Abstract
Here, we present methods for extracting DNA and RNA from samples of whole bone tissue and culture bone cells and describe methods quantitative and qualitative measurement of the extracted nucleic acids. These protocols described provide high-quality nucleic acids suitable for downstream applications such as quantitative PCR and microarrays.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Chomczynski, P., and Sacchi, N. (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.
Blin, N., and Stafford, D. W. (1976) A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acid Res. 3, 2303–2308.
Kupiec, J. J., Giron, M. L., Vilette, D., Jeltsch, J. M., and Emanoil-Ravier, R. (1987) Isolation of high molecular weight DNA from eukaryotic cells by formamide treatment and dialysis. Anal. Biochem. 164, 53–59.
Bowtell, D. D. L. (1987) Rapid isolation of eukaryotic DNA. Anal. Biochem. 162, 463–465.
Vogelstein, B., and Gillespie, D. (1979) Preparative and analytical purification of DNA from agarose. Proc. Natl. Acad. Sci. USA. 76, 615–619.
Sambrook, J., Fritsch, E.F., and Maniatis, T. (2000) Molecular Cloning. A laboratory Manual. 3rd Edition, Cold Spring Harbour Laboratory Press.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Hughes, A., Stewart, T.L., Mann, V. (2012). Extraction of Nucleic Acids from Bone. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_17
Download citation
DOI: https://doi.org/10.1007/978-1-61779-415-5_17
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-414-8
Online ISBN: 978-1-61779-415-5
eBook Packages: Springer Protocols