Abstract
Several studies of DNA vaccination against HER2/neu showed the effectiveness of immunization protocols in models of transplantable or spontaneous tumors. The DNA delivery system plays a crucial role in the success of DNA vaccination. In particular, our studies of DNA vaccination against HER2/neu tumor antigen showed that intramuscular injection of the vaccine followed by electroporation elicits an optimal protection against the development of spontaneous HER2/neu- tumors occurring in transgenic mice.
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References
1. Tang, D., De Vit, M., and Johnston, S.A. (1992) Genetic immunization is a simple method for eliciting an immune response. Nature. 356,152–154.
2. Hynes, N.E. and Stern, D.F. (1994) The biology of erbB2/neu/HER2 and its role in cancer, BBA. 1198, 165–184.
3. Amici, A., Smorlesi, G., Noce, G., et al. (2000) DNA vaccination with full-length or truncated Neu induced protective immunity against the development of spontaneous mammary tumors in HER-2/neu transgenic mice. Gene Ther. 7, 703–706.
4. Quaglino, E., Rolla, S., Iezzi, M., et al. (2004) Concordant morphologic and gene expression data show that a vaccine halts HER2/neu preneoplastic lesions. J. Clin. Invest. 113, 709–717.
5. Conry, R.M., Curiel, D.T., Strong, T.V., et al. (2002) Safety and immunogenicity of DNA vaccine encoding carcinoembryonic antigen and hepatitis B surface antigen in colorectal carcinoma patients. Clin. Cancer Res. 8, 2782–2787.
6. Cappello, P., Triebel, F., Iezzi, M., et al. (2003) LAG-3 enables DNA vaccination to persistently prevent mammary carcinogenesis in HER-2/neu transgenic BALB/c mice. Cancer Res. 63, 2518–2525.
7. Disis, M.L., Scholler, N., Dahlin, A., et al. (2003) Plasmid-based vaccines encoding rat neu and immune stimulatory molecules can elicit rat neu-specific immunity. Mol. Cancer Ther. 2, 995–1002.
8. Renard, V., Sonderbye, L., Ebbehoj, K., et al. (2003) HER-2 DNA and protein vaccines containing potent Th cell epitopes induce distinct protective and therapeutic antitumor responses in HER-2 transgenic mice. J. Immunol. 171,1588–1595.
9. Rovero, S., Boggio, K., Carlo, E.D., et al. (2001) Insertion of the DNA for the 163–171 peptide of IL1beta enables a DNA vaccine encoding p185(neu) to inhibit mammary carcinogenesis in Her-2/neu transgenic BALB/c mice. Gene Ther. 8, 447–452.
10. Kirman, J.R. and Seder, R.A. (2003) DNA vaccination: the answer to stable, protective T-cell memory? Curr. Opin. Immunol. 15, 471–476.
11. Neumann, E., Schaefer-Ridder, M., Wang, Y., and Hofshneider, P.H. (1982) Gene transfer into mouse lymphoma cells by electroporation in high electric fields. EMBO J. 1, 841–845.
12. Smorlesi, A., Papalini, F., Amici, A., et al. (2006) Evaluation of different plasmid DNA delivery systems for immunization against HER2/neu in a transgenic murine model of mammary carcinoma Vaccine. 24, 1766–1775.
13. Quaglino, E., Iezzi, M., Mastini, C., et al. (2004) Electroporated DNA vaccine clears away multifocal mammary carcinomas in her-2/neu transgenic mice. Cancer Res. 64, 2858–2864.
14. Guy, C.T., Cardiff, R.D., and Muller, W.J. (1996) Activated neu induces rapid tumour progression. J. Biol. Chem. 271, 7673–7678.
15. Lollini, P.L., Nicoletti, G., Landuzzi, L., et al. (1998) Down regulation of major histocompatibility complex class I expression in mammary carcinoma of HER2/neu transgenic mice. Int. J. Cancer. 77, 937–941.
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© 2008 Humana Press
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Smorlesi, A., Papalini, F., Pierpaoli, S., Provinciali, M. (2008). HER2/neu DNA Vaccination for Breast Tumors. In: Li, S. (eds) Electroporation Protocols. Methods in Molecular Biology™, vol 423. Humana Press. https://doi.org/10.1007/978-1-59745-194-9_37
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DOI: https://doi.org/10.1007/978-1-59745-194-9_37
Publisher Name: Humana Press
Print ISBN: 978-1-58829-877-5
Online ISBN: 978-1-59745-194-9
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