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Liquid Hot Water Pretreatment of Cellulosic Biomass

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Biofuels

Part of the book series: Methods in Molecular Biology ((MIMB,volume 581))

Summary

Lignocellulosic biomass is an abundant and renewable resource for fuel ethanol production. However, the lignocellulose is recalcitrant to enzymatic hydrolysis because of its structural complexity. Controlled-pH liquid hot water (LHW) pretreatment of cellulosic feedstock improves its enzymatic digestibility by removing hemicellulose and making the cellulose more accessible to cellulase enzymes. The removed hemicellulose is solubilized in the liquid phase of the pretreated feedstock as oligosaccharides. Formation of monomeric sugars during the LHW pretreatment is minimal. The LHW pretreatment is carried out by cooking the feedstock in process water at temperatures between 160 and 190°C and at a pH of 4–7. No additional chemicals are needed. This chapter presents the detailed procedure of the LHW pretreatment of lignocellulosic biomass.

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Acknowledgment

This chapter was internally reviewed by Stefan Schwietzke and John Schumm. The material in this work was supported by DOE Grants #DE-FG36–04G01417, #G012O26–174 and USDA IFAFS contract # 00–52104–9663. Michael Ladisch is Chief Technology Officer at Mascoma Corporation, a cellulose ­biofuels company.

Author information

Authors and Affiliations

  1. Department of Agricultural and Biological Engineering, and Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN, USA

    Youngmi Kim, Nathan S. Mosier & Michael R. Ladisch

  2. Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN, USA

    Rick Hendrickson

Authors
  1. Youngmi Kim
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  2. Rick Hendrickson
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  3. Nathan S. Mosier
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  4. Michael R. Ladisch
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Editor information

Editors and Affiliations

  1. Bioconversion Science & Technology, Oak Ridge National Laboratory, Bethel Valley Road 1, Oak Ridge, 37831-6341, U.S.A.

    Jonathan R. Mielenz

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Kim, Y., Hendrickson, R., Mosier, N.S., Ladisch, M.R. (2009). Liquid Hot Water Pretreatment of Cellulosic Biomass. In: Mielenz, J. (eds) Biofuels. Methods in Molecular Biology, vol 581. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-214-8_7

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  • DOI: https://doi.org/10.1007/978-1-60761-214-8_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-213-1

  • Online ISBN: 978-1-60761-214-8

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