Abstract
Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) is one of the most powerful tools for the separation of complex protein mixtures. This method separates individual proteins and polypeptide chains according to their isoelectric point and molecular weight. This chapter describes the protocol for 2D PAGE using carrier ampholyte pH gradient gel for the isoelectric focusing (IEF) separation (pH gradient 3.5–10.0) and a polyacrylamide gradient gel for the second dimension (1,2).
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References
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© 1996 Humana Press Inc., Totowa, NJ
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Gravel, P., Golaz, O. (1996). Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_20
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DOI: https://doi.org/10.1007/978-1-60327-259-9_20
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
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