Summary
The reversible phosphorylation of serine, threonine, and tyrosine residues is one of the most important intracellular post-translational modifications regulating enzymatic activities and protein/protein interaction in eukaryotic cells. Tools for determining phosphorylation status of proteins and peptides play a prominent role in signal transduction research and proteomics. Pan-specific antibodies claimed to recognize modified amino acid residues independent on the nature of surrounding residues in peptides and proteins are widely used. We used high-content phosphopeptide microarrays and microarrays displaying acetyllysine-containing peptides for comprehensive characterization of commercially available generic anti-phosphopeptide and anti-acetyllysine antibodies. We were able to demonstrate distinct subsite specificity and cross-reactivity for such antibodies.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Zerweck, J., Masch, A., Schutkowski, M. (2009). Peptide Microarrays for Profiling of Modification State-Specific Antibodies. In: Schutkowski, M., Reineke, U. (eds) Epitope Mapping Protocols. Methods in Molecular Biology™, vol 524. Humana Press. https://doi.org/10.1007/978-1-59745-450-6_12
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DOI: https://doi.org/10.1007/978-1-59745-450-6_12
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