Abstract
Microtubules, polymers of the heterodimeric protein αβ-tubulin, give shape to cells and are the tracks for vesicle transport and chromosome segregation. In vitro assays to study microtubule functions and their regulation by microtubule-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.
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Acknowledgements
The authors acknowledge the input of many people in the development of these protocols. Protocols published on the Web, especially by the Mitchison lab, have proved invaluable in the development of this protocol. In more recent years, many members, past and present, of the Howard, Diez, and Hyman labs at the MPI-CBG have been involved in performing this purification. In particular, we thank Regine Hartmann, Corina Braeuer, Marija Zanic, Gero Fink, Tony Ashford, and Andy Hunter. Finally, C.G. and C.T.F. thank Gary Brouhard, both for sharing the misery of getting this protocol up and running (again) and for promising (after “the incident”) never to run another PC column.
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Gell, C., Friel, C.T., Borgonovo, B., Drechsel, D.N., Hyman, A.A., Howard, J. (2011). Purification of Tubulin from Porcine Brain. In: Straube, A. (eds) Microtubule Dynamics. Methods in Molecular Biology, vol 777. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-252-6_2
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DOI: https://doi.org/10.1007/978-1-61779-252-6_2
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