondrocytes. Cytotherapy 7(6):531–541

    Article  PubMed  CAS  Google Scholar 

  34. Papassavas AC, Goika V, Chatzistamatiou T et al (2008) A strategy of splitting individual high volume cord blood units into two half subunits prior to processing increases the recovery of cells and facilitates ex vivo expansion of the infused hematopoietic progenitor cells in adults. Int J Lab Hematol 30(2):124–132

    Article  PubMed  CAS  Google Scholar 

  35. Harris DT, McGaffey AP, Schwarz RH et al (2007) Comparing the mononuclear cell (MNC) recovery of AXP and Hespan. Obstet Gynecol 109(4):93S

    Google Scholar 

  36. AABB (2007) Standards for cellular therapy product services, 2nd edn. AABB Press, Bethesda

    Google Scholar 

  37. Lane TA, Plunkett M, Buenviaje J, Law P et al (2002) Recovery of leukocytes in cord blood units after cryopreservation by controlled rate freeze in DMSO and storage in vapor phase liquid nitrogen. In: Poster, ISCT conference

    Google Scholar 

  38. Harris DT, Mapother M, Goodman C (2000) Prevention of cross-sample and infectious contamination during cord blood banking by use of cryovials for storage in liquid nitrogen. Transfusion 40(10S):111S

    Google Scholar 

  39. 2020: A new vision—a future for regenerative medicine. http://www.dhhs.gov/reference/newfuture.shtml. Retrieved on 12 Apr 2007

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Acknowledgments

I would like to acknowledge the invaluable technical assistance all of the Cord Blood Bank personnel that have made this study possible. I would also like to acknowledge the numerous physicians, midwifes, and nurses that have participated in the collection of the cord blood samples.

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Authors and Affiliations

  1. Department of Immunobiology, University of Arizona, Tucson, AZ, USA

    Michael S. Badowski & David T. Harris

Authors
  1. Michael S. Badowski
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  2. David T. Harris
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Corresponding author

Correspondence to David T. Harris .

Editor information

Editors and Affiliations

  1. Mouse Cancer Genetics Program, National Cancer Institute, Center Drive 31, Frederick, 21702, Maryland, USA

    Shree Ram Singh

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Badowski, M.S., Harris, D.T. (2012). Collection, Processing, and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_16

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  • DOI: https://doi.org/10.1007/978-1-61779-815-3_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-814-6

  • Online ISBN: 978-1-61779-815-3

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