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Measurement of 8-Iso-Prostaglandin F2α in Biological Fluids as a Measure of Lipid Peroxidation

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Cyclooxygenases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 644))

Abstract

Several lines of evidence suggest that reactive oxygen species are implicated in human disease, including atherosclerosis, hypertension, and restenosis after angioplasty. The measurement of F2-isoprostanes (F2-iPs), formed nonenzymatically through free radical catalyzed attack on esterified arachidonate, provides a reliable tool for identifying populations with enhanced rates of lipid peroxidation. Among F2-isoPs, 8-iso-PGF2α (also referred to IPF2α-III) and IPF2α-VI are the most frequently measured in biological fluids. A variety of methods have been proposed to measure F2-isoprostanes in urine and plasma. Mass spectrometry has been developed for the measurement of both F2-isoprostanes but its use is limited as it is time-consuming and highly expensive. We have developed validated enzyme immunoassay (EIA) and radioimmunoassay (RIA) techniques using highly specific antisera for the measurement of 8-iso-PGF2α. In contrast, the commercially available immunoassay kits are limited for their poor specificity. The measurement of specific isoprostanes, such as 8-iso-PGF2α, in urine is a reliable, noninvasive index of lipid peroxidation that is of valuable help in dose-finding studies of natural and synthetic antioxidant agents.

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Author information

Authors and Affiliations

  1. Department of Medicine and Center of Excellence on Aging, G. d’Annunzio University School of Medicine, and G. d’Annunzio University Foundation, Chieti, Italy

    Stefania Tacconelli & Marta L. Capone

  2. Sezione di Farmacologia, Dipartimento di Medicina e Scienze dell’Invecchiamento, Università di Chieti “G. d’Annunzio”, Chieti, Italy

    Paola Patrignani

Authors
  1. Stefania Tacconelli
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  2. Marta L. Capone
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  3. Paola Patrignani
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Editor information

Editors and Affiliations

  1. Queen Mary's School of Med. & Dentistry, William Harvey Research Inst., Barts & The London, Charterhouse Square, London, EC1M 6BQ, United Kingdom

    Samir S. Ayoub

  2. Queen Mary's School of Med. & Dentistry, William Harvey Research Inst., Barts & The London, Charterhouse Square, London, EC1M 6BQ, United Kingdom

    Roderick J. Flower

  3. Queen Mary's School of Med. & Dentistry, William Harvey Research Inst., Barts & The London, Charterhouse Square, London, EC1M 6BQ, United Kingdom

    Michael P. Seed

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Tacconelli, S., Capone, M.L., Patrignani, P. (2010). Measurement of 8-Iso-Prostaglandin F2α in Biological Fluids as a Measure of Lipid Peroxidation. In: Ayoub, S., Flower, R., Seed, M. (eds) Cyclooxygenases. Methods in Molecular Biology, vol 644. Humana Press. https://doi.org/10.1007/978-1-59745-364-6_14

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  • DOI: https://doi.org/10.1007/978-1-59745-364-6_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-953-6

  • Online ISBN: 978-1-59745-364-6

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