Summary
Microarray technology enables the fast and parallel analysis of a multitude of biologically relevant parameters. Not only nucleic acid-based tests, but also peptide, antigen, and antibody assays using different formats of microarrays evolved within the last decade. They offer the possibility to measure interactions in a miniaturised, economic, automated, and qualitative or quantitative way providing insights into the cellular machinery of diverse organisms. Examples of applications in research and diagnostics are, e.g., O-typing of pathogenic Escherichia coli, detection of bacterial toxins and other biological warfare agents (BW agents) from a variety of different samples, screening of complex antibody libraries, and epitope mapping. Conventional O- and H-serotyping methods can now be substituted by procedures applying DNA oligonucleotide and antibody-based microarrays. For simultaneous and sensitive detection of BW agents microarray-based tests are available, which include not only relevant viruses and bacteria, but also toxins. This application is not only restricted to the security and military sector but it can also be used in the fields of medical diagnostics or public health to detect, e.g., staphylococcal enterotoxins in food or clinical samples. Furthermore, the same technology could be used to detect antibodies against enterotoxins in human sera using a competitive assay. Protein and peptide microarrays can also be used for characterisation of antibodies. On one hand, peptide microarrays allow detailed epitope mapping. On the other hand, a set of different antibodies recognising the same antigen can be spotted as a microarray and labelled as detection antibodies. This approach makes it possible to test every combination, allowing to find the optimal pair of detection/capture antibody.
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References
1 . Graves PR , Haystead TA . (2002) Molecular biologist's guide to proteomics . Microbiol Mol Biol Rev 66 (1) , 39–63 .
2 . Xu Q , Lam KS . (2003) Protein and chemical microarrays–powerful tools for proteomics . J Biomed Biotechnol 2003 (5) , 257–66 .
3 . Monecke S , Slickers P , Hotzel H , et al. (2006) Microarray-based characterisation of a Panton-Valentine leukocidin-positive community-acquired strain of methicillin-resistant Staphylococcus aureus. Clin Micro-biol Infect 12 (8) , 718–28 .
4 . Sydor JR , Nock S . (2003) Protein expression profiling arrays: tools for the multiplexed high-throughput analysis of proteins . Proteome Sci 1 (1) , 3.
5 . Perlee L , Christiansen J , Dondero R , et al . (2004) Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics . Proteome Sci 2 (1) , 9.
6 . Woelfl S , Dummer A , Pusch L , Pfalz M , Wang L , Clement JH , Leube I , Ehricht R . (2004) Analyzing proteins and protein modifications with ArrayTube antibody microarrays . In: Schena M , ed. Protein Microarrays. Boston: Jones and Bartlett , 159–68.
7 . Ballmer K , Korczak BM , Kuhnert P , Slickers P , Ehricht R , Hachler H . (2007) Fast DNA-serotyping of Escherichia coli by oli-gonucleotide microarray . J Clin Microbiol 45 , 370–79
8 . Anjum MF , Tucker JD , Sprigings KA , Woodward MJ , Ehricht R . (2006) Use of miniaturized protein arrays for Escherichia coli O serotyping . Clin Vaccine Immunol 13 (5) , 561–7.
9 . Huelseweh B , Ehricht R , Marschall HJ. (2006) A simple and rapid protein array based method for the simultaneous detection of biowarfare agents . Proteomics 6 (10) , 2972–81 .
10 . Hülseweh B , Marschall HJ , Ehricht R. (2006) B-Kampfstoffe — Parallele und schnelle Analytik durch Antikörper-Arrays. Laborwelt 3 , 6–10 .
11 . Taitt CR , Anderson GP , Lingerfelt BM, Feldstein MJ , Ligler FS . (2002) Nine-ana-lyte detection using an array-based biosensor . Anal Chem 74 (23) , 6114–20.
12 . Rowe-Taitt CA , Golden JP , Feldstein MJ, Cras JJ , Hoffman KE , Ligler FS . (2000) Array biosensor for detection of biohazards . Biosens Bioelectron 14 (10–11) , 785–94.
13 . Ligler FS , Taitt CR , Shriver-Lake LC , Saps-ford KE , Shubin Y , Golden JP . (2003) Array biosensor for detection of toxins . Anal Bio-anal Chem 377 (3) , 469–77 .
14 . Andresen H , Zarse K , Grotzinger C , et al. (2006) Development of peptide microarrays for epitope mapping of antibodies against the human TSH receptor . J Immunol Methods 315 (1–2) , 11–18.
15 . Di Padova FE , Brade H , Barclay GR , et al. (1993) A broadly cross-protective monoclonal antibody binding to Escherichia coli and Salmonella lipopolysaccharides . Infect Immun 61 (9) , 3863–72 .
16 . Muller-Loennies S , Brade L , MacKenzie CR , Di Padova FE , Brade H . (2003) Identification of a cross-reactive epitope widely present in lipopolysaccharide from entero-bacteria and recognized by the cross-protective monoclonal antibody WN1 222-5 . J Biol Chem 278 (28) , 25618–27.
17 . Rusnak JM , Kortepeter M , Ulrich R , Poli M, Boudreau E . (2004) Laboratory exposures to staphylococcal enterotoxin B. Emerg Infect Dis 10 (9) , 1544–9 .
18 . Madsen JM . (2001) Toxins as weapons of mass destruction. A comparison and contrast with biological-warfare and chemical-warfare agents . Clin Lab Med 21 (3), 593–605 .
19 . Noda M , Hirayama T , Kato I , Matsuda F. (1980) Crystallization and properties of staphylococcal leukocidin . Biochim Biophys Acta 633 (1) , 33–44 .
20 . Gill SR , Fouts DE , Archer GL , et al. (2005) Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain. J Bacteriol 187 (7) , 2426–38 .
21 . Greiser-Wilke IM , Soine C , Moennig V. (1989) Monoclonal anibodies reacting specifically with Francisella sp . Zentralbl Veterinaermed B 36 , 593–600.
22 . Greiser-Wilke IM , Moennig V , Kaaden OR , Shope RE . (1991) Detection of alphaviruses in a genus-specific antigen capture enzyme immunoassay using monoclonal antibodies. J Clin Microbiol 29 (1) , 131–7.
23 . Greiser-Wilke IM , Moennig V . (1987) Monoclonal antibodies and characterization of epitopes of smooth Brucella lipopolysaccharides . Ann Inst Pasteur Microbiol 138 , 549–60.
24 . Johann S , Czerny CP . (1993) A rapid antigen capture ELISA for the detection of orthopox viruses . Zentralbl Veterinaermed B 40 , 569–81.
25 . Meyer H , Osterrieder N , Czerny CP . (1994) Identification of binding sites for neutralizing monoclonal antibodies on the 14-kDa fusion protein of orthopox viruses. Virology 200 (2) , 778–83.
26 . Harlow ELD . (1988) . Antibodies: A Laboratory Manual . New York : Cold Spring Harbor Laboratory Press.
27 . Kaerber G . (1931) Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche . Arch Exp Pathol Pharmakol 162, 480–3.
28 . Spearman C . (1908) The method of right and wrong cases (constant stimuli) without Gauss's formula . Brit J Psychol 2 , 227–42.
29 . Nicolson GL , Blaustein J . (1972) The interaction of Ricinus communis agglutinin with normal and tumor cell surfaces . Biochim Bio-phys Acta 266 (2) , 543–7.
Acknowledgments
We acknowledge helpful discussions by Anke Woestemeyer, Thomas Ellinger, and Marc Avondet. We are indebted to Elke Müller, Jana Sachtschal, Ines Engelmann, Antje Ruppelt, Heidi Kolata, Claudia Woidke, and Luzie Voß for technical assistance.
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Ehricht, R., Adelhelm, K., Monecke, S., Huelseweh, B. (2009). Application of Protein ArrayTubes to Bacteria, Toxin, and Biological Warfare Agent Detection. In: Bilitewski, U. (eds) Microchip Methods in Diagnostics. Methods in Molecular Biology™, vol 509. Humana Press. https://doi.org/10.1007/978-1-59745-372-1_6
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DOI: https://doi.org/10.1007/978-1-59745-372-1_6
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