Summary
Chronic consumption of ethanol induces hepatic steatosis and inflammation, which can eventually lead to more severe liver injury, characterized by fibrosis and cirrhosis. Recruitment of neutrophils to the liver, as well as activation of Kupffer cells, mediates the inflammatory responses observed after chronic ethanol exposure. Kupffer cells, the resident macrophages of the liver, are critical to the onset of ethanol-induced liver injury. Activation of Kupffer cells leads to an increased production of proinflammatory cytokines, such as tumor necrosis factor-α and also reactive oxygen species, a process mediated in part by changes in lipopolysaccharide-induced TLR4-dependent signal transduction. The isolation and culture of Kupffer cells is an important technique with which one can elucidate the mechanisms that contribute to alcoholic liver injury.
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Acknowledgements
The work in this chapter was supported by National Institute on Alcohol Abuse and Alcoholism Grant no. AA-013868 and AA-011975 (to L.E.N.) and AA-015833 (to M.T.P.).
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McMullen, M.R., Pritchard, M.T., Nagy, L.E. (2008). Isolation of Kupffer Cells from Rats Fed Chronic Ethanol. In: Nagy, L.E. (eds) Alcohol. Methods in Molecular Biology™, vol 447. Humana Press. https://doi.org/10.1007/978-1-59745-242-7_15
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DOI: https://doi.org/10.1007/978-1-59745-242-7_15
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