Abstract
The number of techniques to identify, quantify and characterise cell death is rapidly increasing as more is known about the complex mechanisms underlying this process. However, most of these techniques are invasive and require preparation steps such as cell fixation, staining or protein extractions. Non-invasive analysis of living cells represents a key point in cell biology, e.g. in toxicology studies or in tissue engineering. In this chapter, we report the usefulness of Raman spectroscopy as a non-invasive method to distinguish cells at different stages of cell cycle and living cells from dead cells. Throughout two examples, we show the performance and the use of Raman spectroscopy as a new non-invasive method to assess cell viability.
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References
Taylor, R.C., Cullen, S.P., Martin, S.J. (2008) Apoptosis, controlled demolition at the -cellular level. Nat. Rev. Mol. Cell Biol. 9, 231–241.
Heeres, J.T., Hergenrother, P.J. (2007) Poly(ADP-ribose) makes a date with death. Curr Opin Chem Biol. 11, 644–653.
Tait, J. (2008) Imaging of apoptosis. J. Nucl. Med. 49, 1573–1576
Balasubramanian, K., Schroit, A.J. (2003) Aminophospholipid asymmetry: a matter of life and death. Annu. Rev. Physiol. 65, 701–734
Laserna, J. (2005) An introduction to Raman Spectroscopy: Introduction and basic principles. spectroscopyNOW.com
Notingher, I., Hench, L.L. (2006) Raman microspectroscopy: a non-invasive toll for studies of individual living cells in vitro. Exp. Rev. Med. Dev., 3, 215–234
Notingher, I., Verrier, S., Haque, S., Polak, J.M.P., Hench, L.L. (2003) Spectroscopic study of human lung epithelial cells (A549) in culture: living cells versus dead cells. Biopolymers, 72, 230–240.
Notingher, I., Selvakumaran, J., Hench L.L. (2004) New Detection System for Toxic Agents Based on Continuous Spectroscopic Monitoring of Living Cells. Biosensors and Bioelectronics 20, 780–789
Uzunbajakava, N., Lenferink, A., Kraan, Y., Volokhina, E., Vrensen, G., Greve, J., and Otto, C. (2003) Nonresonant confocal raman imaging of DNA and protein distribution in apoptotic cells, Biophysical Journal 84, 3968–3981.
Verrier, S., Notingher, I., Polak, J.M., and Hench, L.L. (2004) In situ monitoring of cell death using raman microspectroscopy, Biopolymers, 74, 157–162.
Zoladek, A., Pascut, F.C., Patel, P., and Notingher, I., (2010 submitted) Non-invasive time-course imaging of apoptotic cells by Confocal Raman micro-spectroscopy, J Raman Spectrosc, DOI 10.1002/jrs.2707 (in press).
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Verrier, S., Zoladek, A., Notingher, I. (2011). Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test. In: Stoddart, M. (eds) Mammalian Cell Viability. Methods in Molecular Biology, vol 740. Humana Press. https://doi.org/10.1007/978-1-61779-108-6_19
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DOI: https://doi.org/10.1007/978-1-61779-108-6_19
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Online ISBN: 978-1-61779-108-6
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