Summary
This chapter describes reliable flow cytometric methods for assessment of two important physiologic characteristics of bacteria, membrane potential and membrane permeability, which can provide indications of the effects of antimicrobial agents on microorganisms.
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References
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Acknowledgments
The author thanks Dave Novo, Nancy Perlmutter, and Jared Silverman, who have played vital roles in the development and application of the methods described here.
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Shapiro, H.M. (2008). Flow Cytometry of Bacterial Membrane Potential and Permeability. In: Champney, W.S. (eds) New Antibiotic Targets. Methods In Molecular Medicine™, vol 142. Humana Press. https://doi.org/10.1007/978-1-59745-246-5_14
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DOI: https://doi.org/10.1007/978-1-59745-246-5_14
Publisher Name: Humana Press
Print ISBN: 978-1-58829-915-4
Online ISBN: 978-1-59745-246-5
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