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Engineering the Caenorhabditis elegans Genome by Mos1-Induced Transgene-Instructed Gene Conversion

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Mobile Genetic Elements

Part of the book series: Methods in Molecular Biology ((MIMB,volume 859))

Abstract

Mos1-induced transgene-instructed gene conversion (MosTIC) is a technique of choice to engineer the genome of the nematode Caenorhabditis elegans. MosTIC is initiated by the excision of Mos1, a DNA transposon of the Tc1/Mariner super family that can be mobilized in the germ line of C. elegans. Mos1 excision creates a DNA double-strand break that is repaired by several cellular mechanisms, including transgene-instructed gene conversion. For MosTIC, the transgenic repair template used by the gene conversion machinery is made of sequences that share DNA homologies with the genomic region to engineer and carries the modifications to be introduced in the genome. In this chapter, we present two MosTIC protocols routinely used.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular and Cellular Biology, Ecole Normale Supérieure de Lyon, Lyon, France

    Valérie J. P. Robert

Authors
  1. Valérie J. P. Robert
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Correspondence to Valérie J. P. Robert .

Editor information

Editors and Affiliations

  1. , Physiologie de la Reproduction, UMR INRA-CNRS 6175, n/a, Nouzilly Cedex, 37380, France

    Yves Bigot

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Robert, V.J.P. (2012). Engineering the Caenorhabditis elegans Genome by Mos1-Induced Transgene-Instructed Gene Conversion. In: Bigot, Y. (eds) Mobile Genetic Elements. Methods in Molecular Biology, vol 859. Humana Press. https://doi.org/10.1007/978-1-61779-603-6_11

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  • DOI: https://doi.org/10.1007/978-1-61779-603-6_11

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-602-9

  • Online ISBN: 978-1-61779-603-6

  • eBook Packages: Springer Protocols

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