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Adeno-associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism

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Lipoproteins and Cardiovascular Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1027))

Abstract

Adeno-associated viral vectors have proven to be excellent gene delivery vehicles for somatic overexpression. These viral vectors can efficiently and selectively target the liver, which plays a central role in lipoprotein metabolism. Both liver-expressed as well as non-hepatic secreted proteins can be easily examined in different mouse models using this approach. The dosability of adeno-associated viral (AAV) vectors, as well as their potential for long-term expression, makes them an excellent choice for assessing gene function in vivo. This section will cover the use of AAV to study lipoprotein metabolism—including vector design, virus production and purification, and viral delivery, as well as monitoring of transgene expression and resulting phenotypic changes. Practical information is provided to assist the investigator in designing, interpreting, and troubleshooting experiments.

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Acknowledgments

The authors would like to thank Anne Douar at Genethon for her contributions to the genome titer assay method. We would also like to acknowledge Peter Bell who kindly provided the electron micrograph image of AAV shown in Fig. 1.

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Authors and Affiliations

  1. Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

    William R. Lagor & Daniel J. Rader

  2. Cardiovascular Institute, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

    William R. Lagor & Daniel J. Rader

  3. Penn Vector Core, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

    Julie C. Johnston, Martin Lock & Luk H. Vandenberghe

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  1. William R. Lagor
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  2. Julie C. Johnston
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  3. Martin Lock
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  4. Luk H. Vandenberghe
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Editors and Affiliations

  1. Pulmonary & Vascular Medicine Branch, National Institutes of Health (NIH) National Heart, Lung & Blood Institute, Bethesda, Maryland, USA

    Lita A. Freeman

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Lagor, W.R., Johnston, J.C., Lock, M., Vandenberghe, L.H., Rader, D.J. (2013). Adeno-associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism. In: Freeman, L. (eds) Lipoproteins and Cardiovascular Disease. Methods in Molecular Biology, vol 1027. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-369-5_13

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  • DOI: https://doi.org/10.1007/978-1-60327-369-5_13

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