Abstract
During the past two decades fluorescent in-situ hybridization (FISH) has become a standard technique to directly localize, orient, and order genes in the genomes of a wide range of species. Despite the availability of a variety of probes, probe labeling and signal-detection systems, and advanced image analysis software, the core procedures used to carry out FISH remain the same. A detailed overview of these procedures, including target preparation (metaphase/interphase chromosomes and DNA fibers), probe labeling, in-situ hybridization, signal detection, and imaging, is here provided in a stepwise manner.
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© 2008 Humana Press Inc., Totowa, NJ
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Raudsepp, T., Chowdhary, B.P. (2008). FISH for Mapping Single Copy Genes. In: Murphy, W.J. (eds) Phylogenomics. Methods in Molecular Biology™, vol 422. Humana Press. https://doi.org/10.1007/978-1-59745-581-7_3
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DOI: https://doi.org/10.1007/978-1-59745-581-7_3
Publisher Name: Humana Press
Print ISBN: 978-1-58829-764-8
Online ISBN: 978-1-59745-581-7
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