Abstract
Microscopy studies of oxidative stress have often consisted of only immunocytochemical localization or only cytochemical localization studies. However, correlative studies on the same sections provide more useful data in interpreting oxidative and nitrosative stress. Cellular sites of superoxide and hydrogen peroxide production can be identified using cerium-based cytochemical localization of NADH oxidase enzymatic activity, while areas of nitrosative stress can be identified by immunocytochemical localization of nitrotyrosine in the same sections of tissue.
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Williamson, J. R., Chang, K., Frangos, M., Hasan, K. S., Ido, I., Kawamura, T., Nyengaard, J. R., Van den Enden, M., Kilo, C., and Tilton, R. G. (1993) Hyperglycemic pseudohypoxia and diabetic complications. Diabetes 42, 801–813.
Beckman, J. S., Ye, Y. Z., Anderson, P., Chen, J., Accavetti, M. A., Tarpey, M. M., and White, C. R. (1994) Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Biol. Chem. Hoppe-Seyler 375, 81–88.
Ellis, E. A., Sengupta, N., Caballero, S., Guthrie, S. M., Mames, R. N., and Grant, M. B. (2005) Nitric oxide synthases modulate progenitor and resident endothelial cell behavior in galactosemia. Antioxid. Redox Signal. 7, 1413–1422.
Ellis, E. A., Guberski, D. L., Hutson, B., and Grant, M. B. (2002) Time course of NADH oxidase, inducible nitric oxide synthase and peroxynitrite in diabetic retinopathy in the BBZ/Wor rat. Nitric Oxide 6, 295–304.
Cave, A. C., Brewer, A. C., Narayanapanicker, A., Rasy, R., Grieve, D. J., Walker, S., and Shah, A. M. (2006) NADPH oxidases in cardiovascular health and disease. Antioxid. Redox Signal. 8, 691–728.
Briggs, R. T., Karnovsky, M. L., and Karnovsky, M. J. (1975) Localization of NADH oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method. J. Cell Biol. 67, 566–586.
Ellis, E. A., and Grant, M. B. (2002) Cytochemical localization of H2O2 in biological tissues, in Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols (Armstrong, D., ed.) Humana Press, Totowa, New Jersey, pp. 3–12.
Robinson, J. M. (1985) Improved localization of intracellular sites of phosphatases using cerium and cell permeabilization. J. Histochem. Cytochem. 33, 749–754.
Ellis, E. A. (2006) Solutions to the problem of substitution of ERL 4221 for vinyl cyclohexene dioxide in Spurr low viscosity embedding formulations. Microscopy Today 14, 32–33.
Briggs, R. T., Karnovsky, M. L., and Karnovsky, M. J. (1977) Hydrogen peroxide in chronic granulomatous disease: a cytochemical study of reduced pyridine nucleotide oxidases. J. Clin. Invest. 59, 1088–1098.
Ellis, E. A., Grant, M. B., Murray, F. T., Wachowski, M. B., Guberski, D. L., Kubalis, P. S., and Lutty, G. A. (1998) Increased NADH oxidase activity in the retina of the BBZ/Wor diabetic rat. Free Rad. Biol. Med. 24, 111–120.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Ellis, E.A. (2008). Correlative Transmission Microscopy: Cytochemical Localization and Immunocytochemical Localization in Studies of Oxidative and Nitrosative Stress. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress I. Methods In Molecular Biology, vol 477. Humana Press. https://doi.org/10.1007/978-1-60327-517-0_4
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DOI: https://doi.org/10.1007/978-1-60327-517-0_4
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