Abstract
Rodent arthritis models are tools to study joint destruction. This chapter describes methods to prepare and analyze histological sections from inflamed paws. Protocols to create decalcified paraffin-embedded as well as undecalcified plastic-embedded sections are provided. Strengths and limitations of these two histological techniques are described. Major staining techniques on hard tissue sections focusing on the evaluation of joint destruction are indicated. Finally, evaluation of arthritic paw sections by histomorphometry, a semiautomated computerized method to measure areas of interest, is described.
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References
Recker, R. (ed.) (1983) Bone histomorphometry: Techniques and Interpretation. CRC, Boca Raton, FL.
Vedi, S. and Compston, J. (2003) Bone Histomorphometry, in Bone Research Protocols, Ralston, SH, Helfrich, MH, eds., Humana Press, Totowa, NJ, pp. 283–298.
Aaron, J. E. and Shore., P. A. (2003) Bone histomorphometry: Concepts and common techniques, in Handbook for Histology Methods for Bone and Cartilage, An, Y. H., Martin, K. L., eds., Humana Press, Totowa, NJ, pp. 331–352.
Redlich, K., Gortz, B., Hayer, S., et al. (2004) Repair of local bone erosions and reversal of systemic bone loss upon therapy with anti-tumor necrosis factor in combination with osteoprotegerin or parathyroid hormone in tumor necrosis factor-mediated arthritis. Am. J. Pathol. 164, 543–555.
Scarano, A., Moreira, P. L., Kang, Q. K., and Gruber, H. E. (2003) Common fixatives in hard tissue technology, in Handbook for Histology Methods for Bone and Cartilage, An, Y. H., Martin, K. L., eds., Humana Press, Totowa, NJ, pp. 159–166.
Skinner, R. A. (2003) Decalcification of bone tissue, in Handbook for Histology Methods for Bone and Cartilage, An, Y. H., Martin, K. L., eds., Humana Press, Totowa, NJ, pp. 167–184.
An, Y. H., Moreira, P. L., Kang, Q. K., and Gruber, H. E. (2003) Principles of embedding and common protocols, in Handbook for Histology Methods for Bone and Cartilage, An, Y. H., Martin, K. L., eds., Humana Press, Totowa, NJ, pp. 185–198.
Gruber, H. E. and Ingram, J. A. (2003) Basic staining and histochemical techniques and immunohistochemical localization using bone sections, in Handbook for Histology Methods for Bone and Cartilage, An, Y. H., Martin, K. L., eds., Humana Press, Totowa, NJ, pp. 281–286.
Ries, W. L. (2003) Techniques for Sectioning undecalcified bone tissue using microtomes, in Handbook for Histology Methods for Bone and Cartilage, An, Y. H., Martin, K. L., eds., Humana Press, Totowa, NJ, pp. 221–232.
Scarano, A., Petrone, G., and Piattelli, A. (2003) Staining techniques of plastic embedded tissue specimen, in Handbook for Histology Methods for Bone and Cartilage, An, Y. H., Martin, K. L., eds., Humana Press, Totowa, NJ, pp. 315–320.
Parfitt, A. M., Drezner, M. K., Glorieux, F. H., et al. (1987) Bone Histomorphometry: Standardization of Nomenclature, Symbols and Units. J. Bone Miner. Res. 6, 595–610.
Zwerina, J., Hayer, S., Tohidast-Akrad, M., et al. (2004) Single and combined inhibition of tumor necrosis factor, interleukin-1, and RANKL pathways in tumor necrosis factor-induced arthritis: Effects on synovial inflammation, bone erosion, and cartilage destruction. Arthritis Rheum. 50, 277–290.
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© 2007 Humana Press Inc., Totowa, NJ
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Schett, G., Tuerk, B. (2007). Bone Histomorphometry in Arthritis Models. In: Cope, A.P. (eds) Arthritis Research. Methods in Molecular Medicine, vol 135. Humana Press. https://doi.org/10.1007/978-1-59745-401-8_17
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DOI: https://doi.org/10.1007/978-1-59745-401-8_17
Publisher Name: Humana Press
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